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</div> | </div> | ||
</section> | </section> | ||
− | <section id=" | + | |
− | <h3>Chitin | + | |
+ | <section id="CDA"><div class="container"> | ||
+ | <h3>Chitin Deacetylase NodB</h3> | ||
<div class="post-it" style="margin-bottom:30px; display: flex; align-items:center;"> | <div class="post-it" style="margin-bottom:30px; display: flex; align-items:center;"> | ||
− | <p><img src="https://static.igem.org/mediawiki/2017/ | + | <p><img src="https://static.igem.org/mediawiki/2017/c/c7/T--TU_Darmstadt--Deacetylase_Pikto.png" style="float:left;margin-right:1%;width:15%"> |
− | + | Under Construction | |
</p></div> | </p></div> | ||
<center> | <center> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/2/22/T--TU_Darmstadt--NodBresults2.gif" width="80%"> |
− | <figcaption style="width:80%;"> < | + | <figcaption style="width:80%;"> |
+ | </figcaption></center> | ||
+ | </div> | ||
+ | </section> | ||
+ | |||
+ | |||
+ | <section id="Hydrogel"><div class="container"> | ||
+ | <h3>Hydrogel</h3> | ||
+ | <div class="post-it" style="margin-bottom:30px; display: flex; align-items:center;"> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2017/e/ed/T--TU_Darmstadt--Hydrogel_Piktogramm.png" style="float:left;margin-right:1%;width:15%"> | ||
+ | We manufactured several hydrogels containing non-toxic and cost-effectively gelling agent to form optimal wound dressings. Our hydrogels could be formed with basic laboratory equipment at any shape and could easily be adjust to the affected tissues. | ||
+ | </p></div> | ||
+ | <center> | ||
+ | <img src="" width="80%"> | ||
+ | <figcaption style="width:80%;"> | ||
</figcaption></center> | </figcaption></center> | ||
</div> | </div> |
Revision as of 21:35, 29 October 2017
![](https://static.igem.org/mediawiki/2017/3/3d/LogoOWL.png)
ChiTUcare
Proof of Concept
Here we want to give you a short tour about what we achieved in the project. We show our results and give a view on the resulting product, a enzyme sensing hydrogel.
Chitin Synthase NodC
The functionality of the NodC enzyme was verified by performing the UDP-Glo™ Glycosyltransferase Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.
See more Details
![](https://static.igem.org/mediawiki/2017/6/69/T--TU_Darmstadt--40ng_mit_allem.png)
Chitin Deacetylase NodB
Under Construction
![](https://static.igem.org/mediawiki/2017/2/22/T--TU_Darmstadt--NodBresults2.gif)
Hydrogel
We manufactured several hydrogels containing non-toxic and cost-effectively gelling agent to form optimal wound dressings. Our hydrogels could be formed with basic laboratory equipment at any shape and could easily be adjust to the affected tissues.