Difference between revisions of "Team:Stuttgart/Rose and Limonene Fragrance"

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<h8>Figure 11: Agaraose-gel electrophoresis of purified rose-PCR products</h8>
 
<h8>Figure 11: Agaraose-gel electrophoresis of purified rose-PCR products</h8>
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  <p>The overlap PCR products were assembled by Gibson Assembly (see protocols) in a pSB1K3 backbone.
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  The assembled roseplasmids were sucessfully transformed into DH5alpha <i>E.coli</i> cells.
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<h8>Figure 9: Sequenced rose plasmid (pET28a-ATF1, 7601 bp) from Guo et al. </h8>
 
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  <p>HIER TEXT REINKOPIEREN</p>
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Revision as of 20:06, 30 October 2017

Results

Rose and Limonene Fragrance

Rose Fragrance

The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10). The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR. The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.

Figure 9: Sequenced rose plasmid (pET28a-ATF1, 7601 bp) from Guo et al.
Figure 10: Sequenced rose plasmid (pET28a-KDC-YjgB-ARO8, 11.106 bp) from Guo et al.
Figure 11: Agaraose-gel electrophoresis of purified rose-PCR products

The overlap PCR products were assembled by Gibson Assembly (see protocols) in a pSB1K3 backbone. The assembled roseplasmids were sucessfully transformed into DH5alpha E.coli cells.

Figure 9: Sequenced rose plasmid (pET28a-ATF1, 7601 bp) from Guo et al.
Figure 10: HIER TEXT REINKOPIEREN

HIER TEXT REINKOPIEREN

Figure 11:HIER TEXT REINKOPIEREN

HIER TEXT REINKOPIEREN