Difference between revisions of "Team:CCA San Diego/Improve"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Description">Description </a></li>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Design">Design</a></li>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Description#Results">Results </a></li>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Safety">Safety </a></li>
  
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<div class="column full_size">
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                            </ul>
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<h1>Improve</h1>
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                        </li>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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                        <li class="dropdown"><a href="/Team:CCA_San_Diego/InterLab" style="color:#217fba;">InterLab </a>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Model">Model</a></li>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Applied_Design">Applied Design</a></li>
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                                <li role="presentation"><a href="/Team:CCA_San_Diego/Engagement">Public Engagement</a></li>
  
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<center><h3>Improvement</h3></center>
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<center><h6>We have submitted this part as a plasmid backbone"</h6></center>
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<h6>RK2 Broad Host Range Vector with Kanamycin Resistance
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The goal of this improvement was to replace p15a origin of replication in pSB3K3 (http://parts.igem.org/Part:pSB3K3) with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).
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The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium</h6>  
  
  
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                        <h3>Social media</h3>
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                        <h3>Sponsors </h3>
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                            <li><a href="https://www.labfellows.com/">Lab Fellows</a></li>
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                            <li><a href="http://www.canyoncrestfoundation.org/">CCA Foundation</a></li>
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                            <li><a href="https://www.genscript.com/">GeneScript</a></li>
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                            <li><a href="https://www.idtdna.com/site">IDT</a></li>
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                            <li><a href="#">Quest Foundation</a></li>
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                            <li><a href="https://ccaigemsummercamp.weebly.com">CCA iGEM Summer Camps 2017</a></li>
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Revision as of 03:32, 28 October 2017


Improvement

We have submitted this part as a plasmid backbone"
RK2 Broad Host Range Vector with Kanamycin Resistance The goal of this improvement was to replace p15a origin of replication in pSB3K3 (http://parts.igem.org/Part:pSB3K3) with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms). The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium