Difference between revisions of "Team:GZHS-United/Notebook"

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<head>
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<title>Notebook</title>
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</head>
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<div class="header-banner" id="head">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Notebook">Notebook</a>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Timeline">Timeline</a>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/HP/Gold_Integrated">Gold & Integrated</a>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Engagement">Public Engagement</a>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Entrepreneurship">Entrepreneurship</a>
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</li>
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</nav>
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</ul>
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</div>
  
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<div class="column full_size">
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</div>
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</div>
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<h1>Notebook</h1>
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</div>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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</div>
 
</div>
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<div class="clear"></div>
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<div class="content" id="GZHS_content">
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<div class="container">  
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<div class="row" style="padding:60px 0;">
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<div class="column half_size">
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<table class="table">
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<h5>What should this page have?</h5>
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<thead>
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<ul>
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<tr>
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<li>Chronological notes of what your team is doing.</li>
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<th>Date</th>
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<li> Brief descriptions of daily important events.</li>
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<th>objection</th>
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<li>Pictures of your progress. </li>
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<th>result</th>
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<li>Mention who participated in what task.</li>
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<th>Analysis</th>
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</ul>
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<th>Solution</th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td>7-10</td>
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<td>1. Extract the genomic DNA of Bt and Bs.</td>
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<td>1. Only the genomic DNA of Bs were obtained. </td>
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<td>The Bt haven’t been actived.</td>
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<td>Culture Bt in LB to active it.</td>
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</tr>
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<tr>
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<td>7-11</td>
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<td>
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1. Extract the genomic DNA of Bt. <br>
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2. Acquire mtx gene form genomic DNA of Bs by PCR.
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</td>
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<td>
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1.  DNA electrophoresis showed that the strip appeared at 150bp.<br>
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2. A peal DNA strip in agarose gel electrophoresis were observed.
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</td>
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<td>1. The fragment we extracted were RNA. </td>
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<td>
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1. Use a more gentle method to broke the Gram-negative bacterium cell wall.<br>
 +
2. Optimize the PCR condition and try to use the diluted production as template.
 +
</td>
 +
</tr>
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<tr>
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<td>7-12</td>
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<td>
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1. Extract the genomic DNA of Bt. <br>
 +
2. Amplification of mtx1 gene.
 +
</td>
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<td>
 +
1. The genomic DNA of Bt were successfully obtained.<br>
 +
2. The mtx1 gene was successfully obtained.
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</td>
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<td>Boiling water-bath is a good method to broke the bacterium cell wall.</td>
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<td>/</td>
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</tr>
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</tbody>
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</table>
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</div>
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</div>
 
</div>
 
</div>
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</body>
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<div id="to_top">
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<img src="https://static.igem.org/mediawiki/2017/9/97/T--GZHS-United--toTop_buttom.png" onClick="toTop()"/>
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</div>
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<div id="next_page">
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<a href="https://2017.igem.org/Team:GZHS-United/Gallery"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--GZHS-United--right_buttom.png"/></a>
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</div>
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<script>
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dropdownOpen();
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});
  
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<div class="column half_size">
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var timer = null;
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<h5>Inspiration</h5>
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function toTop(){
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<p>You can see what others teams have done to organize their notes:</p>
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cancelAnimationFrame(timer);
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timer = requestAnimationFrame(function fn(){
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<ul>
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var oTop = document.body.scrollTop || document.documentElement.scrollTop;
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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if(oTop > 0){
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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document.body.scrollTop = document.documentElement.scrollTop = oTop - 50;
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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timer = requestAnimationFrame(fn);
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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}else{
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</ul>
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cancelAnimationFrame(timer);
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}
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</div>
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});
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}
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</html>

Revision as of 17:56, 28 October 2017

Notebook

Date objection result Analysis Solution
7-10 1. Extract the genomic DNA of Bt and Bs. 1. Only the genomic DNA of Bs were obtained. The Bt haven’t been actived. Culture Bt in LB to active it.
7-11 1. Extract the genomic DNA of Bt.
2. Acquire mtx gene form genomic DNA of Bs by PCR. 		1. DNA electrophoresis showed that the strip appeared at 150bp.
2. A peal DNA strip in agarose gel electrophoresis were observed. 		1. The fragment we extracted were RNA. 1. Use a more gentle method to broke the Gram-negative bacterium cell wall.
2. Optimize the PCR condition and try to use the diluted production as template.
7-12 1. Extract the genomic DNA of Bt.
2. Amplification of mtx1 gene. 		1. The genomic DNA of Bt were successfully obtained.
2. The mtx1 gene was successfully obtained. 		Boiling water-bath is a good method to broke the bacterium cell wall. /