Difference between revisions of "Team:UNOTT/Experiments"
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<h1>How</h1> | <h1>How</h1> | ||
| − | <p>To accomplish this, we chose to freeze dry the cells within the key. Click <a href=""> here <a> for out protocol for freeze-drying cells.</p> | + | <p>To accomplish this, we chose to freeze dry the cells within the key. Click <a href=""> here </a> for out protocol for freeze-drying cells.</p> |
<h1>Results</h1> | <h1>Results</h1> | ||
<img src="https://static.igem.org/mediawiki/2017/4/40/T--UNOTT--SP4WP1RFP.png"> | <img src="https://static.igem.org/mediawiki/2017/4/40/T--UNOTT--SP4WP1RFP.png"> | ||
| − | <p class="imgcaption"><b>Figure 1:</b> Graph of strong promoter 4 and weak promoter 1 transformed cells RFP fluorescence assay after freeze-drying revival two weeks and three weeks after samples were freeze dried. </p> | + | <p class="imgcaption"><b>Figure 1:</b> Graph of strong promoter 4 and weak promoter 1 transformed cells RFP fluorescence assay after freeze-drying revival two weeks and three weeks after samples were freeze dried. </p> |
| + | <br> | ||
| + | <br> | ||
| + | <p>In our results for freeze-dried cell revival, seen in Figure 1, we can show that storage temperatures do not have an effect on the revival of cells. For strong promoter 4 (SP4), at timepoint of 4 hours and 6 hours the storage temperature does seem to have a negative affect on the relative RFP fluorescence. However our sample size is very small meaning further assays would be needed to confirm this.</p> | ||
<link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet"> | <link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet"> | ||
</div> | </div> | ||
Revision as of 21:24, 31 October 2017
EXPERIMENTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3: Promoter Library
STEP 4: Random Ligations
STEP 5: Freeze Drying & Revival
