Line 63: | Line 63: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368008">BBa_2368008</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368008">BBa_2368008</a> | ||
</td> | </td> | ||
− | <td>Gal 1/10-T1R3-ADH1</td> | + | <td><i>Gal 1/10-T1R3-ADH1</i></td> |
<td>Completed expression of the human source Sweet-receptor</td> | <td>Completed expression of the human source Sweet-receptor</td> | ||
<td>2814</td> | <td>2814</td> | ||
Line 71: | Line 71: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368023">BBa_2368023</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368023">BBa_2368023</a> | ||
</td> | </td> | ||
− | <td>MYC+T1R2 overlap</td> | + | <td><i>MYC+T1R2 overlap</i></td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>349</td> | <td>349</td> | ||
Line 79: | Line 79: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368009">BBa_2368009</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368009">BBa_2368009</a> | ||
</td> | </td> | ||
− | <td>HIS+T1R2 overlap</td> | + | <td><i>HIS+T1R2</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>50</td> | <td>50</td> | ||
Line 87: | Line 87: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368024">BBa_2368024</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368024">BBa_2368024</a> | ||
</td> | </td> | ||
− | <td>HIS+T1R3 overlap</td> | + | <td><i>HIS+T1R3</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>187</td> | <td>187</td> | ||
Line 95: | Line 95: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368011">BBa_2368011</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368011">BBa_2368011</a> | ||
</td> | </td> | ||
− | <td>FLAG+T1R3 overlap</td> | + | <td><i>FLAG+T1R3</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>50</td> | <td>50</td> | ||
Line 103: | Line 103: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368012">BBa_2368012</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368012">BBa_2368012</a> | ||
</td> | </td> | ||
− | <td>YCP+T1R2 overlap</td> | + | <td><i>YCP+T1R2</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>740</td> | <td>740</td> | ||
Line 112: | Line 112: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368013">BBa_2368013</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368013">BBa_2368013</a> | ||
</td> | </td> | ||
− | <td>YCP+T1R3 overlap</td> | + | <td><i>YCP+T1R3</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>740</td> | <td>740</td> | ||
Line 120: | Line 120: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368014">BBa_2368014</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368014">BBa_2368014</a> | ||
</td> | </td> | ||
− | <td>BCP+T1R2 overlap</td> | + | <td><i>BCP+T1R2</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>710</td> | <td>710</td> | ||
Line 128: | Line 128: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368015">BBa_2368015</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368015">BBa_2368015</a> | ||
</td> | </td> | ||
− | <td>GFP+T1R2 overlap</td> | + | <td><i>GFP+T1R2</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>699</td> | <td>699</td> | ||
Line 136: | Line 136: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368020">BBa_2368020</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368020">BBa_2368020</a> | ||
</td> | </td> | ||
− | <td>BCP+T1R3 overlap</td> | + | <td><i>BCP+T1R3</i> overlap</td> |
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
<td>710</td> | <td>710</td> | ||
Line 154: | Line 154: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368017">BBa_2368017</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368017">BBa_2368017</a> | ||
</td> | </td> | ||
− | <td>500bp Homologous | + | <td>500bp Homologous arm+<i>His</i></td> |
− | <td>knocking out the sst2 gene</td> | + | <td>knocking out the <i>sst2</i> gene</td> |
<td>2050</td> | <td>2050</td> | ||
</tr> | </tr> | ||
Line 162: | Line 162: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368018">BBa_2368018</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368018">BBa_2368018</a> | ||
</td> | </td> | ||
− | <td>500bp Homologous | + | <td>500bp Homologous arm+<i>Ura</i></td> |
− | <td>knocking out the far1 gene</td> | + | <td>knocking out the <i>far1</i> gene</td> |
<td>2050</td> | <td>2050</td> | ||
</tr> | </tr> | ||
Line 170: | Line 170: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368016">BBa_2368016</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368016">BBa_2368016</a> | ||
</td> | </td> | ||
− | <td>500bp Homologous | + | <td>500bp Homologous arm+<i>Trp</i></td> |
− | <td>knocking out the ste2 gene</td> | + | <td>knocking out the <i>ste2</i> gene</td> |
<td>2071</td> | <td>2071</td> | ||
</tr> | </tr> | ||
Line 179: | Line 179: | ||
</td> | </td> | ||
