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| | {{:Team:Sydney_Australia/Templates/NavBar}} | | {{:Team:Sydney_Australia/Templates/NavBar}} |
| − |
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| − | <html>
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| − | <head>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
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| − |
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| − | <style>
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| − |
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| − | /* our special font */
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| − | @font-face {
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| − | font-family: 'banaue';
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| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
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| − | font-weight: normal;
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| − | font-style: large;
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| − | }
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| − |
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| − | #please h1 {
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| − | font-family: "banaue", sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 60px;
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| − | }
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| − |
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| − | #please h4 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 16px;
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| − | LINE-HEIGHT:20px;
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| − |
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| − | }
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| − |
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| − | #please p {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − |
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| − | }
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| − |
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| − | #please h3 {
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| − | font-family: 'quicksand', sans-serif;
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| − | color: #db4d4f;
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| − |
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| − | }
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| − |
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| − | #please h2 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #a2cca3;
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| − | font-size: 20.8px;
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| − |
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| − | }
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| − |
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| − | #please h5 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 22px;
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| − | font-weight: bold;
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| − |
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| − | }
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| − |
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| − | #please h6 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 20px;
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| − |
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| − | }
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| − |
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| − | .crispy {
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| − | image-rendering: -moz-crisp-edges;
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| − | image-rendering: -o-crisp-edges;
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| − | image-rendering: -webkit-optimize-contrast;
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| − | -ms-interpolation-mode: nearest-neighbor;
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| − | padding:0px;
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| − | width:90%;
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| − | margin-top: 0px;
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| − | margin-left:5%;,
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| − | margin-right:5%;
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| − | background: #fff;
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| − | }
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| − |
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| − | .img-center {
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| − | image-rendering: -moz-crisp-edges;
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| − | image-rendering: -o-crisp-edges;
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| − | image-rendering: -webkit-optimize-contrast;
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| − | -ms-interpolation-mode: nearest-neighbor;
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| − | width:80%;
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| − | }
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| − |
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| − | .insert {
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| − | height: 68px;
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| − | width: 100%;
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| − | background-color:#fff;
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| − | }
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| − | .insert2 {
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| − | height: 150px;
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| − | width: 100%
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| − | background-color:#fff;
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| − | }
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| − |
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| − | .box {
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| − | background:#fff;
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| − | border:0px solid;
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| − | border-color: #e2e2e2;
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| − | margin-top: 0px;
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| − | box-sizing: border-box;
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| − | background-clip: padding-box;
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| − | padding:0 20px 20px 20px;
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| − | min-height:500px;
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| − | min-width:400px
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| − | }
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| − |
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| − | .bannerbox {
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| − | background:#e2e2e2;
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| − | border:0px solid;
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| − | border-color: #e2e2e2;
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| − | margin-top: 0px;
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| − | box-sizing: border-box;
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| − | background-clip: padding-box;
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| − | padding:0 20px 20px 20px;
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| − | height:750px;
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| − | width:100%;
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| − | }
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| − |
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| − | .content {
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| − | padding:0px;
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| − | width:80%;
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| − | margin-top: 0px;
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| − | margin-left:10%;
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| − | margin-right:10%;
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| − | }
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| − |
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| − |
