Difference between revisions of "Team:NYMU-Taipei/Contribution"
| Line 65: | Line 65: | ||
.igem_2017_content_wrapper a { | .igem_2017_content_wrapper a { | ||
| − | color:# | + | color:#38af2f; |
| − | text-decoration-color:# | + | text-decoration-color:#38af2f; |
} | } | ||
.igem_2017_content_wrapper a:hover { | .igem_2017_content_wrapper a:hover { | ||
| − | color:# | + | color:#f99595; |
} | } | ||
| Line 177: | Line 177: | ||
<font class='h7'>pPIGBACK</font> | <font class='h7'>pPIGBACK</font> | ||
</a> | </a> | ||
| − | <p style='padding-top:0px;'>On the basis of previous research, we designed and constructed a useful vector pPIGBACK for microalgae transformation. pPIGBACK is a powerful construct with NSII, AmpR, double terminator, ORI, and PrbcL, and it is able to embark on gene double-crossover homologous recombination in <i>Synechococcus elongatus</i> PCC 7942 genome. <a href='https://2017.igem.org/Team:NYMU-Taipei/Pigments'>(see more detail: | + | <p style='padding-top:0px;'>On the basis of previous research, we designed and constructed a useful vector pPIGBACK for microalgae transformation. pPIGBACK is a powerful construct with NSII, AmpR, double terminator, ORI, and PrbcL, and it is able to embark on gene double-crossover homologous recombination in <i>Synechococcus elongatus</i> PCC 7942 genome. <a href='https://2017.igem.org/Team:NYMU-Taipei/Pigments'>(see more detail: Pigments - Backbone Design)</a> |
</p> | </p> | ||
</div> | </div> | ||
| Line 203: | Line 203: | ||
<div class='right_column'> | <div class='right_column'> | ||
<h2>Constructs & Transformants</h2> | <h2>Constructs & Transformants</h2> | ||
| − | <p>We sent | + | <p>We sent 14 parts to iGEM and successfully transformed pigments into <i>Synechococcus elongates</i> PCC 7942.</p> |
<div id='s4' class='expandable' style='height:32px;'> | <div id='s4' class='expandable' style='height:32px;'> | ||
| Line 209: | Line 209: | ||
<font class='h7'>Yellow Microalgae with CrtZ </font> | <font class='h7'>Yellow Microalgae with CrtZ </font> | ||
</a> | </a> | ||
| − | <p style='padding-top:0px;'>We have successfully used our microalgae transformation platform to transform CrtZ into <i>Synechococcus elongates</i> PCC 7942 and the transformant are more yellow than wild type! <a href='https://2017.igem.org/Team:NYMU-Taipei/Pigments'>(see more detail: | + | <p style='padding-top:0px;'>We have successfully used our microalgae transformation platform to transform CrtZ into <i>Synechococcus elongates</i> PCC 7942 and the transformant are more yellow than wild type! <a href='https://2017.igem.org/Team:NYMU-Taipei/Pigments'>(see more detail: Pigments - Functional Test)</a> |
</p> | </p> | ||
</div> | </div> | ||
| Line 218: | Line 218: | ||
<font class='h7'>Endolysin-Holin-NrtA</font> | <font class='h7'>Endolysin-Holin-NrtA</font> | ||
</a> | </a> | ||
| − | <p style='padding-top:0px;'>We successfully transformed NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803 to <i>E.coli</i>. The engineering <i>E.coli</i> which secretes NrtA protein can catch nitrate (NO<sub>3</sub><sup>-</sup>) and nitrite (NO<sub>2</sub><sup>-</sup>) to induce algae undergoing nitrogen starvation. Due to biosafety concern, we also improved a suicide mechanism endolysin-holin and successfully constructed it. Moreover, we even grouped endolysin-holin and NrtA into a powerful part. <a href='https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation'>(see more detail: | + | <p style='padding-top:0px;'>We successfully transformed NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803 to <i>E.coli</i>. The engineering <i>E.coli</i> which secretes NrtA protein can catch nitrate (NO<sub>3</sub><sup>-</sup>) and nitrite (NO<sub>2</sub><sup>-</sup>) to induce algae undergoing nitrogen starvation. Due to biosafety concern, we also improved a suicide mechanism endolysin-holin and successfully constructed it. Moreover, we even grouped endolysin-holin and NrtA into a powerful part. <a href='https://2017.igem.org/Team:NYMU-Taipei/Nitrogen_starvation'>(see more detail: Nitrogen Starvation - Functional Test)</a> |