<td>Modified Gα Schizosaccharomyces pombe</td> | <td>Modified Gα Schizosaccharomyces pombe</td> | ||
− | <td>Signal | + | <td>Signal transduction</td> |
<td>1421</td> | <td>1421</td> | ||
</tr> | </tr> | ||
Line 187: | Line 187: | ||
</td> | </td> | ||
<td>Modified Gα Caenorhabditis elegans</td> | <td>Modified Gα Caenorhabditis elegans</td> | ||
− | <td>Signal | + | <td>Signal transduction</td> |
<td>1418</td> | <td>1418</td> | ||
</tr> | </tr> | ||
Line 195: | Line 195: | ||
</td> | </td> | ||
<td>Gα</td> | <td>Gα</td> | ||
− | <td>Signal | + | <td>Signal transduction</td> |
<td>1421</td> | <td>1421</td> | ||
</tr> | </tr> | ||
Line 210: | Line 210: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368005">BBa_2368005</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368005">BBa_2368005</a> | ||
</td> | </td> | ||
− | <td> | + | <td><i>P<sub>fus</sub></i></td> |
<td>Induced promoter</td> | <td>Induced promoter</td> | ||
<td>438</td> | <td>438</td> | ||
Line 218: | Line 218: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368026">BBa_2368026</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368026">BBa_2368026</a> | ||
</td> | </td> | ||
− | <td> | + | <td><i>P<sub>fus</sub></i>(4 STE12 binding sites)</td> |
− | <td> | + | <td>Transformational promoter</td> |
<td>223</td> | <td>223</td> | ||
</tr> | </tr> | ||
Line 226: | Line 226: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368027">BBa_2368027</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368027">BBa_2368027</a> | ||
</td> | </td> | ||
− | <td> | + | <td><i>P<sub>fus</sub></i>(6 STE12 binding sites)</td> |
− | <td> | + | <td>Transformational promoter</td> |
<td>249</td> | <td>249</td> | ||
</tr> | </tr> | ||
Line 234: | Line 234: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368006">BBa_2368006</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368006">BBa_2368006</a> | ||
</td> | </td> | ||
− | <td> | + | <td><i>P<sub>fus</sub></i>+<i>mRFP+CYC1T</i></td> |
<td>The reportor</td> | <td>The reportor</td> | ||
<td>1375</td> | <td>1375</td> | ||
Line 242: | Line 242: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368002">BBa_2368002</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368002">BBa_2368002</a> | ||
</td> | </td> | ||
− | <td> | + | <td><i>CYC1t</i> A terminator in</td> |
<td>the yeast</td> | <td>the yeast</td> | ||
<td>231</td> | <td>231</td> | ||
Line 250: | Line 250: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368029">BBa_2368029</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368029">BBa_2368029</a> | ||
</td> | </td> | ||
− | <td> | + | <td> <i>P<sub>fus</sub></i>(2 STE12 binding sites)</td> |
<td>Transformational promoter</td> | <td>Transformational promoter</td> | ||
<td>213</td> | <td>213</td> |
Revision as of 13:55, 27 October 2017
Biobrick | Name | Description | Length |
---|---|---|---|
Receptor expression | |||
BBa_2368003 | T1R2 | Human source Sweet-receptor | 2520 |
BBa_2368004 | T1R3 | Human source Sweet-receptor | 1989 |
BBa_2368007 | Gal 1/10-T1R2-CYC1 | Completed expression of the human source Sweet-receptor | 3449 |
BBa_2368008 | Gal 1/10-T1R3-ADH1 | Completed expression of the human source Sweet-receptor | 2814 |
BBa_2368023 | MYC+T1R2 overlap | Marker genes of the Sweet-receptor | 349 |
BBa_2368009 | HIS+T1R2 overlap | Marker genes of the Sweet-receptor | 50 |
BBa_2368024 | HIS+T1R3 overlap | Marker genes of the Sweet-receptor | 187 |
BBa_2368011 | FLAG+T1R3 overlap | Marker genes of the Sweet-receptor | 50 |
BBa_2368012 | YCP+T1R2 overlap | Marker genes of the Sweet-receptor | 740 |
BBa_2368013 | YCP+T1R3 overlap | Marker genes of the Sweet-receptor | 740 |
BBa_2368014 | BCP+T1R2 overlap | Marker genes of the Sweet-receptor | 710 |
BBa_2368015 | GFP+T1R2 overlap | Marker genes of the Sweet-receptor | 699 |
BBa_2368020 | BCP+T1R3 overlap | Marker genes of the Sweet-receptor | 710 |
Host engineeringn | |||
BBa_2368017 | 500bp Homologous arm+His | knocking out the sst2 gene | 2050 |
BBa_2368018 | 500bp Homologous arm+Ura | knocking out the far1 gene | 2050 |
BBa_2368016 | 500bp Homologous arm+Trp | knocking out the ste2 gene | 2071 |
BBa_2368021 | Modified Gα Schizosaccharomyces pombe | Signal transduction | 1421 |
BBa_2368022 | Modified Gα Caenorhabditis elegans | Signal transduction | 1418 |
BBa_2368028 | Gα | Signal transduction | 1421 |
Detect circuit | |||
BBa_2368005 | Pfus | Induced promoter | 438 |
BBa_2368026 | Pfus(4 STE12 binding sites) | Transformational promoter | 223 |
BBa_2368027 | Pfus(6 STE12 binding sites) | Transformational promoter | 249 |
BBa_2368006 | Pfus+mRFP+CYC1T | The reportor | 1375 |
BBa_2368002 | CYC1t A terminator in | the yeast | 231 |
BBa_2368029 | Pfus(2 STE12 binding sites) | Transformational promoter | 213 |