Difference between revisions of "Team:ITB Indonesia/Model"

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<p>Here ODEs that we used :</p>
 
<p>Here ODEs that we used :</p>
  
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[[File:File.png|200px|thumb|left|alt text]]
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<p>Growth curve</p>
  
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<p>[[File:CodeCogsEqn.gif|thumb|center|Growth Formula]]</p>
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<img src="https://static.igem.org/mediawiki/2017/8/8b/CodeCogsEqn.gif" style="width: auto; height: auto; center"/>
  
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<p>[[File:CodeCogsEqn(1).gif|thumb|center|AI-2 Signaling]]</p>
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<p>AI-2 Production</p>
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<img src="https://static.igem.org/mediawiki/2017/d/de/CodeCogsEqn_%281%29.gif" style="width: auto; height: auto; center"/>
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<p>Biofilm Formation</p>
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<img src="https://static.igem.org/mediawiki/2017/f/f2/CodeCogsEqn_%282%29.gif" style="width: auto; height: auto; center"/>
  
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<p>[[File:CodeCogsEqn(2).gif|thumb|center|Biofilm Formation]]</p>
 
  
 
<h1 class="ITB_h1">Quorum Sensing</h1>
 
<h1 class="ITB_h1">Quorum Sensing</h1>

Revision as of 07:31, 30 October 2017

Team Attributions
Collaborations Overview Design Notebook Contribution Results Improve
Basic Parts Composite Part Part Collection
Silver HP Integrated and Gold Public Engagement


Modelling

Quorum Sensing

Quorum sensing mechanism was used to form biofilm of E.coli strain Top10, BL21 and DH5α that used in labwork. We have modeled growth curve of E.coli to determine when we must move E.coli colony from inoculum flask to reaction flask that contains PET. Besides growth curve, we have modeled some coupled ODEs to model growth curve, AI-2 production that affects signaling, and biofilm formation. AI-2 production in E.coli was used as colony signal of quorum sensing until it reaches specific points and finally form biofilm that affected by its quorum sensing by AI-2 signaling. We use Hill kinetics function as our approach to model AI-2 production and biofilm formation. Based on model that we built and confirmation from wetlab team, we found inoculation time until E.coli reaches quorum sensing condition is 10 hours.

Here ODEs that we used :

Growth curve

AI-2 Production

Biofilm Formation

Quorum Sensing

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Quorum Sensing

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