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<h2>Microalgae Transformation Platform</h2> | <h2>Microalgae Transformation Platform</h2> | ||
− | <p>To transform pigments genes into <i>Synechococcus elongatus</i> PCC 7942, we created a well-designed platform | + | <p>To transform pigments genes into <i>Synechococcus elongatus</i> PCC 7942, we created a well-designed platform for microalgae transformation. |
</p> | </p> | ||
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<div class='right_column'> | <div class='right_column'> | ||
<h2>Constructs & Transformants</h2> | <h2>Constructs & Transformants</h2> | ||
− | <p>We sent | + | <p>We sent 13 parts to iGEM and successfully transformed pigments into <i>Synechococcus elongatus</i> PCC 7942.</p> |
<div id='s4' class='expandable' style='height:32px;'> | <div id='s4' class='expandable' style='height:32px;'> | ||
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<font class='h7'>Endolysin-Holin-NrtA</font> | <font class='h7'>Endolysin-Holin-NrtA</font> | ||
</a> | </a> | ||
− | <p style='padding-top:0px;'>We successfully transformed NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803 to <i>E.coli</i>. The engineering <i>E.coli</i> which | + | <p style='padding-top:0px;'>We successfully transformed NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803 to <i>E.coli</i>. The engineering <i>E.coli</i> which expresses NrtA protein can catch nitrate (NO<sub>3</sub><sup>-</sup>) and nitrite (NO<sub>2</sub><sup>-</sup>) to induce algae undergoing nitrogen starvation. Due to biosafety concern, we also improved a suicide mechanism endolysin-holin and successfully constructed it. Moreover, we even grouped endolysin-holin and NrtA into a powerful part. <a href='https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation'>(see more detail: Nitrogen Starvation - Functional Test)</a> |
</p> | </p> | ||
</div> | </div> | ||
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<div class='right_column'> | <div class='right_column'> | ||
<h2>Assistance to Establish New iGEM Teams</h2> | <h2>Assistance to Establish New iGEM Teams</h2> | ||
− | <p>We | + | <p>We helped students in Chung Hsing University and Taipei Wego Senior High School to establish their own iGEM teams. We are looking forward to seeing them at Boston in 2018! |
</p> | </p> | ||
</div> | </div> | ||
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<h2>Experimental Technology</h2> | <h2>Experimental Technology</h2> | ||
− | <p>In addition to our comprehensive microalgae transformation platform, to achieve our goals, we also applied lots of experimental | + | <p>In addition to our comprehensive microalgae transformation platform, to achieve our goals, we also applied lots of experimental technologies. Although most of our team members are freshman in college, we tried hard to learn relatively complicated experimental methods. Moreover, we realized that, with more experimental technologies and knowledge, we can be closer to our goals and do more things for the world. Therefore, we strongly encourage future iGEM teams to explore new technologies. Deeper thinking and thrive on challenge that come along with the journey of learning are as important as the technology itself. |
</p> | </p> | ||
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<p><b>French Pressure Cell Press NrtA</b><br> | <p><b>French Pressure Cell Press NrtA</b><br> | ||
− | We | + | We used French pressure cell press in NrtA functional test. |
</p> | </p> | ||
<p><b>Microalgae DNA Extraction</b><br> | <p><b>Microalgae DNA Extraction</b><br> | ||
− | We | + | We extracted our engineered <i>Synechococcus elongatus</i> PCC 7942 genomic DNA to confirm the correctness of transformation. |
</p> | </p> | ||
Latest revision as of 19:39, 1 November 2017
Contribution
Microalgae Transformation Platform
To transform pigments genes into Synechococcus elongatus PCC 7942, we created a well-designed platform for microalgae transformation.
Constructs & Transformants
We sent 13 parts to iGEM and successfully transformed pigments into Synechococcus elongatus PCC 7942.
Modeling
We established 13 models to check and predict the results of the experiments. These models are extremely important to our project! (see more detail: Modeling)
Unique and Detailed Protocols for the Public
We provided unique and detailed protocols of our experiments to the public. Students, teachers, scientists all over the world and future iGEM teams can repeat our experiments or design their own experiments with these protocols. (see more detail: Notebook - Protocols)
Sharing Genetic Engineering Technology
Assistance to Establish New iGEM Teams
We helped students in Chung Hsing University and Taipei Wego Senior High School to establish their own iGEM teams. We are looking forward to seeing them at Boston in 2018!
Experimental Technology
In addition to our comprehensive microalgae transformation platform, to achieve our goals, we also applied lots of experimental technologies. Although most of our team members are freshman in college, we tried hard to learn relatively complicated experimental methods. Moreover, we realized that, with more experimental technologies and knowledge, we can be closer to our goals and do more things for the world. Therefore, we strongly encourage future iGEM teams to explore new technologies. Deeper thinking and thrive on challenge that come along with the journey of learning are as important as the technology itself.
Fusion PCR
We used fusion PCR in NrtA construct, pPIGBACK construct and fusion of pigments and the promoter prbcL.
Sticky End PCR
We used sticky end PCR in holin, endolysin, NrtA and CrtZ construct.
3 Piece Overlap PCR
We used 3 piece overlap PCR in lycopene and pPIGBACK construct.
Site Directed Mutagenesis
We used site directed mutagenesis in pPIGBACK and PrbcL construct.
Electroporation
We used electroporation in IndC construct.
Making Competent Cell
We made competent cell in electroporation experiments.
French Pressure Cell Press NrtA
We used French pressure cell press in NrtA functional test.
Microalgae DNA Extraction
We extracted our engineered Synechococcus elongatus PCC 7942 genomic DNA to confirm the correctness of transformation.