Difference between revisions of "Team:TU Darmstadt/Demonstrate"

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<h3>Chitin Synthase NodC</h3>
 
<h3>Chitin Synthase NodC</h3>
 
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The functionality of the NodC enzyme was verified by performing the <i>UDP-Glo<sup>TM</sup> Glycosyltransferase</i> Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.  
 
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Revision as of 21:00, 29 October 2017

MainPage

Proof of Concept

Here we want to give you a short tour about what we achieved in the project. We show our results and give a view on the resulting product, a enzyme sensing hydrogel.

Chitin Synthase NodC

The functionality of the NodC enzyme was verified by performing the UDP-GloTM Glycosyltransferase Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.