Difference between revisions of "Team:Sydney Australia/Parts"
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mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt"> | ||
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt"> | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt"> | ||
| − | <td width=" | + | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;mso-border-alt: |
| − | + | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt; | |
height:18.3pt"> | height:18.3pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size: | + | font-family:Quicksand"><o:p> </o:p></span></b></p> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | font-family:Quicksand">PART NAME <span class="GramE">/ <span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></b></p> |
| + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; | ||
| + | font-family:Quicksand"><o:p> </o:p></span></b></p> | ||
</td> | </td> | ||
| − | + | <td width="15%" style="width:15.3%;border:solid windowtext 1.0pt;border-left: | |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | <td width=" | + | |
| − | none;mso-border- | + | |
background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font- | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; |
| − | + | font-family:Quicksand">TYPE<o:p></o:p></span></b></p> | |
| − | + | ||
| − | + | ||
</td> | </td> | ||
| − | <td width="15%" style="width:15. | + | <td width="15%" style="width:15.6%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; | |
| − | + | font-family:Quicksand">SOURCE<o:p></o:p></span></b></p> | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-family:Quicksand"> | + | |
</td> | </td> | ||
| − | <td width=" | + | <td width="47%" style="width:47.7%;border:solid windowtext 1.0pt;border-left: |
| − | + | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
| − | mso-border- | + | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt"> |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:13.0pt; | |
| − | + | font-family:Quicksand">KEY FEATURES<o:p></o:p></span></b></p> | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font- | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:1;height:11.35pt"> |
<td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;border-top: | <td width="21%" style="width:21.4%;border:solid windowtext 1.0pt;border-top: | ||
none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p> |
</td> | </td> | ||
<td width="15%" style="width:15.3%;border-top:none;border-left:none; | <td width="15%" style="width:15.3%;border-top:none;border-left:none; | ||
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mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
<td width="15%" style="width:15.6%;border-top:none;border-left:none; | <td width="15%" style="width:15.6%;border-top:none;border-left:none; | ||
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mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N/A </span><span lang="EN-US" style="font-family: | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:13.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica; |
| − | + | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size: | |
| − | + | 13.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p> | |
| − | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | |
</td> | </td> | ||
<td width="47%" style="width:47.7%;border-top:none;border-left:none; | <td width="47%" style="width:47.7%;border-top:none;border-left:none; | ||
| Line 465: | Line 452: | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Novel single chain insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Novel single chain insulin |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p> | + | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> | + | (QRGGGSGGGQRR)<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> |
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p> | ||
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:2;height:114.0pt"> |
<td width="21%" rowspan="2" style="width:21.4%;border:solid windowtext 1.0pt; | <td width="21%" rowspan="2" style="width:21.4%;border:solid windowtext 1.0pt; | ||
border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-family:Quicksand">-Proinsulin<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000</a><o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000</a><o:p></o:p></span></p> |
</td> | </td> | ||
<td width="15%" rowspan="2" style="width:15.3%;border-top:none;border-left: | <td width="15%" rowspan="2" style="width:15.3%;border-top:none;border-left: | ||
| Line 485: | Line 474: | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p> |
</td> | </td> | ||
<td width="15%" style="width:15.6%;border-top:none;border-left:none; | <td width="15%" style="width:15.6%;border-top:none;border-left:none; | ||
| Line 491: | Line 480: | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span> Tag)<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span> |
| + | Tag)<o:p></o:p></span></p> | ||
</td> | </td> | ||
<td width="47%" rowspan="2" style="width:47.7%;border-top:none;border-left: | <td width="47%" rowspan="2" style="width:47.7%;border-top:none;border-left: | ||
| Line 497: | Line 487: | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Human proinsulin<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> |
| − | for targeting proteins to the periplasm<o:p></o:p></span></p> | + | tag for targeting proteins to the periplasm<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">N terminal 6x His Tag<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">N terminal 6x His Tag<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Extended ribosomal binding site<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Extended ribosomal binding |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand"><o:p> </o:p></span></p> | + | site<o:p></o:p></span></p> |
| + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand"><o:p> </o:p></span></p> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| − | <tr style="mso-yfti-irow: | + | <tr style="mso-yfti-irow:3;mso-yfti-lastrow:yes;height:114.0pt"> |
<td width="15%" style="width:15.6%;border-top:none;border-left:none; | <td width="15%" style="width:15.6%;border-top:none;border-left:none; | ||
border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt"> | ||
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-family:Quicksand">Human (Human proinsulin)<o:p></o:p></span></p> | + | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:13.0pt;font-family:Quicksand">Human (Human proinsulin)<o:p></o:p></span></p> |
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 13:48, 28 October 2017
GOLD
|
GOLD |
|||
|
PART
NAME / NO. |
TYPE |
SOURCE |
KEY
FEATURES |
|
YncM-Winsulin |
COMPOSITE PART |
Bacillus
subtilis (YncM secretion tag) |
Novel
short chain insulin analogue (Winsulin) with a 12AA
linker (QRGGGSGGGQRR) YncM secretion tag to translocate protein into
media N-terminal
6x His Tag purification 4x
GSS flexible linker TEV
protease cleavage site Extended
ribosome binding site |
|
Ecotin-Proinsulin |
COMPOSITE PART |
E. Coli (Ecotin
Tag) |
Human proinsulin N-terminal Ecotin
tag for targeting proteins to the periplasm N terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosomal binding
site |
|
Human (Human
proinsulin) |
|||
|
Cytoplasmic-Proinsulin BBa_K2417001 |
COMPOSITE PART |
Human (Human
proinsulin) |
Human
proinsulin N-terminal
6x His tag 4x
GGS flexible linker Trypsin
protease cleavage site for His-tag and C-peptide cleavage Extended ribosomal binding
site |
SILVER
|
PART NAME / NO. |
TYPE |
SOURCE |
KEY FEATURES |
|
Cytoplasmic-Winsulin |
COMPOSITE PART |
N/A – Winsulin is self designed from literature and mammalian insulins |
Novel single chain insulin
analogue (Winsulin) with a 12AA linker
(QRGGGSGGGQRR) N-terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site |
|
Ecotin-Proinsulin |
COMPOSITE PART |
E. Coli (Ecotin
Tag) |
Human proinsulin N-terminal Ecotin
tag for targeting proteins to the periplasm N terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosomal binding
site |
|
Human (Human proinsulin) |
BRONZE
|
BRONZE |
|||
|
PART NAME / NO. |
TYPE |
SOURCE |
KEY FEATURES |
|
Ecotin-Winsulin |
COMPOSITE PART |
E. Coli (Ecotin
Tag) |
Novel single chain insulin
analogue (Winsulin) with a 12AA linker
(QRGGGSGGGQRR) N-terminal Ecotin
tag for targeting proteins to the periplasm N-terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site |
|
N/A – Winsulin is self designed from literature and mammalian insulins |
|||
