PART NO.

PART NAME

SOURCE

DESCRIPTION

BBa_K2417004

Glycine Glycine Serine (x4) Linker Motif A

Designed

·      Linker motif used to provide spacer region

·      Used to expose affinity purification tags such as 6x His tag

·      Serine and glycine used as they are small and flexible

BBa_K2417006

Human Proinsulin Coding Sequence

Humans

·      Coding sequence for human proinsulin, sourced from NCIB

·      Consists of A, B and C chains, but C-peptide is cleaved out

·      Contains 3 disulphide bonds

·      Contains arginine residues adjacent to C-peptide to allow cleavage

BBa_K2417007

Winsulin – Single Chain Insulin Analogue

Designed

·      Consists of short linker peptide instead of C-peptide

·      Does not require cleavage therefore simplifies production procedures

·      Linker peptide raises pI of insulin analogue (long lasting after administration)

·      Increased thermostability (based on other single chain insulin analogues tested)

BBa_K2417008

6x Poly-Histidine Tag codon optimized for use in E. coli

Designed (optimized for use in Escherichia coli)

·      Codon optimized for use in E. coli

·      Used to immobilize ions on a chromatography affinity column (nickel, cobalt or copper)

·      Small, and easy to add to the ends of proteins of interest

BBa_K2417009

Extended ribosome binding site

T7 major capsid protein

·      High ribosomal recruitment efficiency

·      Sourced from the pET15b vectors

BBa_K2417010

Ecotin Periplasmic Signal Peptide

Escherichia coli

·      Small periplasmic, homodimeric protein

·      Upon fusion to N-terminus of protein will translocate protein to periplasm of E. coli

·      Periplasm is an oxidative environment ideal for protein folding, and simplifies extraction due to eliminating the need for purifying inclusion bodies from the cytoplasm

·      Periplasm has reduced proteases

BBa_K2417011

YncM Bacillus Secretion Peptide

Bacillus subtilis

·      Can be fused to the N-terminus of proteins to induce secretion to the surrounding media when proteins are expressed in Bacillus subtilis

·      Eliminates many steps in the purification process

BBa_K2417012

TEV Protease Cleavage Sequence

N/A

·      Sequence for which TEV protease is specific to

·      Can be added between protein of interest and peptides that require removal, such as purification tags

BBa_K2417013

Glycine Glycine Serine (x4) Linker

Motif B

Designed

·      Linker motif used to provide spacer region

·      Used to expose affinity purification tags such as 6x His tag

·      Serine and glycine used as they are small and flexible