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| − | tr, tf {color:#3e3f3f;}
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| − | tr td:hover { background: #e2e2e2; color: #3e3f3f; }
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| − | p, caption, h1 {font-family:'Quicksand';color:#3e3f3f;}
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| − |
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| − | select {
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| − | width:30%;
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| − | height:10vh;
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| − | background-color:#e2e2e2;
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| − | position:relative;
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| − | }
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| − |
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| − | .box1, .box2, .box3, .box4, .box5 {
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| − | background:#f6d562;
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| − | border:0px solid;
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| − | border-color: #f6d562;
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| − | margin-top: 10px;
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| − | box-sizing: border-box;
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| − | background-clip: padding-box;
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| − | padding:0 10px 10px 10px;
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| − | max-height:2px;
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| − | min-width:800px;
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| − | }
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| − |
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| − | .box2 {
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| − | background:#cfcfcf;
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| − | border-color: #cfcfcf;
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| − | }
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| − |
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| − | .box3 {
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| − | background:#cd8b65;
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| − | border-color: #cd8b65;
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| − | }
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| − |
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| − | .box4 {
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| − | background:#fad990;
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| − | border-color: #fad990;
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| − | }
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| − |
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| − | .box5 {
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| − | background:#93bcc1;
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| − | border-color: #93bcc1;
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| − | }
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| − |
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| − | .center {
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| − | position: absolute;
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| − | right:4px;
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| − | top:80px;
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| − | }
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| − |
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| − | </style>
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| − | </head>
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| − | <body>
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| − |
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| − |
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| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
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| − | <div class = "center">
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| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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| − | </div>
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| − | </div>
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| − |
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| − | <div class ="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
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| − | <center><h1>GOLD</h1></center>
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| − | <div class="box1"></div>
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| − | <div class="row" style="height:2vw;"></div>
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| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
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| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
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| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | <tr style="mso-yfti-irow:1;height:78.9pt">
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| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
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| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| | | | |
| | <html> | | <html> |
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| | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; |
| | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt"> | | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt"> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
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| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
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| − | translocate protein into media<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
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| − | purification<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
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| − | demonstrated that it works:<o:p></o:p></span></p>
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| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
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| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
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| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
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| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
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| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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| − | <!--[endif]--><o:p></o:p></span></p>
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| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
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| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
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| − | font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
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| − | </tr>
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| − | <tr style="mso-yfti-irow:2;height:206.35pt">
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| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
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| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <div align="center">
| |
| − |
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
| |
| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − |
| |
| − | </div>
| |
| − | </div>
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| − |
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="row" style="height:10vw;">
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| − |
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| − | </div>
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| − | </body>
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| − | </html>
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| − | {{:Team:Sydney_Australia/templates/Footer}}
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| − |
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| − |
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| − | <html>
| |
| − | <head>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
