Difference between revisions of "Team:TU Darmstadt/Demonstrate"

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<h3>Chitin Synthase NodC</h3>
 
<h3>Chitin Synthase NodC</h3>
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<p><img src="https://static.igem.org/mediawiki/2017/1/13/T--TU_Darmstadt--Synthase_Pikto_fertig-neu.png" style="float:left;margin-right:1%;width:15%">
 
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The functionality of the NodC enzyme was verified by performing the <i>UDP-Glo<sup>TM</sup> Glycosyltransferase</i> Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.  
 
The functionality of the NodC enzyme was verified by performing the <i>UDP-Glo<sup>TM</sup> Glycosyltransferase</i> Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.  
 
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<center><img src="https://static.igem.org/mediawiki/2017/6/69/T--TU_Darmstadt--40ng_mit_allem.png" width="80%"></center>
 
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Revision as of 21:01, 29 October 2017

MainPage

Proof of Concept

Here we want to give you a short tour about what we achieved in the project. We show our results and give a view on the resulting product, a enzyme sensing hydrogel.

Chitin Synthase NodC

The functionality of the NodC enzyme was verified by performing the UDP-GloTM Glycosyltransferase Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.