Difference between revisions of "Team:UNOTT/Experiments"

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<h1>Why</h1>
 
<p>For <i>Key. coli</i> to work as intended and not deteriorate we need need to things to occur:  
 
<p>For <i>Key. coli</i> to work as intended and not deteriorate we need need to things to occur:  
 
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<p>To accomplish this, we chose to freeze dry the cells within the key. This involved</p>
 
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Revision as of 20:34, 31 October 2017





EXPERIMENTS:

STEP 1: Create guideRNA Plasmid

STEP 2: Create Reporter Plasmid

STEP 3: Promoter Library

STEP 4: Random Ligations

STEP 5: Freeze Drying & Revival

STEP 6: CRISPRi & gRNA Efficiency