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| − |
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| − | <style>
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| − |
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| − | /* our special font */
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| − | <div class ="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
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| − | <center><h1>GOLD</h1></center>
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| − | <div class="box1"></div>
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| − | <div class="row" style="height:2vw;"></div>
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| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
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| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
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| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
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| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
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| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
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| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
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| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
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| − | </tbody></table>
| |
| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
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| − | <div class="row" style="height:2vw;"></div>
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| − | <div align="center">
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| − | <div align="center">
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| − |
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| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
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| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
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| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
| |
| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
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| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
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| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | </tr>
| |
| − | </tbody></table>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="row" style="height:10vw;">
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| − |
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| − | </div>
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| − | </body>
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| − | </html>
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| − | {{:Team:Sydney_Australia/templates/Footer}}
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| − |
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| − |
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| − | <html>
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| − | <head>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
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| − |
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| − | <style>
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| − |
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| − | /* our special font */
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| − | @font-face {
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| − | font-family: 'banaue';
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| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
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| − | font-weight: normal;
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| − | font-style: large;
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| − | }
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| − |
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| − | #please h1 {
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| − | font-family: "banaue", sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 60px;
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| − | }
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| − |
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| − | #please h4 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 16px;
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| − | LINE-HEIGHT:20px;
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| − |
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| − | }
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| − |
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| − | #please p {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − |
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| − | }
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| − |
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| − | #please h3 {
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| − | font-family: 'quicksand', sans-serif;
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| − | color: #db4d4f;
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| − |
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| − | }
| |
| − |
| |
| − | #please h2 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #a2cca3;
| |
| − | font-size: 20.8px;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please h5 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 22px;
| |
| − | font-weight: bold;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please h6 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 20px;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | .crispy {
| |
| − | image-rendering: -moz-crisp-edges;
| |
| − | image-rendering: -o-crisp-edges;
| |
| − | image-rendering: -webkit-optimize-contrast;
| |
| − | -ms-interpolation-mode: nearest-neighbor;
| |
| − | padding:0px;
| |
| − | width:90%;
| |
| − | margin-top: 0px;
| |
| − | margin-left:5%;,
| |
| − | margin-right:5%;
| |
| − | background: #fff;
| |
| − | }
| |
| − |
| |
| − | .img-center {
| |
| − | image-rendering: -moz-crisp-edges;
| |
| − | image-rendering: -o-crisp-edges;
| |
| − | image-rendering: -webkit-optimize-contrast;
| |
| − | -ms-interpolation-mode: nearest-neighbor;
| |
| − | width:80%;
| |
| − | }
| |
| − |
| |
| − | .insert {
| |
| − | height: 68px;
| |
| − | width: 100%;
| |
| − | background-color:#fff;
| |
| − | }
| |
| − | .insert2 {
| |
| − | height: 150px;
| |
| − | width: 100%
| |
| − | background-color:#fff;
| |
| − | }
| |
| − |
| |
| − | .box {
| |
| − | background:#fff;
| |
| − | border:0px solid;
| |
| − | border-color: #e2e2e2;
| |
| − | margin-top: 0px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 20px 20px 20px;
| |
| − | min-height:500px;
| |
| − | min-width:400px
| |
| − | }
| |
| − |
| |
| − | .bannerbox {
| |
| − | background:#e2e2e2;
| |
| − | border:0px solid;
| |
| − | border-color: #e2e2e2;
| |
| − | margin-top: 0px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 20px 20px 20px;
| |
| − | height:750px;
| |
| − | width:100%;
| |
| − | }
| |
| − |
| |
| − | .content {
| |
| − | padding:0px;
| |
| − | width:80%;
| |
| − | margin-top: 0px;
| |
| − | margin-left:10%;
| |
| − | margin-right:10%;
| |
| − | }
| |
| − |
| |
| − |
| |
| − | tr, tf {color:#3e3f3f;}
| |
| − | tr td:hover { background: #e2e2e2; color: #3e3f3f; }
| |
| − | p, caption, h1 {font-family:'Quicksand';color:#3e3f3f;}
| |
| − |
| |
| − | select {
| |
| − | width:30%;
| |
| − | height:10vh;
| |
| − | background-color:#e2e2e2;
| |
| − | position:relative;
| |
| − | }
| |
| − |
| |
| − | .box1, .box2, .box3, .box4, .box5 {
| |
| − | background:#f6d562;
| |
| − | border:0px solid;
| |
| − | border-color: #f6d562;
| |
| − | margin-top: 10px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 10px 10px 10px;
| |
| − | max-height:2px;
| |
| − | min-width:800px;
| |
| − | }
| |
| − |
| |
| − | .box2 {
| |
| − | background:#cfcfcf;
| |
| − | border-color: #cfcfcf;
| |
| − | }
| |
| − |
| |
| − | .box3 {
| |
| − | background:#cd8b65;
| |
| − | border-color: #cd8b65;
| |
| − | }
| |
| − |
| |
| − | .box4 {
| |
| − | background:#fad990;
| |
| − | border-color: #fad990;
| |
| − | }
| |
| − |
| |
| − | .box5 {
| |
| − | background:#93bcc1;
| |
| − | border-color: #93bcc1;
