Difference between revisions of "Team:TU Darmstadt/project/chitin synthase"

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<h2>Chitin Synthase NodC</h2><!--BIG HEADING-->
 
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<p>Abstract:The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this waste, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides [Samain et al., 1997] of certain lengths which can be directly further processed in vivo.</p>
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<h3>Abstract</h3>
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<p>The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this waste, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides [Samain et al., 1997] of certain lengths which can be directly further processed in vivo.</p>
 
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Revision as of 18:23, 13 October 2017

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Chitin Synthase NodC

Abstract

The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this waste, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides [Samain et al., 1997] of certain lengths which can be directly further processed in vivo.

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