Difference between revisions of "Team:BostonU HW/Transformation"

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<title>Digestion</title>
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<title>Cell Lysis</title>
  
 
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#TITLE{
 
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
 
<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
 
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
 
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<img src="https://static.igem.org/mediawiki/2017/7/7a/MARS_Digestion.png" id="TITLE">
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<img src="https://static.igem.org/mediawiki/2017/3/33/MARS_Cell_Lysis.png" id="TITLE">
 
</div>
 
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<div class="main main-raised" style="margin-bottom:5%;">
 
<div class="main main-raised" style="margin-bottom:5%;">
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<h1>Summary</h1>
 
<h1>Summary</h1>
 
<div class="text" style="margin-bottom:3%;">
 
<div class="text" style="margin-bottom:3%;">
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DNA digestion, or DNA fragmentation, is a basic protocol in synthetic biology. This is typically performed prior to analysis of the DNA sequence, or in order to perform further protocols. Restriction enzymes are mixed and then incubated with DNA in a buffer solution. This yields DNA fragments cleaved at specific sites according to the enzymes used.
+
Cell lysis is a commonly used protocol in synthetic biology. It can be performed through a variety of different methods, however we had focused on chemical cellular lysis. Cell Lysis is used to extract and isolate DNA from a specific type of cell. This is an extremely important step in building genetic circuits in order to utilize specific coding regions in a cell's DNA. Chemical cell lysis involves introducing cells to a series of buffers in order to degrade the cell’s outer membrane and collect the DNA that is released.
 
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This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipett
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This microfluidic chip is designed to perform chemical cell lysis. Suspended cells and an lysing buffer would be mixed inside the cell. This mixture of buffer and cells would be then mixed with a neutralization buffer to prevent DNA degradation. The mixture would then move to the diamond chamber where the DNA binds magnetic particles. Lastly, an elution buffer would be input and to clean and release the DNA from the magnetic particles.
 
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<div class="tab-pane active" id="Design">
 
<div class="tab-pane active" id="Design">
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/2/28/MARS_Digestion_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/b/bb/MARS_Lysis_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center" style="margin-bottom:5%;">
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<div class="row text-center">
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<button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/f/fa/MARS_Digestion_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center" style="margin-bottom:5%;">
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<div class="row text-center">
 
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
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<div class="tab-pane" id="Mill">
 
<div class="tab-pane" id="Mill">
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/6/6b/MARS_Digestion_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/08/MARS_Lysis_M.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 
<div class="row">
 
<div class="row">
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<button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
 
</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-6 text-center">
 
<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/2/25/MARS_Digestion_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
 
<div class="row text-center">
 
<div class="row text-center">
−
<button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button>
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
 
</div>
 
</div>
 
</div>
 
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</div>
 
</div>
 
</div>
 
</div>
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<div class="container">
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<div class="col-md-6" style="margin-bottom:5%;">
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<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
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  <source src="https://static.igem.org/mediawiki/2017/4/47/MARS_LysisVid.mp4" type="video/mp4">
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</video>
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</div>
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<div class="col-md-6">
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<h1>Testing</h1>
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<div class="text">
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This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
 +
No biological material was inserted into this chip.
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</div>
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</div>
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</div>
 
</div>
 
</div>
 
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</div>

Revision as of 04:59, 29 October 2017

BostonU_HW

Cell Lysis

Summary

Cell lysis is a commonly used protocol in synthetic biology. It can be performed through a variety of different methods, however we had focused on chemical cellular lysis. Cell Lysis is used to extract and isolate DNA from a specific type of cell. This is an extremely important step in building genetic circuits in order to utilize specific coding regions in a cell's DNA. Chemical cell lysis involves introducing cells to a series of buffers in order to degrade the cell’s outer membrane and collect the DNA that is released.
This microfluidic chip is designed to perform chemical cell lysis. Suspended cells and an lysing buffer would be mixed inside the cell. This mixture of buffer and cells would be then mixed with a neutralization buffer to prevent DNA degradation. The mixture would then move to the diamond chamber where the DNA binds magnetic particles. Lastly, an elution buffer would be input and to clean and release the DNA from the magnetic particles.
Picture
Picture
Picture
Picture

Testing

This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip. No biological material was inserted into this chip.