| |
| − | }
| |
| − |
| |
| − | .center {
| |
| − | position: absolute;
| |
| − | right:4px;
| |
| − | top:80px;
| |
| − | }
| |
| − |
| |
| − | </style>
| |
| − | </head>
| |
| − | <body>
| |
| − |
| |
| − |
| |
| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
| |
| − | <div class = "center">
| |
| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class ="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | {{:Team:Sydney_Australia/Templates/NavBar}}
| |
| − |
| |
| − | <html>
| |
| − | <head>
| |
| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
| |
| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
| |
| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
| |
| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
| |
| − |
| |
| − | <style>
| |
| − |
| |
| − | /* our special font */
| |
| − | @font-face {
| |
| − | font-family: 'banaue';
| |
| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
| |
| − | font-weight: normal;
| |
| − | font-style: large;
| |
| − | }
| |
| − |
| |
| − | #please h1 {
| |
| − | font-family: "banaue", sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 60px;
| |
| − | }
| |
| − |
| |
| − | #please h4 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 16px;
| |
| − | LINE-HEIGHT:20px;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please p {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please h3 {
| |
| − | font-family: 'quicksand', sans-serif;
| |
| − | color: #db4d4f;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please h2 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #a2cca3;
| |
| − | font-size: 20.8px;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please h5 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 22px;
| |
| − | font-weight: bold;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | #please h6 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 20px;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | .crispy {
| |
| − | image-rendering: -moz-crisp-edges;
| |
| − | image-rendering: -o-crisp-edges;
| |
| − | image-rendering: -webkit-optimize-contrast;
| |
| − | -ms-interpolation-mode: nearest-neighbor;
| |
| − | padding:0px;
| |
| − | width:90%;
| |
| − | margin-top: 0px;
| |
| − | margin-left:5%;,
| |
| − | margin-right:5%;
| |
| − | background: #fff;
| |
| − | }
| |
| − |
| |
| − | .img-center {
| |
| − | image-rendering: -moz-crisp-edges;
| |
| − | image-rendering: -o-crisp-edges;
| |
| − | image-rendering: -webkit-optimize-contrast;
| |
| − | -ms-interpolation-mode: nearest-neighbor;
| |
| − | width:80%;
| |
| − | }
| |
| − |
| |
| − | .insert {
| |
| − | height: 68px;
| |
| − | width: 100%;
| |
| − | background-color:#fff;
| |
| − | }
| |
| − | .insert2 {
| |
| − | height: 150px;
| |
| − | width: 100%
| |
| − | background-color:#fff;
| |
| − | }
| |
| − |
| |
| − | .box {
| |
| − | background:#fff;
| |
| − | border:0px solid;
| |
| − | border-color: #e2e2e2;
| |
| − | margin-top: 0px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 20px 20px 20px;
| |
| − | min-height:500px;
| |
| − | min-width:400px
| |
| − | }
| |
| − |
| |
| − | .bannerbox {
| |
| − | background:#e2e2e2;
| |
| − | border:0px solid;
| |
| − | border-color: #e2e2e2;
| |
| − | margin-top: 0px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 20px 20px 20px;
| |
| − | height:750px;
| |
| − | width:100%;
| |
| − | }
| |
| − |
| |
| − | .content {
| |
| − | padding:0px;
| |
| − | width:80%;
| |
| − | margin-top: 0px;
| |
| − | margin-left:10%;
| |
| − | margin-right:10%;
| |
| − | }
| |
| − |
| |
| − |
| |
| − | tr, tf {color:#3e3f3f;}
| |
| − | tr td:hover { background: #e2e2e2; color: #3e3f3f; }
| |
| − | p, caption, h1 {font-family:'Quicksand';color:#3e3f3f;}
| |
| − |
| |
| − | select {
| |
| − | width:30%;
| |
| − | height:10vh;
| |
| − | background-color:#e2e2e2;
| |
| − | position:relative;
| |
| − | }
| |
| − |
| |
| − | .box1, .box2, .box3, .box4, .box5 {
| |
| − | background:#f6d562;
| |
| − | border:0px solid;
| |
| − | border-color: #f6d562;
| |
| − | margin-top: 10px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 10px 10px 10px;
| |
| − | max-height:2px;
| |
| − | min-width:800px;
| |
| − | }
| |
| − |
| |
| − | .box2 {
| |
| − | background:#cfcfcf;
| |
| − | border-color: #cfcfcf;
| |
| − | }
| |
| − |
| |
| − | .box3 {
| |
| − | background:#cd8b65;
| |
| − | border-color: #cd8b65;
| |
| − | }
| |
| − |
| |
| − | .box4 {
| |
| − | background:#fad990;
| |
| − | border-color: #fad990;
| |
| − | }
| |
| − |
| |
| − | .box5 {
| |
| − | background:#93bcc1;
| |
| − | border-color: #93bcc1;
| |
| − | }
| |
| − |
| |
| − | .center {
| |
| − | position: absolute;
| |
| − | right:4px;
| |
| − | top:80px;
| |
| − | }
| |
| − |
| |
| − | </style>
| |
| − | </head>
| |
| − | <body>
| |
| − |
| |
| − |
| |
| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
| |
| − | <div class = "center">
| |
| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class ="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <div align="center">
| |
| − |
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
| |
| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
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| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
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| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
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| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | </tbody></table>
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| − |
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="row" style="height:10vw;">
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| − |
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| − | </div>
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| − | </body>
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| − | </html>
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| − | {{:Team:Sydney_Australia/templates/Footer}}
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| − |
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| − | <html>
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| − | <head>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
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| − |
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| − | <style>
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| − |
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| − | /* our special font */
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| − | @font-face {
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| − | font-weight: normal;
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| − | font-style: large;
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| − | }
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| − | #please h1 {
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| − | font-family: "banaue", sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 60px;
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| − | }
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| − | #please h4 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 16px;
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| − | LINE-HEIGHT:20px;
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| − | }
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| − | #please p {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | }
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| − | #please h3 {
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| − | font-family: 'quicksand', sans-serif;
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| − | color: #db4d4f;
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| − |
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| − | }
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| − |
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| − | #please h2 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #a2cca3;
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| − | font-size: 20.8px;
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| − |
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| − | }
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| − |
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| − | #please h5 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 22px;
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| − | font-weight: bold;
