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<div style="background: #e8e6d1; padding: 30px; color: #1c2922"> | <div style="background: #e8e6d1; padding: 30px; color: #1c2922"> | ||
<h1 class="ITB_h1" style="padding-bottom: 60px; margin-bottom: 10px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; font-size: 100px; text-align: center; color: #1c2922">WORKS!</h1> | <h1 class="ITB_h1" style="padding-bottom: 60px; margin-bottom: 10px; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; font-size: 100px; text-align: center; color: #1c2922">WORKS!</h1> | ||
− | <p style="font-style: ITB_h1;text-align: justify; color: #1c2922; margin-bottom: 50px;">All the basic molecular techniques in the lab is performed by the students in the team. We designed the synthetic gBlocks, | + | <p style="font-style: ITB_h1;text-align: justify; color: #1c2922; margin-bottom: 50px;">All the basic molecular techniques in the lab is performed by the students in the team. We designed the synthetic gBlocks, cloned them into vector, and performed the characterization of respective BioBricks. </p> |
− | < | + | <h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Project Idea</h1> |
<p>The idea is based on the previous <a href="https://2014.igem.org/Team:ITB_Indonesia" style="color:black"> ITB iGEM 2014 </a> project which is called ColiPlaster. The 2017 team developed the idea for the optimization and novel enzymes. <br> | <p>The idea is based on the previous <a href="https://2014.igem.org/Team:ITB_Indonesia" style="color:black"> ITB iGEM 2014 </a> project which is called ColiPlaster. The 2017 team developed the idea for the optimization and novel enzymes. <br> | ||
We also get many inspirations from:<br> | We also get many inspirations from:<br> | ||
• <a href="https://2012.igem.org/Team:University_College_London" style="color:black" > Plastic Republic </a> , <br> | • <a href="https://2012.igem.org/Team:University_College_London" style="color:black" > Plastic Republic </a> , <br> | ||
− | • <a href="https://2012.igem.org/Team:UC_Davis" style="color:black">Team UC Davis</a> , and <br> | + | • <a href="https://2012.igem.org/Team:UC_Davis" style="color:black">Team UC Davis</a>, and <br> |
• <a href="ttp://2016.igem.org/Team:BGU_ISRAEL" style="color:black">PlastiCure </a> <br> | • <a href="ttp://2016.igem.org/Team:BGU_ISRAEL" style="color:black">PlastiCure </a> <br> | ||
</p> | </p> | ||
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<p>The main enzyme used in this project <a href="http://www.uniprot.org/uniprot/A0A0K8P6T7" style="color:#1c2922"> PETase </a> is based on the research paper: <br> | <p>The main enzyme used in this project <a href="http://www.uniprot.org/uniprot/A0A0K8P6T7" style="color:#1c2922"> PETase </a> is based on the research paper: <br> | ||
− | Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y., Toyohara K., Miyamoto K., Kimura Y., Oda K. 2016. A bacterium that degrades and assimilates poly (ethylene terephthalate). Science 351:1196-1199</p> | + | <b>Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y., Toyohara K., Miyamoto K., Kimura Y., Oda K.</b> 2016. A bacterium that degrades and assimilates poly (ethylene terephthalate). <i>Science</i> 351:1196-1199</p> |
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<p> | <p> | ||
− | Azzania FIbriani, Ph. D as Head of Molecular and Chromosome Analysis Lab, School of Life Science and Technology, Institut Teknologi Bandung, who provided us lab spaces and permission to use the instruments inside the lab. | + | <b>Azzania FIbriani, Ph. D</b> as Head of Molecular and Chromosome Analysis Lab, School of Life Science and Technology, Institut Teknologi Bandung, who provided us lab spaces and permission to use the instruments inside the lab.<br><br> |
− | Tati Kristianti, M. Sc., as Research Assistant from Genetics and Molecular Biotechnology group SITH ITB, who assisted us in providing the kit, enzymes, and helped us in anything related to customs and online ordering. | + | <b>Tati Kristianti, M. Sc.,</b> as Research Assistant from Genetics and Molecular Biotechnology group SITH ITB, who assisted us in providing the kit, enzymes, and helped us in anything related to customs and online ordering.<br><br> |
