Difference between revisions of "Team:TU Darmstadt/Demonstrate"

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<h1>Demonstrate</h1>
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<header class="major">
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<h3>Gold Medal Criterion #4</h3>
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<h2>InterLab Measurement Studies</h2>
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<p>iGEM is coping with the problem of different groups measuring fluorescence differently, in different units and evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP.
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The study should be carried out and examined in different laboratories all over the world.
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Our team followed the standardized procedure guiding us through transformation, inoculation and measurement process. <br>For more information: <a href="https://2017.igem.org/Competition/InterLab_Study">Fourth International InterLab Measurement Study</a> <br>
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Following below you can find our results.
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</p>
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</div>
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</section>
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<section id="two"><div class="container">
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<h3>Calibration</h3>
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<h5>OD<sub>600</sub> reference point</h5><br>
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<p>Several measurements of LUDOX-S40 and H<sub style="font-size: 0.9em;">²</sub>O were spectrometric analyzed below.</p>
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<p><table style = "width:70%"; "HEIGHT:40%">
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                                                <thead>
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                                                  <tr>
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                                                    <td></td>
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                                                    <td>LUDOX-S40</td>
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                                                    <td>H<sub style="font-size: 0.9em;">²</sub>O</td>
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                                                  </tr>
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                                              </thead>
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                                                <tr>
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                                            <td>Replicate 1</td>
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                                                    <td>0,041700002</td>
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                                                    <td>0,035300002</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Replicate 2</td>
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                                                    <td>0,042399999</td>
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                                                    <td>0,035</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Replicate 3</td>
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                                                    <td>0,043000001</td>
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                                                    <td>0,035700001</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Replicate 4</td>
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                                                    <td>0,043000001</td>
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                                                    <td>0,034699999</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Arithmetic Mean</td>
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                                                    <td>0,042525001</td>
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                                                    <td>0,035175</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Corrected Absorbance 600</td>
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                                                    <td>0,007350001</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Reference OD<sub>600</sub> </td>
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                                                    <td>0,0425</td>
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                                                </tr>
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                                                <tr>
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                                                    <td>Correction Factor</td>
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                                                    <td>5,78231249</td>
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                                                </tr>
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                                            </table>
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<figcaption> Tab. 1: Hier fehlt was!!!. </figcaption></center>
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</p>
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<br>
 
<p>
 
<p>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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<figure><center>
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<img src="https://static.igem.org/mediawiki/2017/1/1b/T--TU_Darmstadt--Interlab1.gif", alt="hier fehlt was", width=50%,>
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<figcaption> Fig. 1: Hier fehlt was!!!. </figcaption></center>
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</figure>
 
</p>
 
</p>
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<br>
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<p>
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<h5>Fluorescein standard curve:</h5>
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<p>The fluorescence of a dilution series, fluorescein in four replicates were measured in standard modes in our Tecan plate reader (infinite 200Pro).</p>
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<br>
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<p>
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<figure><center>
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<img src="https://static.igem.org/mediawiki/2017/9/9f/T--TU_Darmstadt--Interlab2.gif", alt="hier fehlt was", width=100%,>
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<figcaption> Tab. 2: Hier fehlt was!!!. </figcaption></center>
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</figure></p><br>
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<p>Generated standard curve of fluorescence for different fluorescein concentrations can be found below.</p><br>
 
<p>
 
<p>
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<figure><center>
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<img src="https://static.igem.org/mediawiki/2017/6/63/T--TU_Darmstadt--Interlab3.gif", alt="hier fehlt was", width=50%,>
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<figcaption> Fig. 2: Hier fehlt was!!!. </figcaption></center>
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</figure>
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</p>
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<p>
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<figure><center>
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<img src="https://static.igem.org/mediawiki/2017/0/09/T--TU_Darmstadt--Interlab4.gif", alt="hier fehlt was", width=50%,>
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<figcaption> Fig. 3: Hier fehlt was!!!. </figcaption></center>
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</figure>
 
</p>
 
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<h3>Cell Measurement</h3>
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<h5>Fluorescence Measurement</h5>
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<h4> What should we do for our demonstration?</h4>
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<p>Text!!!</p>
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<p>
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<h5> Standard teams </h5>
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<figure><center>
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<img src="https://static.igem.org/mediawiki/2017/b/bd/T--TU_Darmstadt--Interlab5.gif", alt="hier fehlt was", width=70%,>
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<p>  
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<figcaption> Fig. 4: Hier fehlt was!!!. </figcaption></center>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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</figure>
 
</p>
 
</p>
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<div class="column half_size">
 
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<br>
 
<br>
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<h5> Special track teams </h5>
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<h5>Absorbance 600 Measurement</h5>
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<p>Text!!!</p>
 
<p>
 
<p>
−
Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
+
<figure><center>
 +
<img src="https://static.igem.org/mediawiki/2017/c/ca/T--TU_Darmstadt--Interlab6.gif", alt="hier fehlt was", width=70%,>
 +
<figcaption> Fig. 5: Hier fehlt was!!!. </figcaption></center>
 +
</figure>
 
</p>
 
</p>
 +
<br>
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<h5>Fluorescein / OD<sub>600</sub></h5>
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<p>Text!!!</p>
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<p>
 +
<figure><center>
 +
<img src="https://static.igem.org/mediawiki/2017/2/2a/7.7.png", alt="hier fehlt was", width=70%,>
 +
<figcaption> Fig. 6: Hier fehlt was!!!. </figcaption></center>
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</figure>
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</p>
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<br><br>
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<p>Abschlusssatz!!!</p>
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Revision as of 11:33, 26 October 2017

MainPage

InterLab Measurement Studies

iGEM is coping with the problem of different groups measuring fluorescence differently, in different units and evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP. The study should be carried out and examined in different laboratories all over the world. Our team followed the standardized procedure guiding us through transformation, inoculation and measurement process.
For more information: Fourth International InterLab Measurement Study
Following below you can find our results.

Calibration

OD600 reference point

Several measurements of LUDOX-S40 and H²O were spectrometric analyzed below.

LUDOX-S40 H²O
Replicate 1 0,041700002 0,035300002
Replicate 2 0,042399999 0,035
Replicate 3 0,043000001 0,035700001
Replicate 4 0,043000001 0,034699999
Arithmetic Mean 0,042525001 0,035175
Corrected Absorbance 600 0,007350001
Reference OD600 0,0425
Correction Factor 5,78231249
Tab. 1: Hier fehlt was!!!.


hier fehlt was
Fig. 1: Hier fehlt was!!!.


Fluorescein standard curve:

The fluorescence of a dilution series, fluorescein in four replicates were measured in standard modes in our Tecan plate reader (infinite 200Pro).


hier fehlt was
Tab. 2: Hier fehlt was!!!.


Generated standard curve of fluorescence for different fluorescein concentrations can be found below.


hier fehlt was
Fig. 2: Hier fehlt was!!!.

hier fehlt was
Fig. 3: Hier fehlt was!!!.

Cell Measurement

Fluorescence Measurement

Text!!!

hier fehlt was
Fig. 4: Hier fehlt was!!!.


Absorbance 600 Measurement

Text!!!

hier fehlt was
Fig. 5: Hier fehlt was!!!.


Fluorescein / OD600

Text!!!

hier fehlt was
Fig. 6: Hier fehlt was!!!.



Abschlusssatz!!!