Difference between revisions of "Team:MSU-Michigan"
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| − | <h1> Welcome to iGEM | + | <h1> Welcome to Michigan State University's 2017 iGEM wiki! </h1> |
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| − | < | + | <h3>Abstract </h3> |
| − | <p> | + | <h4> <b> Biosensing Water Contaminants with Genetically Engineered <em>Shewanella oneidensis MR-1 </em> </b></h6> |
| + | <p> As one of the most important natural resources, the declining quality of fresh water is a world-wide issue. Pollutants such as pharmaceuticals, hormones and heavy metals are rarely monitored and the need to detect and remove these compounds in an inexpensive way is what motivates this project. The marine bacterium Shewanella oneidensis MR-1 could be a part of the solution through its unique ability to interact with electronics. The Mtr pathway in S. oneidensis MR-1 is an external electron transportation pathway that is able to transfer electrons to an external acceptor such as an anode, thus generating electric current that is utilized in bioelectrochemical systems. We are utilizing S. oneidensis MR-1 △MtrB, the MtrB gene is removed, to prevent electron flow to an outside source through this key protein. We are then inserting a plasmid into this strain that contains the MtrB gene under control of a promoter that activates transcription of the protein when induced by compounds such as pesticides and metals in the water. This will allow for controlled electrical expression that can be used in microbial fuel cells to create a biosensor for the detection of these compounds. This biosensor will then be engineered to be manufactured on a large scale to be used for research, education, humanitarian efforts and even consumer use. Although the proof of concept is currently tested in a single chambered bioelectrochemical system, an affordable and portable paper microbial fuel cell system is currently in development. | ||
| + | </p> | ||
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<li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li> | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li> | ||
Revision as of 19:17, 28 June 2017
Follow Shewlock Holmes on his 10 Week Search!
Welcome to Michigan State University's 2017 iGEM wiki!
Abstract
Biosensing Water Contaminants with Genetically Engineered Shewanella oneidensis MR-1
As one of the most important natural resources, the declining quality of fresh water is a world-wide issue. Pollutants such as pharmaceuticals, hormones and heavy metals are rarely monitored and the need to detect and remove these compounds in an inexpensive way is what motivates this project. The marine bacterium Shewanella oneidensis MR-1 could be a part of the solution through its unique ability to interact with electronics. The Mtr pathway in S. oneidensis MR-1 is an external electron transportation pathway that is able to transfer electrons to an external acceptor such as an anode, thus generating electric current that is utilized in bioelectrochemical systems. We are utilizing S. oneidensis MR-1 △MtrB, the MtrB gene is removed, to prevent electron flow to an outside source through this key protein. We are then inserting a plasmid into this strain that contains the MtrB gene under control of a promoter that activates transcription of the protein when induced by compounds such as pesticides and metals in the water. This will allow for controlled electrical expression that can be used in microbial fuel cells to create a biosensor for the detection of these compounds. This biosensor will then be engineered to be manufactured on a large scale to be used for research, education, humanitarian efforts and even consumer use. Although the proof of concept is currently tested in a single chambered bioelectrochemical system, an affordable and portable paper microbial fuel cell system is currently in development.
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2017 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
UPLOAD FILES