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| − |
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| − | }
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| − |
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| − | #please h6 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 20px;
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| − |
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| − | }
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| − |
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| − | .crispy {
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| − | image-rendering: -moz-crisp-edges;
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| − | image-rendering: -o-crisp-edges;
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| − | image-rendering: -webkit-optimize-contrast;
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| − | -ms-interpolation-mode: nearest-neighbor;
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| − | padding:0px;
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| − | width:90%;
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| − | margin-top: 0px;
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| − | margin-left:5%;,
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| − | margin-right:5%;
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| − | background: #fff;
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| − | }
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| − |
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| − | .img-center {
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| − | image-rendering: -moz-crisp-edges;
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| − | image-rendering: -o-crisp-edges;
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| − | image-rendering: -webkit-optimize-contrast;
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| − | -ms-interpolation-mode: nearest-neighbor;
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| − | width:80%;
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| − | }
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| − | .insert {
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| − | height: 68px;
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| − | width: 100%;
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| − | background-color:#fff;
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| − | }
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| − | .insert2 {
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| − | height: 150px;
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| − | width: 100%
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| − | background-color:#fff;
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| − | }
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| − | .box {
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| − | background:#fff;
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| − | border:0px solid;
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| − | border-color: #e2e2e2;
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| − | margin-top: 0px;
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| − | box-sizing: border-box;
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| − | background-clip: padding-box;
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| − | padding:0 20px 20px 20px;
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| − | min-height:500px;
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| − | min-width:400px
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| − | .bannerbox {
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| − | background:#e2e2e2;
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| − | border:0px solid;
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| − | margin-top: 0px;
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| − | box-sizing: border-box;
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| − | background-clip: padding-box;
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| − | padding:0 20px 20px 20px;
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| − | height:750px;
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| − | width:100%;
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| − | }
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| − | .content {
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| − | padding:0px;
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| − | width:80%;
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| − | margin-top: 0px;
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| − | margin-left:10%;
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| − | margin-right:10%;
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| − | }
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| − |
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| − |
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| − | tr, tf {color:#3e3f3f;}
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| − | tr td:hover { background: #e2e2e2; color: #3e3f3f; }
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| − | p, caption, h1 {font-family:'Quicksand';color:#3e3f3f;}
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| − | select {
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| − | width:30%;
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| − | height:10vh;
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| − | background-color:#e2e2e2;
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| − | position:relative;
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| − | }
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| − |
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| − | .box1, .box2, .box3, .box4, .box5 {
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| − | background:#f6d562;
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| − | border:0px solid;
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| − | border-color: #f6d562;
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| − | margin-top: 10px;
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| − | box-sizing: border-box;
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| − | background-clip: padding-box;
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| − | padding:0 10px 10px 10px;
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| − | max-height:2px;
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| − | min-width:800px;
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| − | .box2 {
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| − | border-color: #cfcfcf;
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| − | .box3 {
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| − | border-color: #cd8b65;
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| − | border-color: #fad990;
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| − | .box5 {
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| − | background:#93bcc1;
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| − | border-color: #93bcc1;
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| − | }
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| − |
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| − | .center {
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| − | position: absolute;
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| − | right:4px;
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| − | top:80px;
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| − | }
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| − |
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| − | </style>
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| − | </head>
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| − | <body>
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| − |
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| − |
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| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
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| − | <div class = "center">
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| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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| − | </div>
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| − | </div>
