Difference between revisions of "Team:GZHS-United/Notebook"

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1. Extract the genomic DNA of Bt. <br>
 
1. Extract the genomic DNA of Bt. <br>
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2. Amplification of mtx1 gene.  
 
2. Amplification of mtx1 gene.  
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<img src="https://static.igem.org/mediawiki/2017/3/35/T--GZHS-United--notebook2.png">
 
 
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1. The genomic DNA of Bt were successfully obtained.<br>
 
1. The genomic DNA of Bt were successfully obtained.<br>
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<img src="https://static.igem.org/mediawiki/2017/6/69/T--GZHS-United--notebook1.png"><br>
 
2. The mtx1 gene was successfully obtained.
 
2. The mtx1 gene was successfully obtained.
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<img src="https://static.igem.org/mediawiki/2017/3/35/T--GZHS-United--notebook2.png">
 
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<td>Boiling water-bath is a good method to broke the bacterium cell wall.</td>
 
<td>Boiling water-bath is a good method to broke the bacterium cell wall.</td>

Revision as of 18:05, 28 October 2017

Notebook

Date objection result Analysis Solution
7-10 1. Extract the genomic DNA of Bt and Bs. 1. Only the genomic DNA of Bs were obtained. The Bt haven’t been actived. Culture Bt in LB to active it.
7-11 1. Extract the genomic DNA of Bt.
2. Acquire mtx gene form genomic DNA of Bs by PCR. 		1. DNA electrophoresis showed that the strip appeared at 150bp.
2. A peal DNA strip in agarose gel electrophoresis were observed. 		1. The fragment we extracted were RNA. 1. Use a more gentle method to broke the Gram-negative bacterium cell wall.
2. Optimize the PCR condition and try to use the diluted production as template.
7-12 1. Extract the genomic DNA of Bt.
2. Amplification of mtx1 gene. 		1. The genomic DNA of Bt were successfully obtained.

2. The mtx1 gene was successfully obtained. 		Boiling water-bath is a good method to broke the bacterium cell wall. /
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