Difference between revisions of "Team:NYMU-Taipei/Safety"
| Line 1: | Line 1: | ||
| + | {{NYMU-Taipei}} | ||
| + | {{NYMU-Taipei-css}} | ||
| + | |||
<html> | <html> | ||
| − | < | + | <head> |
| − | + | </head> | |
| − | + | ||
| − | + | <!-- start of content --> | |
| − | + | <body> | |
| − | + | <div class = "igem_2017_content_wrapper"> | |
| − | + | <h3></h3> | |
| − | + | <center> | |
| − | + | <img src="https://static.igem.org/mediawiki/2017/7/71/T--NYMU-Taipei--safety_title.jpg" | |
| − | + | style="width:45%;border-radius:50%;border:8px solid #412a07;"> | |
| − | + | </center> | |
| − | + | <h3></h3> | |
| − | + | <p></p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | < | + | |
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | / | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | <h1>Overview</h1> | |
| − | + | <blockquote> | |
| − | + | <p> Our team is deeply convinced that all biological experiments carry some risk to the environment and experimenter. Under the guideline of IGEM Headquarter, we strictly follow a high standard of biosafety and responsible biological engineering. We also take note of the Laboratory biosafety manual published by WHO to ensure our lab work conducting in compliance with safety regulation. Moreover, before conducting experiments in the lab, all team members have undergone lab training by our PI and team members in NYMU Taipei 2016. The training included laboratory emergency response procedures, operation protocols for all kinds of machines, and biological waste decontamination and disposal protocols. As one of the members of the synthetic biology community, we are responsible for abiding by the common rule.</p> | |
| − | + | </blockquote> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | <h1>Biosafety</h1> | |
| − | + | <blockquote> | |
| − | + | <h3>Organisms We Used</h3> | |
| − | + | <p> We use cyanobacteria <i>Synechocystis</i> sp. PCC 6803, cyanobacteria <i>Synechocystis</i> sp. PCC 7942 and <i>E.coli DH5α</i> in our project. We extract NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803. Next, we transform NrtA gene into <i>E.coli</i>. Finally, we co-culture the engineered <i>E.coli</i> with microalgae to induce the nitrogen starvation. Nitrogen starvation will enlarge the oil production of algae. After extracting oil from microalgae, we need to kill <i>E.coli</i> to prevent contamination and prevent the danger of spreading engineered <i>E.coli</i>. To deal with the safety of <i>E.coli</i>, we construct a suicide mechanism.</p> | |
| − | + | <h3>Strain information of <i>E.coli</i></h3> | |
| − | + | <p> According to the Globally Harmonized System (GHS), <i>E.coli DH5α</i> is not a dangerous substance. Base on Centers for Disease Control and Prevention (CDC) Guidelines (Biosafety in Microbiological and Biomedical Laboratories, 5th Edition<sup>1</sup>), <i>E.coli DH5α</i> is Biological Safety Level One organism.</p> | |
| − | + | <h3>Strain information of <i>PCC 6803</i> and <i>PCC 7942</i></h3> | |
| − | + | <p> <i>PCC 6803</i> and <i>PCC 7942</i> are the most widely used model organisms for photosynthesis research<sup>2, 3</sub>. Both belong to the biosafety level one organism.</p> | |
| − | + | <h3>Suicide Mechanism</h3> | |
| − | + | <p> Being afraid of causing environmental danger, we design a suicide mechanism, with the aim of killing <i>E.coli</i>. We design suicide genes, <b>endolysin</b> and <b>holing</b> to clear away all of engineered <i>E.coli</i>, ensuring the safety. Endolysin and holin we use are similar to the mechanism used by team Pecking (2014 iGEM Beijing). Holin can induce holes on cell membrane. Endolysin can pass through the membrane and decompose peptidoglycan if holin successfully triggers holes on cell membrane. After the cell membrane and cell wall are destroyed, <i>E.coli</i> will be lysed.</p> | |
| − | + | </blockquote> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </ | + | |
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </ | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </ | + | |
| − | + | ||
| − | + | ||
| + | <h1>Lab safety</h1> | ||
| + | <blockquote> | ||
| + | <p> All experiments were done in Biosafety Level 1 (BSL1) laboratory. Our lab is equipped with safety facilities, such as emergency shower and fire extinguishers, and we conduct experiments under Laboratory Safe Hygiene Precautions of our school.<sup>4</sup> To always remind us the importance of safety, the safety insert and postcard are hung at where can be seen easily in the lab. Furthermore, all experiment protocols were checked and guided by our PI, Dr. Chou before we conducted. </p> | ||
| + | <h3>Lab training</h3> | ||
| + | <p> Every members of wet lab in NYMU iGEM team had finished lab safety lesson and training twice, which are directed by professors who instructed us step by step. Thus, members could conduct experiments properly and safely.</p> | ||
| + | <h3>Protection</h3> | ||
| + | <p> In the laboratory, each member wears laboratory coat and gloves with long pants and shoes which cover feet entirely. Before going out of the experimental zone, we take off the coat and gloves, and wash our hands with antibacterial hand wash.</p> | ||
