Difference between revisions of "Team:BostonU HW/Digestion"

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<title>Digestion</title>
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<title>Cell Sorting</title>
  
 
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
 
<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
 
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
 
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<img src="https://static.igem.org/mediawiki/2017/7/7a/MARS_Digestion.png" id="TITLE">
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<img src="https://static.igem.org/mediawiki/2017/e/ee/MARS_CellSorting.png" id="TITLE">
 
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<h1>Summary</h1>
 
<h1>Summary</h1>
 
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DNA digestion, or DNA fragmentation, is a basic protocol in synthetic biology. This is typically performed prior to analysis of the DNA sequence, or in order to perform further protocols. Restriction enzymes are mixed and then incubated with DNA in a buffer solution. This yields DNA fragments cleaved at specific sites according to the enzymes used.
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The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
 
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<a href="https://static.igem.org/mediawiki/2017/2/27/MARS_Digestion_Protocol.pdf"download>
 
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This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipette.
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This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.
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                                <br><br>
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This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
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This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
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<a href="https://static.igem.org/mediawiki/2017/d/d2/MARS_CS_Protocol.pdf"download>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/6/69/MARS_CellSorting_F.png" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/thumb/7/7f/MARS_CellSorting_MF.png/800px-MARS_CellSorting_MF.png.jpeg" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
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<h1>Testing</h1>
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This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
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No biological material was inserted into this chip.
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Revision as of 01:03, 31 October 2017

BostonU_HW

Cell Sorting

Summary

The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.

This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
Picture
Picture

Testing

This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip. No biological material was inserted into this chip.