</p> | </p> | ||
</div> | </div> | ||
| Line 235: | Line 235: | ||
<div class='right_column'> | <div class='right_column'> | ||
<h2>Modeling</h2> | <h2>Modeling</h2> | ||
| − | <p>We established 12 models to check and predict the results of the experiments. These models are extremely important to our project! <a href='https://2017.igem.org/Team:NYMU-Taipei/Model'>(see more detail: | + | <p>We established 12 models to check and predict the results of the experiments. These models are extremely important to our project! <a href='https://2017.igem.org/Team:NYMU-Taipei/Model'>(see more detail: Modeling)</a> |
</p> | </p> | ||
</div> | </div> | ||
| Line 250: | Line 250: | ||
<div class='right_column'> | <div class='right_column'> | ||
<h2>Unique and Detailed Protocols for the Public</h2> | <h2>Unique and Detailed Protocols for the Public</h2> | ||
| − | <p>We provided unique and detailed protocols of our experiments to the public. Students, teachers, scientists all over the world and future iGEM teams can repeat our experiments or design their own experiments with these protocols. <a href='https://2017.igem.org/Team:NYMU-Taipei/Notebook'>(see more detail: | + | <p>We provided unique and detailed protocols of our experiments to the public. Students, teachers, scientists all over the world and future iGEM teams can repeat our experiments or design their own experiments with these protocols. <a href='https://2017.igem.org/Team:NYMU-Taipei/Notebook'>(see more detail: Notebook - Protocols)</a> |
</p> | </p> | ||
</div> | </div> | ||
Revision as of 08:43, 30 October 2017
Contribution
Microalgae Transformation Platform
To transform pigments genes into Synechococcus elongatus PCC 7942, we created a well-designed platform of microalgae transformation.
Constructs & Transformants
We sent 14 parts to iGEM and successfully transformed pigments into Synechococcus elongates PCC 7942.
Modeling
We established 12 models to check and predict the results of the experiments. These models are extremely important to our project! (see more detail: Modeling)
Unique and Detailed Protocols for the Public
We provided unique and detailed protocols of our experiments to the public. Students, teachers, scientists all over the world and future iGEM teams can repeat our experiments or design their own experiments with these protocols. (see more detail: Notebook - Protocols)
Sharing Genetic Engineering Technology
Chung Cheng University – Fusion PCR
Taipei America School – Site Directed Mutagenesis
Assistance to Establish New iGEM Teams
We help students in Chung Hsing University and Taipei Wego Senior High School to establish their own iGEM teams. We are looking forward to seeing them at Boston in 2018!
Experimental Technology
In addition to our comprehensive microalgae transformation platform, to achieve our goals, we also applied lots of experimental technology. Although most of our team members are freshman in college, we tried hard to learn relatively complicated experimental methods. Moreover, we realized that, with more experimental technology and knowledge, we can be closer to our goals and do more things for the world. Therefore, we strongly encourage future iGEM team to explore new technology. Deeper thinking and thrive on challenge that come along with the journey of learning are as important as the technology itself.
Fusion PCR
We used fusion PCR in NrtA construct, pPIGBACK construct and fusion of pigments and the promoter prbcL.
Sticky End PCR
We used sticky end PCR in holin, endolysin, NrtA and CrtZ construct.
3 Piece Overlap PCR
We used 3 piece overlap PCR in lycopene and pPIGBACK construct.
Site Directed Mutagenesis
We used site directed mutagenesis in pPIGBACK and PrbcL construct.
Electroporation
We used electroporation in IndC construct.
Making Competent Cell
We made competent cell in electroporation experiments.
French Pressure Cell Press NrtA
We use French pressure cell press in NrtA functional test.
