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Revision as of 21:40, 29 October 2017
![](https://static.igem.org/mediawiki/2017/3/3d/LogoOWL.png)
ChiTUcare
Proof of Concept
Here we want to give you a short tour about what we achieved in the project. We show our results and give a view on the resulting product, a enzyme sensing hydrogel.
Chitin Synthase NodC
The functionality of the NodC enzyme was verified by performing the UDP-Glo™ Glycosyltransferase Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.
See more Details
![](https://static.igem.org/mediawiki/2017/6/69/T--TU_Darmstadt--40ng_mit_allem.png)
Chitin Deacetylase NodB
Under Construction
![](https://static.igem.org/mediawiki/2017/2/22/T--TU_Darmstadt--NodBresults2.gif)
Hydrogel
We manufactured several hydrogels containing non-toxic and cost-effectively gelling agent to form optimal wound dressings. Our hydrogels could be formed with basic laboratory equipment at any shape and could easily be adjust to the affected tissues.
![](https://static.igem.org/mediawiki/2017/4/4c/T--TU_Darmstadt--lieblingsbild_hydrog.png)