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| − | <h3>Esterases and Lipases</h3>
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| − | <!--<https://static.igem.org/mediawiki/2017/4/45/EsteraseResults.png">-->
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| − | <img src="https://static.igem.org/mediawiki/2017/7/7f/EsteraseundLipase.png" class="img-responsive"/>
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| − | </div>
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| − | <h4>Enzyme activity assay: Esterases</h4>
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| − | <div class="col-xs-12 col-sm-10 col-md-10">
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| − | <p>In the following figures enzyme activities of the supernatant of the esterases EstCS2 and LIpB are shown. All results were obtained from biological triplicates. The detailed method for the activity determination is shown here: LINK
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| − | At the N-terminal end of the esterase EstCS2 a signal peptide (PelB) is added. Therefore the gene induction leads to enzyme expression and the expression of the signalpeptide and the secretion of the enzyme is enabled.
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| − | </p>
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| − | <!--<https://static.igem.org/mediawiki/2017/4/45/EsteraseResults.png">-->
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| − | <img src="https://static.igem.org/mediawiki/2017/4/45/EsteraseResults.png" class="img-responsive"/>
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| − | <h8>Figure 3: Illustration of the esterase activity [U] of EstCS2 in the supernatant dependend on time and substrate concentration (2.5, 5, 10, 15, 20 mM). The standard abbreviation was calculated from biological triplicates.</h8>
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| − | </div>
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| − | <p>The enzyme activity of EstCS2 raises with the induction level for gene expression and with the substrate concentration. A maximum of 246,5 U is gained with 20 mM p-Nitrophenyl butyrate and 1 mM arabinose. The enzyme activities between the induction levels of 2 mM arabinose and 3 mM arabinose isn’t different. The enzyme activity is highest with an induction level of 1 mM arabinose instead of an induction level of 2 or 3 mM arabinose. This could be explained with less movement flexibility of the esterases if high gene expression rates are induced. We would further recommend to induce the gene expression with less arabinose concentrations and investigate the enzyme activity with 0.1 mM, 0.5 mM and 1 mM arabinose. </p>
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| − | <!--<https://static.igem.org/mediawiki/2017/a/a0/EsteraseResults2.png">-->
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| − | <img src="https://static.igem.org/mediawiki/2017/a/a0/EsteraseResults2.png" class="img-responsive"/>
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| − | <h8>Figure 4: Illustration of the esterase activity [U] of E. coli wilde types in the supernatant dependend on time and substrate concentration (2.5, 5, 10, 15, 20 mM). The standard abbreviation was calculated from biological triplicates.
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| − | </h8>
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| − | <p>In comparison we investigated the enzyme activity of two E. coli wilde type strains (E. coli DH5α and MG1655). We aimed to aquire knowledge about the esterase activity of the wild typs to identify the effect of genetically engineered organisms. The figures show less enzyme activity of the wild types in comparison to the genetically engineered E. coli that contain the plasmid with the esterase genes. This means that the investigated EstCS2 is an appropriate enzyme to degradade fat layers on hair.
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| − | <h3>Rose and Limonene Fragrance</h3>
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| − | <!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">-->
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| − | <img src="https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png" class="img-responsive"/>
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| − | </div>
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| − | <h4>Rose Fragrance</h4>
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| − | <div class="col-xs-12 col-sm-10 col-md-10">
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| − | <p>HIER TEXT REINKOPIEREN
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| − | <h8>Figure 9: </h8>
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| − | <p>HIER TEXT REINKOPIEREN</p>
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| − | <h8>Figure 10: HIER TEXT REINKOPIEREN
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| − | <p>HIER TEXT REINKOPIEREN</P>
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| − | <h8>Figure 11:HIER TEXT REINKOPIEREN </h8>
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| − | <p>HIER TEXT REINKOPIEREN</p>
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