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| − | <div class="column full_size" > | + | <h1>Project Description</h1> |
| − | <img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
| + | <p>One of Jalisco's most representative products is <strong>tequila </strong>, which is an alcoholic beverage distilled from <i class="binomen">Agave tequilana </i> weber blue variety. Only 20% of the agave plant is used for tequila's manufacturing process; the rest, mainly the leaves, is disposed along with bagasse, which is the remaining fiber.</p> |
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| + | <p>The main goal of the project is to synthesize polyhydroxyalkanoates (PHA) from the available carbon sources in agave residues through a recombinant <i class="binomen">E. coli </i> strain.</p> |
| − | | + | <p>The project will proceed in two stages. The first one will consist on the degradation of the cellulose to glucose through two different methods in order to compare their effectiveness and efficiency. The first method consists on degrading the bagazo through the metabolism of <i class="binomen">Aspergillus niger</i> in controlled conditions; the residues of the agave for this stage will go through an acid and alkaline pretreatment , with H<sub>2</sub>SO<sub>4</sub> and NaOH respectively, in order to facilitate the access to the raw material for the <i class="binomen">A. niger </i>. The second method will consist in a protocol to treat through heat, chemicals and grinding of the bagasse in order to obtain approximately 89% of the held cellulose within the fibers.</ p> |
| − | | + | <p>The second stage will be a comparative study of the metabolic speed for the synthesis of PHAs between <i class="binomen">Pseudomona putida </i> and a recombinant <i class="binomen">E. coli </i> with the genes <i class="gene">phac1 </i> and <i class="gene">phac2 </i> for a continuous production of the bioplastic. Also, the already registered PHA production cluster <a href="http://parts.igem.org/Part:BBa_K1112002 ">Part:BBa_K1112002</a> will be used as a control group in the transformation of <i class="binomen">E. coli </i>. This stage will include the extraction of the PHAs from the intracellular medium. The <i class="binomen">E. coli </i> will be modified with the help of synthetic biology to include a gene to release the PHA to the outer medium, thus avoiding the breaking of the cellular membrane. The main objective is to make the process efficient and with a high yield.< /p> |
| − | | + | <p> The bioplastic obtained from this process has multiple applications; for example, it can be used for the creation of disposable medical equipment and containers because of their resistance to heat and oils. A business model for the commercialization of our bioplastic will be included.</p> |
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| − | <h1> Welcome to iGEM 2017! </h1> | + | |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
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| − | <h5>Before you start: </h5>
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| − | <p> Please read the following pages:</p>
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| − | <ul>
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| − | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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| − | </ul>
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| − | <h5> Project Description </h5>
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| − | <p> One of Jalisco’s most representative products is tequila, which is an alcoholic beverage distilled from Agave tequilana weber blue variety. Only 20% of the agave plant is used for tequila’s manufacturing process; the rest, mainly the leaves, is disposed along with bagasse, which is the remaining fiber. </p> | + | |
| − | <p> The main goal of the project is to synthesize polyhydroxyalkanoates (PHA) from the available carbon sources in agave residues through a recombinant E. coli strain. The bioplastic obtained from this process has multiple applications; for example, it can be used for the creation of disposable medical equipment and containers because of their resistance to heat and oils. A business model for the commercialization of our bioplastic is included. </p> | + | |
| − | <p> The project will proceed in two stages. The first one will consist on the degradation of the cellulose to glucose. In order to access the cellulose within the organic tissue of the bagasse and leaves of the agave a chemical and physical pretreatment will expose the cellulose to a culture of Aspergillus niger. The A. niger will then metabolize the cellulose into glucose. <p/> | + | |
| − | <p> The second stage will be a comparative study of the metabolic speed for the synthesis of PHAs between Pseudomona putida and a recombinant E. coli with the genes phac1 and phac2 for a continuous production of the bioplastic. Also, the already registered PHA production cluster Part:BBa_K1112002 will be used as a control group in the transformation of E.coli. This stage will include the extraction of the PHAs from the intracellular medium. The E. coli will be modified with the help of synthetic biology to include a gene to release the PHA to the outer medium, thus avoiding the breaking of the cellular membrane. The main objective is to make the process efficient and with a high yield. <p/> | + | |
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| − | <h5> Wiki template information </h5>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p> | + | |
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h5>Inspiration</h5>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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| − | <a href="https://2017.igem.org/Special:Upload">
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| − | UPLOAD FILES
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Project Description
One of Jalisco's most representative products is tequila, which is an alcoholic beverage distilled from Agave tequilana weber blue variety. Only 20% of the agave plant is used for tequila's manufacturing process; the rest, mainly the leaves, is disposed along with bagasse, which is the remaining fiber.
The main goal of the project is to synthesize polyhydroxyalkanoates (PHA) from the available carbon sources in agave residues through a recombinant E. coli strain.
The project will proceed in two stages. The first one will consist on the degradation of the cellulose to glucose through two different methods in order to compare their effectiveness and efficiency. The first method consists on degrading the bagazo through the metabolism of Aspergillus niger in controlled conditions; the residues of the agave for this stage will go through an acid and alkaline pretreatment, with H2SO4 and NaOH respectively, in order to facilitate the access to the raw material for the A. niger. The second method will consist in a protocol to treat through heat, chemicals and grinding of the bagasse in order to obtain approximately 89% of the held cellulose within the fibers.
The second stage will be a comparative study of the metabolic speed for the synthesis of PHAs between Pseudomona putida and a recombinant E. coli with the genes phac1 and phac2 for a continuous production of the bioplastic. Also, the already registered PHA production cluster Part:BBa_K1112002 will be used as a control group in the transformation of E. coli. This stage will include the extraction of the PHAs from the intracellular medium. The E. coli will be modified with the help of synthetic biology to include a gene to release the PHA to the outer medium, thus avoiding the breaking of the cellular membrane. The main objective is to make the process efficient and with a high yield.
The bioplastic obtained from this process has multiple applications; for example, it can be used for the creation of disposable medical equipment and containers because of their resistance to heat and oils. A business model for the commercialization of our bioplastic will be included.