− | Ika Agus Rini, M. Sc., our team mentor, who provide insights and experience on iGEM. | + | <b>Ika Agus Rini, M. Sc.,</b> our team mentor, who provide insights and experience on iGEM.<br><br> |
− | Rahmat Azhari Kemal, M. Sc., last year Wetlab coordinator, who has given us insights on first steps in starting the lab work. | + | <b>Rahmat Azhari Kemal, M. Sc.,</b> last year Wetlab coordinator, who has given us insights on first steps in starting the lab work.<br><br> |
− | Agus Eka Prasetyo, S. Si, who first gives us the idea and tutor on using Restriction-Free Cloning techniques in ligating our constructs. | + | <b>Agus Eka Prasetyo, S. Si,</b> who first gives us the idea and tutor on using Restriction-Free Cloning techniques in ligating our constructs.<br><br> |
</p> | </p> | ||
<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Modelling Support</h1> | <h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Modelling Support</h1> | ||
− | <p>Mochamad Apri, Ph. D., lecturer from Mathematics Study Program ITB, who has given the modelling team insightful analysis on constructing the model.</p> | + | <p><b>Mochamad Apri, Ph. D.,</b> lecturer from Mathematics Study Program ITB, who has given the modelling team insightful analysis on constructing the model.</p> |
<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Human Practice Support</h1> | <h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Human Practice Support</h1> | ||
− | <p>Sri Harjati Suhardi, Ph. D, lecturer from Microbial Biotechnology group, who has given us experienced insights on bioremediation, especially in Indonesia.</p> | + | <p><b>Sri Harjati Suhardi, Ph. D,</b> lecturer from Microbial Biotechnology group, who has given us experienced insights on bioremediation, especially in Indonesia.</p> |
<h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Our Sponsors | <h1 class="ITB_h1" style="padding-bottom: 30px; margin-bottom: 50px; font-size: 36pt; border-bottom: 2px solid #1c2922 !important; padding-left: 30px; text-align: center; color:#1c2922">Our Sponsors | ||
</h1> | </h1> |
Revision as of 15:41, 1 November 2017
Attributions
Acknowledgement
This project wouldn't be possible without commitment and dedication from many parties. It is our greatest pleasure to acknowledge the assistance and contribution of the individuals and groups who have helped us towards the successful completion of the project.
WORKS!
All the basic molecular techniques in the lab is performed by the students in the team. We designed the synthetic gBlocks, cloned them into vector, and performed the characterization of respective BioBricks.
Project Idea
The idea is based on the previous ITB iGEM 2014 project which is called ColiPlaster. The 2017 team developed the idea for the optimization and novel enzymes.
We also get many inspirations from:
• Plastic Republic ,
• Team UC Davis, and
• PlastiCure
Project References
The main enzyme used in this project PETase is based on the research paper:
Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y., Toyohara K., Miyamoto K., Kimura Y., Oda K. 2016. A bacterium that degrades and assimilates poly (ethylene terephthalate). Science 351:1196-1199
Wetlab Support
Azzania FIbriani, Ph. D as Head of Molecular and Chromosome Analysis Lab, School of Life Science and Technology, Institut Teknologi Bandung, who provided us lab spaces and permission to use the instruments inside the lab.
Tati Kristianti, M. Sc., as Research Assistant from Genetics and Molecular Biotechnology group SITH ITB, who assisted us in providing the kit, enzymes, and helped us in anything related to customs and online ordering.
Ika Agus Rini, M. Sc., our team mentor, who provide insights and experience on iGEM.
Rahmat Azhari Kemal, M. Sc., last year Wetlab coordinator, who has given us insights on first steps in starting the lab work.
Agus Eka Prasetyo, S. Si, who first gives us the idea and tutor on using Restriction-Free Cloning techniques in ligating our constructs.
Modelling Support
Mochamad Apri, Ph. D., lecturer from Mathematics Study Program ITB, who has given the modelling team insightful analysis on constructing the model.
Human Practice Support
Sri Harjati Suhardi, Ph. D, lecturer from Microbial Biotechnology group, who has given us experienced insights on bioremediation, especially in Indonesia.