Difference between revisions of "Team:REC-CHENNAI/Notebook"

Revision as of 15:29, 30 October 2017

February
15	Finalized team
15	Created a group on WhatsApp. After a lot of reading, we got an idea, something about Lactoferrin.
15-20	Reading reading Zzzz
20	Team met to understand the registration process
21	Had a discussion and concluded that Lf is too big a protein to work with in this short time span. Dropped it.
22	Back to square one. No project idea. Split ourselves into 3 groups, each focusing on a different problem statement.
April
10	Brainstorming and Fundraising
May
02	Break for end semester exams
03	Whole team met Ramit. He agreed to mentor our team.
10	Opened our iGEM kit
10-21	Lab construction and renovation in progress.Lab construction and renovation in progress. Meanwhile literature work , brainstorming for project ideas.
15-20	Decided to work with AMPs Came across papers related to Latarcin Decided to work with this particular peptide Lab construction and renovation in progress.
June
01-09	Brainstorming for applications. Lab construction and renovation in progress.
10	First skype session with Carolina. Finalized our project idea
11	Team was split into wet lab, interlab study and HP team
13	First team fight :P
13-25	Placed order for all chemicals and kits required
26	Met Dr.Ng.Iboyaima Singh and Dr.G.R.Gopi to discuss our Delivery system.
27	Team meeting to discuss about mutant latarcin. Something seemed off. We realized that people were still not comfortable working with each other. Decided to talk it out like adults :P
28	Team bonding time. Played basketball. Went out for lunch.
29-30	Preparing buffers.
July
02	Competent cell preparation
03	Transformation of plasmid from the iGEM kit
05	No growth, Failed.
06-17	Alter protocol- prepare competent cells- transform- fail terribly- repeat.
15-16	Two members of our team attended the All India iGEM Meet at IISER, Pune.
20	Got our first transformation results.
26	Conducted a workshop for school students.
27-30	Designed and ordered our gblocks.
August
01-12	Plasmid isolation and restriction digestion optimisation. Worked on improving our transformation efficiency.
13	Conducted Biofair with SVCE. Turned out pretty well despite the fluctuating weather.
14-30	Placed order for our primers. Started working on the wiki. Worked on our questionnaire for the HP
September
02	Ligation refused to work out. Decided to move on to interlab study
04-20	Interlab study
21-24	Back to working on ligation in the lab.
25-26	US VISA interview.VISA approved!
27	Skype session with UCopenhagen about Podcasts.
28-30	Compilation of HP work.
October
02	Gandhi Jayanthi Celebrations.
03-10	Restriction-Ligation-Transformation - SUCCESS!Restriction-Ligation-Transformation - SUCCESS!
11-12	Worked on Podcasts.
13-20	Realized that Jamboree Preparation was way overdue! Started working on our Poster and Presentation.
21-30	Presentation practice. Final touches on Wiki. Get ready for the Jamboree.
November
01-07	Study-Crib-Write semester exam-Cry.Repeat.

February

Finalized team

Created a group on WhatsApp. After a lot of reading, we got an idea, something about Lactoferrin.

15-20

Reading reading Zzzz

Team met to understand the registration process

Had a discussion and concluded that Lf is too big a protein to work with in this short time span. Dropped it.

Back to square one. No project idea. Split ourselves into 3 groups, each focusing on a different problem statement.

April

Brainstorming and Fundraising

May

Break for end semester exams

Whole team met Ramit. He agreed to mentor our team.

Opened our iGEM kit

10-21

Lab construction and renovation in progress.Lab construction and renovation in progress.
Meanwhile literature work , brainstorming for project ideas.

15-20

Decided to work with AMPs
Came across papers related to Latarcin
Decided to work with this particular peptide
Lab construction and renovation in progress.

June

01-09

Brainstorming for applications.
Lab construction and renovation in progress.

First skype session with Carolina.
Finalized our project idea

Team was split into wet lab, interlab study and HP team

First team fight :P

13-25

Placed order for all chemicals and kits required

Met Dr.Ng.Iboyaima Singh and Dr.G.R.Gopi to discuss our Delivery system.

Team meeting to discuss about mutant latarcin.
Something seemed off. We realized that people were still not comfortable working with each other. Decided to talk it out like adults :P

Team bonding time. Played basketball. Went out for lunch.

29-30

Preparing buffers.

July

Competent cell preparation

Transformation of plasmid from the iGEM kit

No growth, Failed.

06-17

Alter protocol- prepare competent cells- transform- fail terribly- repeat.

15-16

Two members of our team attended the All India iGEM Meet at IISER, Pune.

Got our first transformation results.

Conducted a workshop for school students.

27-30

Designed and ordered our gblocks.

August

01-12

Plasmid isolation and restriction digestion optimisation.
Worked on improving our transformation efficiency.

Conducted Biofair with SVCE. Turned out pretty well despite the fluctuating weather.

14-30

Placed order for our primers.
Started working on the wiki.
Worked on our questionnaire for the HP

September

Ligation refused to work out.
Decided to move on to interlab study

04-20

Interlab study

21-24

Back to working on ligation in the lab.

25-26

US VISA interview.VISA approved!

Skype session with UCopenhagen about Podcasts.

28-30

Compilation of HP work.

October

Gandhi Jayanthi Celebrations.

03-10

Restriction-Ligation-Transformation - SUCCESS!Restriction-Ligation-Transformation - SUCCESS!

11-12

Worked on Podcasts.

13-20

Realized that Jamboree Preparation was way overdue!
Started working on our Poster and Presentation.

21-30

Presentation practice.
Final touches on Wiki.
Get ready for the Jamboree.

November

01-07

Study-Crib-Write semester exam-Cry.Repeat.

@@ Line 8: / Line 8: @@
 		<div class="wrapper">
-				<p style="text-indent:50px">This is our first time in iGEM and the past couple of months has been nothing short of hectic.
+				<p style="text-indent:100px">This is our first time in iGEM and the past couple of months has been nothing short of hectic. </p>
-<br>
+<p style="text-indent:100px">
 Scroll down to read our timeline - a journey of nights that turned into days with lab partners that turned into family.</p>