Difference between revisions of "Team:CCA San Diego/Improve"

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Revision as of 03:51, 28 October 2017


Improvement

BBa_K2491030 short
We have submitted this part as a plasmid backbone

RK2 Broad Host Range Vector with Kanamycin Resistance


The goal of this improvement was to replace p15a origin of replication in pSB3K3 (Source) with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).

The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium.
Sequence and Features BBa_K2491030 SequenceAndFeatures Add more about the biology of this part here ===Usage and Biology=== Sequence and Features BBa_K2491030 SequenceAndFeatures Uncomment this to enable Functional Parameter display ===Functional Parameters=== BBa_K2491030 parameters