Difference between revisions of "Team:TU Darmstadt/Demonstrate"
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<p>iGEM is coping with the problem of different groups measuring fluorescence differently, in different units and evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP. | <p>iGEM is coping with the problem of different groups measuring fluorescence differently, in different units and evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP. | ||
The study should be carried out and examined in different laboratories all over the world. | The study should be carried out and examined in different laboratories all over the world. | ||
Revision as of 16:31, 29 October 2017
ChiTUcare
InterLab Measurement Studies
iGEM is coping with the problem of different groups measuring fluorescence differently, in different units and evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP.
The study should be carried out and examined in different laboratories all over the world.
Our team followed the standardized procedure guiding us through transformation, inoculation and measurement process.
For more information: Fourth International InterLab Measurement Study
Following below you can find our results.
Calibration
OD600 reference point
Several measurements of LUDOX-S40 and H²O were spectrometric analyzed below.
| LUDOX-S40 | H²O | |
| Replicate 1 | 0,041700002 | 0,035300002 |
| Replicate 2 | 0,042399999 | 0,035 |
| Replicate 3 | 0,043000001 | 0,035700001 |
| Replicate 4 | 0,043000001 | 0,034699999 |
| Arithmetic Mean | 0,042525001 | 0,035175 |
| Corrected Absorbance 600 | 0,007350001 | |
| Reference OD600 | 0,0425 | |
| Correction Factor | 5,78231249 |
Fluorescein standard curve:
The fluorescence of a dilution series, fluorescein in four replicates were measured in standard modes in our Tecan plate reader (infinite 200Pro).
Generated standard curve of fluorescence for different fluorescein concentrations can be found below.
Cell Measurement
Fluorescence Measurement
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Absorbance 600 Measurement
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Fluorescein / OD600
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Abschlusssatz!!!
