Difference between revisions of "Team:BostonU HW/Ligation"
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND"> | <img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND"> | ||
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | <img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/1/15/MARS_Ligation.png" id="TITLE"> |
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<h1>Summary</h1> | <h1>Summary</h1> | ||
<div class="text" style="margin-bottom:3%;"> | <div class="text" style="margin-bottom:3%;"> | ||
| − | + | Ligation is a commonly used protocol in synthetic biology. In molecular cloning ligation is the process by which external DNA is inserted into a vector DNA, often a plasmid, using the enzyme DNA ligase. The newly formed DNA, or recombinant DNA, can then be analyzed or transformed. | |
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| − | This microfluidic chip | + | This microfluidic chip is designed to perform ligation. T4 DNA Ligase Buffer, vector DNA, insert DNA, water, and T4 DNA Ligase are metered on the chip and are then mixed together. The solution then undergoes incubation, after which the recombinant DNA solution can pipetted out from the chip and used in further molecular cloning procedures |
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<div class="tab-pane active" id="Design"> | <div class="tab-pane active" id="Design"> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
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<div class="row text-center" style="margin-bottom:5%;"> | <div class="row text-center" style="margin-bottom:5%;"> | ||
<button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button> | <button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button> | ||
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<div class="row text-center" style="margin-bottom:5%;"> | <div class="row text-center" style="margin-bottom:5%;"> | ||
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | <button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | ||
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| − | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/4/4c/MARS_Ligation_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row"> | <div class="row"> | ||
<button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button> | <button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button> | ||
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| − | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/c/c2/MARS_Ligation_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center"> | <div class="row text-center"> | ||
<button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button> | <button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button> | ||
Revision as of 05:54, 29 October 2017
Summary
Ligation is a commonly used protocol in synthetic biology. In molecular cloning ligation is the process by which external DNA is inserted into a vector DNA, often a plasmid, using the enzyme DNA ligase. The newly formed DNA, or recombinant DNA, can then be analyzed or transformed.
This microfluidic chip is designed to perform ligation. T4 DNA Ligase Buffer, vector DNA, insert DNA, water, and T4 DNA Ligase are metered on the chip and are then mixed together. The solution then undergoes incubation, after which the recombinant DNA solution can pipetted out from the chip and used in further molecular cloning procedures
