Difference between revisions of "Team:Georgia State/Contribution"

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<h1>Contribution</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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<h2><b>Georgia State 2017 Measurement Study</b></h2>
 
<h2><b>Georgia State 2017 Measurement Study</b></h2>
  
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<ul><table><tr><td><img src="https://static.igem.org/mediawiki/parts/b/bc/T--Georgia_State--BBa_J364005_Georgia_State_2017.png" alt=""></td></table>
 
<ul><table><tr><td><img src="https://static.igem.org/mediawiki/parts/b/bc/T--Georgia_State--BBa_J364005_Georgia_State_2017.png" alt=""></td></table>
 
<ul><table><tr><td>Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364005 in multiple growth mediums. </td></table>
 
<ul><table><tr><td>Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364005 in multiple growth mediums. </td></table>
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Revision as of 03:07, 2 November 2017

Georgia State 2017 Measurement Study

This year we decided to conduct a large-scale measurement study to ascertain an optimal growth medium for high protein yield in ''Escherichia coli''. The study utilized the following parts transformed into Neb5-Alpha competent cells:
  • BBa_I20270
  • BBa_R0040
  • BBa_J364000
  • BBa_J364001
  • BBa_J364002
  • BBa_J364003
  • BBa_J364004
  • BBa_J364005

    • Liquid Media

    The experiment included four types of liquid media which varied in nutritional complexity.
    1. 2x YT Broth
    2. LB Broth
    3. Terrific Broth
    4. Super Rich Broth
    Once transformed, the controls and test devices were cultured according to the 2017 Interlab protocol. Each media trial consisted of two replicates of each device and control. After six hours of monitored growth, the mean fluorescence intensities (MFI) of all the samples were measured with a BDFACScanto flow cytometer. Finally, the MFIs for each device were compiled and compared with FlowJo software by media type.

    Results

        Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_I20270 in multiple growth mediums.
            Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_R0040 in multiple growth mediums.
            Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa R0040 in multiple growth mediums.
                Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364000 in multiple growth mediums.
                    Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364001 in multiple growth mediums.
                        Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364002 in multiple growth mediums.
                            Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364003 in multiple growth mediums.
                                Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364004 in multiple growth mediums.
                                    Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364005 in multiple growth mediums.