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| − |
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| − | <div class ="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
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| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <div align="center">
| |
| − |
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
| |
| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − |
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | <div class="row" style="height:10vw;">
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| − |
| |
| − | </div>
| |
| − | </body>
| |
| − | </html>
| |
| − | {{:Team:Sydney_Australia/templates/Footer}}
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| − |
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| − |
| |
| − | <html>
| |
| − | <head>
| |
| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
| |
| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
| |
| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
| |
| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
| |
| − |
| |
| − | <style>
| |
| − |
| |
| − | /* our special font */
| |
| − | @font-face {
| |
| − | font-family: 'banaue';
| |
| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
| |
| − | font-weight: normal;
| |
| − | font-style: large;
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| − | }
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| − |
| |
| − | #please h1 {
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| − | font-family: "banaue", sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 60px;
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| − | }
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| − |
| |
| − | #please h4 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 16px;
| |
| − | LINE-HEIGHT:20px;
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| − |
| |
| − | }
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| − |
| |
| − | #please p {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
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| − |
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| − | }
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| − |
| |
| − | #please h3 {
| |
| − | font-family: 'quicksand', sans-serif;
| |
| − | color: #db4d4f;
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| − |
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| − | }
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| − |
| |
| − | #please h2 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #a2cca3;
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| − | font-size: 20.8px;
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| − |
| |
| − | }
| |
| − |
| |
| − | #please h5 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 22px;
| |
| − | font-weight: bold;
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| − |
| |
| − | }
| |
| − |
| |
| − | #please h6 {
| |
| − | font-family: 'Quicksand', sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 20px;
| |
| − |
| |
| − | }
| |
| − |
| |
| − | .crispy {
| |
| − | image-rendering: -moz-crisp-edges;
| |
| − | image-rendering: -o-crisp-edges;
| |
| − | image-rendering: -webkit-optimize-contrast;
| |
| − | -ms-interpolation-mode: nearest-neighbor;
| |
| − | padding:0px;
| |
| − | width:90%;
| |
| − | margin-top: 0px;
| |
| − | margin-left:5%;,
| |
| − | margin-right:5%;
| |
| − | background: #fff;
| |
| − | }
| |
| − |
| |
| − | .img-center {
| |
| − | image-rendering: -moz-crisp-edges;
| |
| − | image-rendering: -o-crisp-edges;
| |
| − | image-rendering: -webkit-optimize-contrast;
| |
| − | -ms-interpolation-mode: nearest-neighbor;
| |
| − | width:80%;
| |
| − | }
| |
| − |
| |
| − | .insert {
| |
| − | height: 68px;
| |
| − | width: 100%;
| |
| − | background-color:#fff;
| |
| − | }
| |
| − | .insert2 {
| |
| − | height: 150px;
| |
| − | width: 100%
| |
| − | background-color:#fff;
| |
| − | }
| |
| − |
| |
| − | .box {
| |
| − | background:#fff;
| |
| − | border:0px solid;
| |
| − | border-color: #e2e2e2;
| |
| − | margin-top: 0px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 20px 20px 20px;
| |
| − | min-height:500px;
| |
| − | min-width:400px
| |
| − | }
| |
| − |
| |
| − | .bannerbox {
| |
| − | background:#e2e2e2;
| |
| − | border:0px solid;
| |
| − | border-color: #e2e2e2;
| |
| − | margin-top: 0px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 20px 20px 20px;
| |
| − | height:750px;
| |
| − | width:100%;
| |
| − | }
| |
| − |
| |
| − | .content {
| |
| − | padding:0px;
| |
| − | width:80%;
| |
| − | margin-top: 0px;
| |
| − | margin-left:10%;
| |
| − | margin-right:10%;
| |
| − | }
| |
| − |
| |
| − |
| |
| − | tr, tf {color:#3e3f3f;}
| |
| − | tr td:hover { background: #e2e2e2; color: #3e3f3f; }
| |
| − | p, caption, h1 {font-family:'Quicksand';color:#3e3f3f;}
| |
| − |
| |
| − | select {
| |
| − | width:30%;
| |
| − | height:10vh;
| |
| − | background-color:#e2e2e2;
| |
| − | position:relative;
| |
| − | }
| |
| − |
| |
| − | .box1, .box2, .box3, .box4, .box5 {
| |
| − | background:#f6d562;
| |
| − | border:0px solid;
| |
| − | border-color: #f6d562;
| |
| − | margin-top: 10px;
| |
| − | box-sizing: border-box;
| |
| − | background-clip: padding-box;
| |
| − | padding:0 10px 10px 10px;
| |
| − | max-height:2px;
| |
| − | min-width:800px;
| |
| − | }
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| − |
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| − | .box2 {
| |
| − | background:#cfcfcf;
| |
| − | border-color: #cfcfcf;
| |
| − | }
| |
| − |
| |
| − | .box3 {
| |
| − | background:#cd8b65;
| |
| − | border-color: #cd8b65;
| |
| − | }
| |
| − |
| |
| − | .box4 {
| |
| − | background:#fad990;
| |
| − | border-color: #fad990;
| |
| − | }
| |
| − |
| |
| − | .box5 {
| |
| − | background:#93bcc1;
| |
| − | border-color: #93bcc1;
| |
| − | }
| |
| − |
| |
| − | .center {
| |
| − | position: absolute;
| |
| − | right:4px;
| |
| − | top:80px;
| |
| − | }
| |
| − |
| |
| − | </style>
| |
| − | </head>
| |
| − | <body>
| |
| − |
| |
| − |
| |
| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
| |
| − | <div class = "center">
| |
| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class ="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | {{:Team:Sydney_Australia/Templates/NavBar}}
| |
| − |
| |
| − | <html>
| |
| − | <head>
| |
| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
| |
| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
| |
| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
| |
| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
| |
| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
| |
| − |
| |
| − | <style>
| |
| − |
| |
| − | /* our special font */
| |
| − | @font-face {
| |
| − | font-family: 'banaue';
| |
| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
| |
| − | font-weight: normal;
| |
| − | font-style: large;
| |
| − | }
| |
| − |
| |
| − | #please h1 {
| |
| − | font-family: "banaue", sans-serif;
| |
| − | color: #3e3f3f;
| |
| − | font-size: 60px;
| |
| − | }
| |
| − |
| |
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| − | <body>
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| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
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| − | <div class = "center">
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| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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| − | </div>
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| − | </div>
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| − |
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| − | <div class ="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
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| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
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| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
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| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | </tr>
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| − | </tbody></table>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
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| − | <div class="row" style="height:2vw;"></div>
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| − | <div align="center">
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| − | <div align="center">