| + | <h3>Waste</h3> | ||
| + | <p> After experiments, we gather chemical and bacteria mixture into a bottle and add bleach. Besides, we sterilize trash with autoclave and put it at the specific area for contaminative trash. All the wastes in our lab are handled seriously and strictly to prevent contamination.</p> | ||
| + | </blockquote> | ||
| − | + | <h1>Reference</h1> | |
| − | < | + | <ol> |
| − | + | <li>U.S. Department of Health and Human Services. (2009). <i>Biosafety in Microbiological and Biomedical Laboratories</i> (5th ed.). HHS Publication No. (CDC) 21-1112 | |
| − | < | + | <br>(<a href='https://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf'>https://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf</a>) |
| − | + | <li> | |
| − | + | <br><a href=''></a> | |
| − | + | <li> | |
| − | + | <br><a href=''></a> | |
| − | < | + | <li> |
| − | + | <br><a href=''></a> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | + | ||
| − | + | </body> | |
| − | + | ||
| − | + | </html> | |
| − | </ | + | |
Revision as of 13:12, 1 October 2017
Overview
Our team is deeply convinced that all biological experiments carry some risk to the environment and experimenter. Under the guideline of IGEM Headquarter, we strictly follow a high standard of biosafety and responsible biological engineering. We also take note of the Laboratory biosafety manual published by WHO to ensure our lab work conducting in compliance with safety regulation. Moreover, before conducting experiments in the lab, all team members have undergone lab training by our PI and team members in NYMU Taipei 2016. The training included laboratory emergency response procedures, operation protocols for all kinds of machines, and biological waste decontamination and disposal protocols. As one of the members of the synthetic biology community, we are responsible for abiding by the common rule.
Biosafety
Organisms We Used
We use cyanobacteria Synechocystis sp. PCC 6803, cyanobacteria Synechocystis sp. PCC 7942 and E.coli DH5α in our project. We extract NrtA gene from cyanobacteria Synechocystis sp. PCC 6803. Next, we transform NrtA gene into E.coli. Finally, we co-culture the engineered E.coli with microalgae to induce the nitrogen starvation. Nitrogen starvation will enlarge the oil production of algae. After extracting oil from microalgae, we need to kill E.coli to prevent contamination and prevent the danger of spreading engineered E.coli. To deal with the safety of E.coli, we construct a suicide mechanism.
Strain information of E.coli
According to the Globally Harmonized System (GHS), E.coli DH5α is not a dangerous substance. Base on Centers for Disease Control and Prevention (CDC) Guidelines (Biosafety in Microbiological and Biomedical Laboratories, 5th Edition1), E.coli DH5α is Biological Safety Level One organism.
Strain information of PCC 6803 and PCC 7942
PCC 6803 and PCC 7942 are the most widely used model organisms for photosynthesis research2, 3. Both belong to the biosafety level one organism.
Suicide Mechanism
Being afraid of causing environmental danger, we design a suicide mechanism, with the aim of killing E.coli. We design suicide genes, endolysin and holing to clear away all of engineered E.coli, ensuring the safety. Endolysin and holin we use are similar to the mechanism used by team Pecking (2014 iGEM Beijing). Holin can induce holes on cell membrane. Endolysin can pass through the membrane and decompose peptidoglycan if holin successfully triggers holes on cell membrane. After the cell membrane and cell wall are destroyed, E.coli will be lysed.
Lab safety
All experiments were done in Biosafety Level 1 (BSL1) laboratory. Our lab is equipped with safety facilities, such as emergency shower and fire extinguishers, and we conduct experiments under Laboratory Safe Hygiene Precautions of our school.4 To always remind us the importance of safety, the safety insert and postcard are hung at where can be seen easily in the lab. Furthermore, all experiment protocols were checked and guided by our PI, Dr. Chou before we conducted.
Lab training
Every members of wet lab in NYMU iGEM team had finished lab safety lesson and training twice, which are directed by professors who instructed us step by step. Thus, members could conduct experiments properly and safely.
Protection
In the laboratory, each member wears laboratory coat and gloves with long pants and shoes which cover feet entirely. Before going out of the experimental zone, we take off the coat and gloves, and wash our hands with antibacterial hand wash.
Waste
After experiments, we gather chemical and bacteria mixture into a bottle and add bleach. Besides, we sterilize trash with autoclave and put it at the specific area for contaminative trash. All the wastes in our lab are handled seriously and strictly to prevent contamination.
Reference
- U.S. Department of Health and Human Services. (2009). Biosafety in Microbiological and Biomedical Laboratories (5th ed.). HHS Publication No. (CDC) 21-1112
(https://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf) -
-
-
