Difference between revisions of "Team:BIT-China/Notebook"
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<p class="my-content-li2">4.30-6.20 : heterologous expression of T1R2/T1R3 and characteristic. <a href="https://2017.igem.org/Team:BIT-China/Project/Sense">Click here to see more details.</a></p> | <p class="my-content-li2">4.30-6.20 : heterologous expression of T1R2/T1R3 and characteristic. <a href="https://2017.igem.org/Team:BIT-China/Project/Sense">Click here to see more details.</a></p> | ||
<h4 class="title-h4">4.30-5.15：modeling</h4> | <h4 class="title-h4">4.30-5.15：modeling</h4> | ||
| − | <p class="my-content-li2">4.30-5.5：finish several sweeteners’ as well as T1R2/T1R3 PDB file construction. Different sweeteners and numbers are docking to the sweet receptor T1R2/T1R3.</p> | + | <p class="my-content-li2">4.30-5.5：finish several sweeteners’ as well as T1R2/T1R3 PDB file construction. Different sweeteners and numbers are docking to the sweet receptor T1R2/T1R3.<a href="https://2017.igem.org/Team:BIT-China/Model/Docking">Click here to see more details.</a></p> |
| − | <p class="my-content-li2">5.5-5.15：MAPK pathway signal modeling</p> | + | <p class="my-content-li2">5.5-5.15：MAPK pathway signal modeling. <a href="https://2017.igem.org/Team:BIT-China/Model/GPCRpathway">Click here to see more details.</a></p> |
<h4 class="title-h4">5.2-8.20: pathway optimization</h4> | <h4 class="title-h4">5.2-8.20: pathway optimization</h4> | ||
<p class="my-content-li2">5.2-7.1 : single gene knock out</p> | <p class="my-content-li2">5.2-7.1 : single gene knock out</p> | ||
| − | <p class="my-content-li2">7.1-8.5 : knock out gene combination</p> | + | <p class="my-content-li2">7.1-8.5 : knock out gene combination.<a href="https://2017.igem.org/Team:BIT-China/Project/Transduction">Click here to see more details.</a></p> |
<p class="my-content-li2">8.5-8.20 : engineered strain characterization</p> | <p class="my-content-li2">8.5-8.20 : engineered strain characterization</p> | ||
<h4 class="title-h4">8.21-10.20: new pathway verification</h4> | <h4 class="title-h4">8.21-10.20: new pathway verification</h4> | ||
Revision as of 08:28, 28 October 2017
NOTEBOOK
Breakthrough
2.14-4.14: Recruiting team & Brainstorm
4.14-6.20: core part synthesis and heterologous expression
4.14-5.2 : core part-T1R2/T1R3 synthesis completely through OE-PCR.
4.30-6.20 : heterologous expression of T1R2/T1R3 and characteristic. Click here to see more details.
4.30-5.15：modeling
4.30-5.5：finish several sweeteners’ as well as T1R2/T1R3 PDB file construction. Different sweeteners and numbers are docking to the sweet receptor T1R2/T1R3.Click here to see more details.
5.5-5.15：MAPK pathway signal modeling. Click here to see more details.
5.2-8.20: pathway optimization
5.2-7.1 : single gene knock out
7.1-8.5 : knock out gene combination.Click here to see more details.
8.5-8.20 : engineered strain characterization
8.21-10.20: new pathway verification
8.21-9.13 : diction circuit establishment
9.13-9.26 : new pathway verification
9.27-10.10 : sweet meter verification
10.8-10.20 : parts standardization
For more details,please see our project.
