Difference between revisions of "Team:USTC/Composite Part"

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                                                     <td>Coding</td>
 
                                                     <td>Coding</td>
 
                                                     <td>2326</td>
 
                                                     <td>2326</td>
−
                                                     <td>Its full name is Cytochrome c Maturation, which can mature Mtr CAB and enable it to function as we expected.</td>
+
                                                     <td>We use a promoter pTAC to control the gene CmCR’s expression, which is induced by IPTG.</td>
−
                                                     <td>We cloned this gene ccm A-H from the genome of an E.coli strain BL21(DE3).</td>
+
                                                    <td>The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene CmCR is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td>The substrate of this reductase is COBE, which is a organic compound with pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.</td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/PartBBa_K2242018">BBa_K2242018</a></td>
 +
                                                    <td>T7+LacO+Mtr</td>
 +
                                                    <td>Coding</td>
 +
                                                    <td>5272</td>
 +
                                                     <td>We use T7 promoter and Lac operator to control Mtr CAB’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.</td>
 +
                                                    <td>The plasmid is bought from a bio-company. The Mtr gene is obtained from the kit plate. The recombinant plasmid is constructed by ourselves.</td>
 
                                                     <td></td>
 
                                                     <td></td>
 
                                                   </tr>
 
                                                   </tr>
 
                                                   <tr>
 
                                                   <tr>
−
                                                     <td><a href="http://parts.igem.org/PartBBa_K2242233">BBa_K2242233</a></td>
+
                                                     <td><a href="http://parts.igem.org/PartBBa_K2242419">BBa_K2242419</td>
−
                                                     <td>CysDes</td>
+
                                                     <td>pTET+ccm</td>
 
                                                     <td>Coding</td>
 
                                                     <td>Coding</td>
−
                                                     <td>1203</td>
+
                                                     <td>6372</td>
−
                                                     <td>Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd<sup>2+</sup></td>
+
                                                     <td>We use a constitutively on promoter pTET to control gene ccmA-H’s expression.</td>
−
                                                     <td>Synthesized by IDT.</td>
+
                                                    <td>The plasmid backbone and the promoter pTET is from the kit plate. The ccm A-H gene is amplified from the genome of a E.coli strain BL21(DE3). The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td></td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/PartBBa_K2242633">BBa_K2242633</a></td>
 +
                                                    <td>pLuxR+CysDes</td>
 +
                                                    <td>Coding</td>
 +
                                                     <td>2367</td>
 +
                                                    <td>We use the pLuxR promoter from the quorum sensing system, which can be induced by AHL.</td>
 +
                                                    <td>The plasmid backbone and the promoter pLuxR is from the kit plate. The CysDes gene is synthesized by IDT. The recombinant plasmid is constructed by ourselves.</td>
 
                                                     <td>As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S<sup>2-</sup>is in the system, special measures must be taken.</td>
 
                                                     <td>As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S<sup>2-</sup>is in the system, special measures must be taken.</td>
 
                                                   </tr>
 
                                                   </tr>
 
                                                   <tr>
 
                                                   <tr>
−
                                                     <td><a href="http://parts.igem.org/PartBBa_K2242180">BBa_K2242180</td>
+
                                                     <td><a href="http://parts.igem.org/PartBBa_K2242005">BBa_K2242005</a></td>
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                                                     <td>Reductase CmCR</td>
+
                                                     <td>T7+LacO+KmAdh</td>
 
                                                     <td>Coding</td>
 
                                                     <td>Coding</td>
−
                                                     <td>873</td>
+
                                                     <td>948</td>
−
                                                     <td>This reductase CmCR is a NADH-dependent reductase which can catalyze the bio-transformation from COBE to (S)-CHBE.</td>
+
                                                     <td>We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.</td>
−
                                                     <td>Kindly provide by Prof.Hong Jiong</td>
+
                                                     <td>The plasmid is bought from a bio-company. The Mtr gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.</td>
−
                                                     <td>As COBE is harmful to our skin and respiratory tract with pungent smell, special measures must be taken.</td>
+
                                                     <td></td>
 
                                                   </tr>
 
                                                   </tr>
 
                                                   <tr>
 
                                                   <tr>
−
                                                     <td><a href="http://parts.igem.org/PartBBa_K2242626">BBa_K2242626</a></td>
+
                                                     <td><a href="http://parts.igem.org/PartBBa_K2242384">BBa_K2242384</a></td>
−
                                                     <td>Reductase KmAdh</td>
+
                                                     <td>placI+lacI+pTAC+KmAdh</td>
 
                                                     <td>Coding</td>
 
                                                     <td>Coding</td>
−
                                                     <td>888</td>
+
                                                     <td>2341</td>
−
                                                     <td>KmAdh is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature.</td>
+
                                                     <td>To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control it’s expression, which is induced by IPTG.</td>
−
                                                     <td>Kindly provide by Prof.Hong Jiong</td>
+
                                                     <td>The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene KMADH is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.</td>
 
                                                     <td></td>
 
                                                     <td></td>
 
                                                   </tr>
 
                                                   </tr>

Revision as of 07:15, 24 October 2017

子网页测试-队员


This year we, team USTC, submitted 5 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. Among these five basic parts, two of them are new, including reductase CmCR and KmAdh.

Part’s Number Part’s Name Type Length Short Description Source Safety Issues
BBa_K2242920 araC+pBAD+CmCR Coding 2089 To induce the gene expression, we use a promotor called pBAD, which is induced by arabinose. The plasmid is bought from a bio-company. The reductase CmCR’s gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves. The substrate of this reductase is COBE, which is a organic compound with pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242521 placI+lacI+pTAC+CmCR Coding 2326 We use a promoter pTAC to control the gene CmCR’s expression, which is induced by IPTG. The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene CmCR is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves. The substrate of this reductase is COBE, which is a organic compound with pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242018 T7+LacO+Mtr Coding 5272 We use T7 promoter and Lac operator to control Mtr CAB’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible. The plasmid is bought from a bio-company. The Mtr gene is obtained from the kit plate. The recombinant plasmid is constructed by ourselves.
BBa_K2242419 pTET+ccm Coding 6372 We use a constitutively on promoter pTET to control gene ccmA-H’s expression. The plasmid backbone and the promoter pTET is from the kit plate. The ccm A-H gene is amplified from the genome of a E.coli strain BL21(DE3). The recombinant plasmid is constructed by ourselves.
BBa_K2242633 pLuxR+CysDes Coding 2367 We use the pLuxR promoter from the quorum sensing system, which can be induced by AHL. The plasmid backbone and the promoter pLuxR is from the kit plate. The CysDes gene is synthesized by IDT. The recombinant plasmid is constructed by ourselves. As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S2-is in the system, special measures must be taken.
BBa_K2242005 T7+LacO+KmAdh Coding 948 We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible. The plasmid is bought from a bio-company. The Mtr gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.
BBa_K2242384 placI+lacI+pTAC+KmAdh Coding 2341 To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control it’s expression, which is induced by IPTG. The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene KMADH is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.
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