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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | <img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | ||
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<h1>Summary</h1> | <h1>Summary</h1> | ||
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− | + | The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols. | |
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− | This microfluidic chip design | + | This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output. |
− | + | <br><br> | |
− | This chip has been milled and | + | This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers. |
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+ | <div style="text-align:center; margin:auto; vertical-align:middle;" > | ||
+ | <a href="https://static.igem.org/mediawiki/2017/d/d2/MARS_CS_Protocol.pdf"download> | ||
+ | <button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button> | ||
+ | </a> | ||
+ | </div> | ||
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− | <button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button> | + | <button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button> |
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− | + | <button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button> | |
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+ | <div class="col-md-6" style="margin-bottom:5%;"> | ||
+ | <video style="width:100%; margin-top:20px; padding-top:18px;" controls> | ||
+ | <source src="https://static.igem.org/mediawiki/2017/7/75/MARS_CellSortingVid.mp4" type="video/mp4"> | ||
+ | </video> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <h1>Testing</h1> | ||
+ | <div class="text"> | ||
+ | This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip. | ||
+ | No biological material was inserted into this chip. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
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Revision as of 01:03, 31 October 2017
![](https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png)
![](https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png)
![](https://static.igem.org/mediawiki/2017/e/ee/MARS_CellSorting.png)
Summary
The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.
This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
This chip has been milled, tested and deemed fully fluid functional. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
![Picture](https://static.igem.org/mediawiki/2017/6/69/MARS_CellSorting_F.png)
![Picture](https://static.igem.org/mediawiki/2017/thumb/7/7f/MARS_CellSorting_MF.png/800px-MARS_CellSorting_MF.png.jpeg)
Testing
This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
No biological material was inserted into this chip.