- We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in S. cerevisiae.
- By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents
- With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.
- Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
- By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
- We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
- With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
- To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
- For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
Difference between revisions of "Team:Cologne-Duesseldorf/Test4"
| Line 28: | Line 28: | ||
<article> | <article> | ||
<ul class="eric"> | <ul class="eric"> | ||
| − | + | <li>We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae</i>.</li> | |
| + | <li>By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents </li> | ||
| + | <li>With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.</li> | ||
| + | <li>Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.</li> | ||
| + | <li>By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox</li> | ||
| + | <li>We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.</li> | ||
| + | <li>With a high throughput assay we characterized the import efficiency of different PTS2 sequences.</li> | ||
| + | <li>To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.</li> | ||
| + | <li>For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.</li> | ||
| + | </ul> | ||
| + | |||
</ul> | </ul> | ||
</article> | </article> | ||
Revision as of 19:57, 1 November 2017
