Difference between revisions of "Team:REC-CHENNAI/Notebook"

Revision as of 17:42, 1 November 2017

February
15	Finalized team
15	Created a group on WhatsApp. After a lot of reading, we got an idea, something about Lactoferrin.
15-20	Reading reading Zzzz
20	Team met to understand the registration process.
21	Had a discussion and concluded that Lf is too big a protein to work with in this short time span. Dropped it.
22	Back to square one. No project idea. Split ourselves into 3 groups, each focusing on a different problem statement.
March & April
	Brainstorming and Fundraising.
May
02	Break for end semester exams.
03	Whole team met Ramit. He agreed to mentor our team.
10	Opened our iGEM kit.
10-21	Lab construction and renovation in progress. Meanwhile literature work , brainstorming for project ideas.
15-20	Decided to work with AMPs Came across papers related to Latarcin Decided to work with this particular peptide Lab construction and renovation in progress.
June
01-09	Brainstorming for applications. Lab construction and renovation in progress.
10	First skype session with Carolina. Finalized our project idea.
11	Team was split into wet lab, interlab study and HP team.
13	First team fight :P
13-25	Placed order for all chemicals and kits required.
26	Met Dr. N.G.Iboyaima Singh and Dr. G.R.Gopi to discuss our Delivery system.
27	Team meeting to discuss about mutant latarcin. Something seemed off. We realized that people were still not comfortable working with each other. Decided to talk it out like adults :P
28	Team bonding time. Played basketball. Went out for lunch.
29-30	Preparing buffers.
July
02	Competent cell preparation.
03	Transformation of plasmid from the iGEM kit.
05	No growth, Failed.
06-17	Alter protocol- prepare competent cells- transform- fail terribly- repeat.
15-16	Marcia & Nishka attended the All India iGEM Meet at IISER, Pune.
20	Got our first transformation results.
26	Conducted a workshop for school students.
27-30	Designed and ordered our gblocks.
August
01-12	Plasmid isolation and restriction digestion optimisation. Worked on improving our transformation efficiency.
13	Conducted Biofair with SVCE. Turned out pretty well despite the fluctuating weather.
14-30	Placed order for our primers. Started working on the wiki. Worked on our questionnaire for the HP.
September
02	Ligation refused to work out. Decided to move on to interlab study.
04-20	Interlab study
21-24	Back to working on ligation in the lab.
25-26	US VISA interview.VISA approved!
27	Skype session with UCopenhagen about Podcasts.
28-30	Compilation of HP work.
October
03-10	Restriction-Ligation-Transformation.
11-12	Worked on Podcasts.
13-20	Realized that Jamboree Preparation was way overdue! Started working on our Poster and Presentation.
21-30	Presentation practice. Final touches on Wiki. Get ready for the Jamboree.
November
01-07	Study-Crib-Write semester exam-Cry.Repeat.

February

Finalized team

Created a group on WhatsApp. After a lot of reading, we got an idea, something about Lactoferrin.

15-20

Reading reading Zzzz

Team met to understand the registration process.

Had a discussion and concluded that Lf is too big a protein to work with in this short time span. Dropped it.

Back to square one. No project idea. Split ourselves into 3 groups, each focusing on a different problem statement.

March & April

Brainstorming and Fundraising.

May

Break for end semester exams.

Whole team met Ramit. He agreed to mentor our team.

Opened our iGEM kit.

10-21

Lab construction and renovation in progress.
Meanwhile literature work , brainstorming for project ideas.

15-20

Decided to work with AMPs
Came across papers related to Latarcin
Decided to work with this particular peptide
Lab construction and renovation in progress.

June

01-09

Brainstorming for applications.
Lab construction and renovation in progress.

First skype session with Carolina.
Finalized our project idea.

Team was split into wet lab, interlab study and HP team.

First team fight :P

13-25

Placed order for all chemicals and kits required.

Met Dr. N.G.Iboyaima Singh and Dr. G.R.Gopi to discuss our Delivery system.

Team meeting to discuss about mutant latarcin.
Something seemed off. We realized that people were still not comfortable working with each other. Decided to talk it out like adults :P

Team bonding time. Played basketball. Went out for lunch.

29-30

Preparing buffers.

July

Competent cell preparation.

Transformation of plasmid from the iGEM kit.

No growth, Failed.

06-17

Alter protocol- prepare competent cells- transform- fail terribly- repeat.

15-16

Marcia & Nishka attended the All India iGEM Meet at IISER, Pune.

Got our first transformation results.

Conducted a workshop for school students.

27-30

Designed and ordered our gblocks.

August

01-12

Plasmid isolation and restriction digestion optimisation.
Worked on improving our transformation efficiency.

Conducted Biofair with SVCE. Turned out pretty well despite the fluctuating weather.

14-30

Placed order for our primers.
Started working on the wiki.
Worked on our questionnaire for the HP.

September

Ligation refused to work out.
Decided to move on to interlab study.

04-20

Interlab study

21-24

Back to working on ligation in the lab.

25-26

US VISA interview.VISA approved!

Skype session with UCopenhagen about Podcasts.

28-30

Compilation of HP work.

October

03-10

Restriction-Ligation-Transformation.

11-12

Worked on Podcasts.

13-20

Realized that Jamboree Preparation was way overdue!
Started working on our Poster and Presentation.

21-30

Presentation practice.
Final touches on Wiki.
Get ready for the Jamboree.

November

01-07

Study-Crib-Write semester exam-Cry.Repeat.

@@ Line 75: / Line 75: @@
 						<tr>
 							<td><i class="material-icons">event</i> 10</td>
-							<td><i class="material-icons">lightbulb_outline</i> Opened our iGEM kit</td>
+							<td><i class="material-icons">lightbulb_outline</i> Opened our iGEM kit.</td>
 						</tr>
 						<tr>
@@ Line 94: / Line 94: @@
 						<tr>
 							<td><i class="material-icons">event</i>10</td>
-							<td><i class="material-icons">lightbulb_outline</i>First skype session with Carolina.<br><i class="material-icons">lightbulb_outline</i> Finalized our project idea</td>
+							<td><i class="material-icons">lightbulb_outline</i>First skype session with Carolina.<br><i class="material-icons">lightbulb_outline</i> Finalized our project idea.</td>
 						</tr>
 						<tr>