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| − |
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| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
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| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
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| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
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| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
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| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
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| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
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| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
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| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
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| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
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| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | </tbody></table>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − | </div>
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| − | </body>
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| − | </html>
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| − | {{:Team:Sydney_Australia/templates/Footer}}
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| − |
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| − | <html>
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| − | <head>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
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| − |
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| − | <style>
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| − |
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| − | /* our special font */
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| − | @font-face {
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| − | font-family: 'banaue';
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| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
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| − | font-weight: normal;
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| − | font-style: large;
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| − | }
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| − | #please h1 {
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| − | font-family: "banaue", sans-serif;
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| − | color: #3e3f3f;
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| − | font-size: 60px;
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| − | LINE-HEIGHT:20px;
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| − | #please h3 {
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| − | font-family: 'quicksand', sans-serif;
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| − | #please h2 {
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| − | font-family: 'Quicksand', sans-serif;
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| − | font-family: 'Quicksand', sans-serif;
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| − | .box1, .box2, .box3, .box4, .box5 {
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| − | position: absolute;
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| − | top:80px;
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| − | </style>
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| − | </head>
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| − | <body>
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| − |
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| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
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| − | <div class = "center">
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| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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| − | </div>
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| − | </div>
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| − |
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| − | <div class ="row" style="height:5vw;"></div>
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| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:19.05pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | </tbody></table>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
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| − | <center><h1>BRONZE</h1></center>
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| − | <div class="box3"></div>
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| − | <div class="row" style="height:2vw;"></div>
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| − | <div align="center">
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| − | <div align="center">
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| − |
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| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
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| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
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| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | <tr style="mso-yfti-irow:1;height:35.15pt">
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| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
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| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
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| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
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| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | </tbody></table>
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| − | </div>
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| − | </div>
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| − | </body>
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| − | </html>
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| − | {{:Team:Sydney_Australia/templates/Footer}}
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| − |
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| − | {{:Team:Sydney_Australia/Templates/NavBar}}
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| − | <html>
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| − | <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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| − | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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| − | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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| − | <style>
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| − |
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| − | /* our special font */
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| − | @font-face {
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| − | font-family: 'banaue';
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| − | src: url('https://static.igem.org/mediawiki/2017/b/b0/T--Sydney_Australia--Wiki_Font_Banaue-Regular.Media-File.ogg');
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| − | .crispy {
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| − | </style>
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| − | </head>
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| − | <body>
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| − | <div class = "bannerbox" style ="background-color:#e2e2e2">
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| − | <div class = "center">
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| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Sydney_Australia--PartsBanner.gif" width="70%">
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| − | </div>
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| − | </div>
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| − |
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| − | <div class ="row" style="height:5vw;"></div>
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| − |
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| − | <div class = "row">
