Difference between revisions of "Team:TU Darmstadt/project/chitin synthase"

Line 50: Line 50:
 
         <div class="container">
 
         <div class="container">
 
<header class="major">
 
<header class="major">
−
<h2>The Project: ChiTUcare</h2><!--BIG HEADING-->
+
<h2>Chitin Synthase NodC</h2><!--BIG HEADING-->
 
</header>
 
</header>
−
<p>Abstract: Faucibus sed lobortis aliquam lorem blandit. Lorem eu nunc metus col. Commodo id in arcu ante lorem ipsum sed accumsan erat praesent faucibus commodo ac mi lacus. Adipiscing mi ac commodo. Vis aliquet tortor ultricies non ante erat nunc integer eu ante ornare amet commetus vestibulum blandit integer in curae ac faucibus integer non. Adipiscing cubilia elementum.</p>
+
<p>Abstract:The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this waste, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides [Samain et al., 1997] of certain lengths which can be directly further processed in vivo.</p>
 
</div>
 
</div>
 
</section>
 
</section>

Revision as of 18:22, 13 October 2017

MainPage

Chitin Synthase NodC

Abstract:The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this waste, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides [Samain et al., 1997] of certain lengths which can be directly further processed in vivo.

Submenu 1 id "two"

Abstract: Faucibus sed lobortis aliquam lorem blandit. Lorem eu nunc metus col. Commodo id in arcu ante lorem ipsum sed accumsan erat praesent faucibus commodo ac mi lacus. Adipiscing mi ac commodo. Vis aliquet tortor ultricies non ante erat nunc integer eu ante ornare amet commetus vestibulum blandit integer in curae ac faucibus integer non. Adipiscing cubilia elementum.