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| − | <div class = "content" id="please">
| |
| − | <center><h1>GOLD</h1></center>
| |
| − | <div class="box1"></div>
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| − | <div class="row" style="height:2vw;"></div>
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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| − | height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
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| − | <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>WHAT IS IT GOLD?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:78.9pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <div align="center">
| |
| − |
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
| |
| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:10vw;">
| |
| − |
| |
| − | </div>
| |
| − | </body>
| |
| − | </html>
| |
| − | {{:Team:Sydney_Australia/templates/Footer}}
| |
| − |
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <div align="center">
| |
| − |
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;height:35.15pt">
| |
| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | Tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:10vw;">
| |
| − |
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| − | </div>
| |
| − | </body>
| |
| − | </html>
| |
| − | {{:Team:Sydney_Australia/templates/Footer}}
| |
| − |
| |
| − |
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
| |
| − | (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
| |
| − | translocate protein into media<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
| |
| − | purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
| |
| − | ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
| |
| − | the media in which the cells were growing did. <span class="GramE">Therefore</span>
| |
| − | the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;height:206.35pt">
| |
| − | <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
| |
| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
| |
| − | tag)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
| |
| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
| |
| − | border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
| |
| − | <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
| |
| − | <!--[endif]--><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
| |
| − | transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
| |
| − | for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
| |
| − | insulin-sensitive cell lines and saw an increase in glycogen synthesis and
| |
| − | glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:3;height:72.85pt">
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
| |
| − | <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes"> </span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.88%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
| |
| − | proinsulin)<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="29%" style="width:29.62%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
| |
| − | for His-tag and C-peptide cleavage<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
| |
| − | site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
| |
| − | improved previous parts:<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">completing their
| |
| − | sequence<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
| |
| − | ribosomal binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
| |
| − | N-terminal His tag for purification<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
| |
| − | font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
| |
| − | <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
| |
| − | cell lines and saw an increase in glycogen synthesis and glucose oxidation
| |
| − | above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>SILVER</h1></center>
| |
| − | <div class="box2"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/<span style="mso-spacerun:yes"> </span>NO.</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
| |
| − | none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.56%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.12%;border-top:none;border-left:none;
| |
| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
| |
| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| |
| − | Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
| |
| − | parts by showing that they work in producing insulin. We performed an ELISA
| |
| − | assay testing for properly folded insulin, and detected the presence of
| |
| − | Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
| |
| − | results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row" style="height:5vw;"></div>
| |
| − |
| |
| − | <div class = "row">
| |
| − | <div class = "content" id="please">
| |
| − | <center><h1>BRONZE</h1></center>
| |
| − | <div class="box3"></div>
| |
| − | <div class="row" style="height:2vw;"></div>
| |
| − | <div align="center">
| |
| − | <div align="center">
| |
| − |
| |
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
| |
| − | mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
| |
| − | <tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
| |
| − | <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
| |
| − | solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
| |
| − | height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
| |
| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
| |
| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
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| − | </td>
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| − | <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
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| − | none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
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| − | </td>
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| − | </tr>
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| − | <tr style="mso-yfti-irow:1;height:35.15pt">
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| − | <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
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| − | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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| − | padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
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| − | </td>
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| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
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| − | Tag)<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | </td>
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| − | <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
| |
| − | analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
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| − | tag for targeting proteins to the periplasm<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
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| − | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
| |
| − | the registry, for other teams to use in the future<o:p></o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
| |
| − | <td width="12%" style="width:12.48%;border-top:none;border-left:none;
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| − | border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
| |
| − | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
| |
| − | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
| |
| − | mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
| |
| − | 14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
| |
| − | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p> </o:p></span></p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − |
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="row" style="height:10vw;">
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| − | </div>
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| − | </body>
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| − | </html>
| |
| − | {{:Team:Sydney_Australia/templates/Footer}}
| |
| − |
| |
| | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p> | | <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p> |
| | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p> | | <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p> |
