Difference between revisions of "Team:Stuttgart/Rose and Limonene Fragrance"
| Line 27: | Line 27: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <br> | ||
<div class="row section"> | <div class="row section"> | ||
<div class="col-xs-6 col-sm-5 col-md-5"> | <div class="col-xs-6 col-sm-5 col-md-5"> | ||
| Line 33: | Line 34: | ||
<h8>Figure 9: Sequenced rose plasmid (pET28a-ATF1, 7601 bp) from Guo et al. </h8> | <h8>Figure 9: Sequenced rose plasmid (pET28a-ATF1, 7601 bp) from Guo et al. </h8> | ||
</div> | </div> | ||
| − | <div class="col-xs-6 col-sm- | + | <div class="col-xs-6 col-sm-4 col-md-4"> |
<!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">--> | <!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">--> | ||
<img src="https://static.igem.org/mediawiki/2017/7/77/PETKDCYjgBAro8.png" class="img-responsive"/> | <img src="https://static.igem.org/mediawiki/2017/7/77/PETKDCYjgBAro8.png" class="img-responsive"/> | ||
<h8>Figure 10: Sequenced rose plasmid (pET28a-KDC-YjgB-ARO8, 11.106 bp) from Guo et al. </h8> | <h8>Figure 10: Sequenced rose plasmid (pET28a-KDC-YjgB-ARO8, 11.106 bp) from Guo et al. </h8> | ||
</div> | </div> | ||
| − | <div class="col-xs-4 col-sm- | + | <div class="col-xs-4 col-sm-3 col-md-3"> |
<!--<https://static.igem.org/mediawiki/2017/d/d1/Gelrose1.png">--> | <!--<https://static.igem.org/mediawiki/2017/d/d1/Gelrose1.png">--> | ||
<img src="https://static.igem.org/mediawiki/2017/f/fa/Rosegel.png" class="img-responsive"/> | <img src="https://static.igem.org/mediawiki/2017/f/fa/Rosegel.png" class="img-responsive"/> | ||
| Line 45: | Line 46: | ||
</div> | </div> | ||
<div class="row section"> | <div class="row section"> | ||
| − | <div class="col-xs-12 col-sm- | + | <div class="col-xs-12 col-sm-5 col-md-5"> |
<p>The overlap PCR products were assembled by Gibson Assembly (see protocols) in a pSB1K3 backbone. | <p>The overlap PCR products were assembled by Gibson Assembly (see protocols) in a pSB1K3 backbone. | ||
| − | The assembled | + | The assembled rose plasmids were sucessfully transformed into DH5alpha <i>E.coli</i> cells. |
</p> | </p> | ||
</div> | </div> | ||
| − | <div class="col-xs-6 col-sm- | + | <div class="col-xs-6 col-sm-7 col-md-7"> |
<!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">--> | <!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">--> | ||
<img src="https://static.igem.org/mediawiki/2017/a/a3/PlatterosepETKDCYjgBAro8.png" class="img-responsive"/> | <img src="https://static.igem.org/mediawiki/2017/a/a3/PlatterosepETKDCYjgBAro8.png" class="img-responsive"/> | ||
| − | <h8>Figure 9: | + | <h8>Figure 9: Sucessful transformation of assembled rose plasmid in DH5alpha <i>E.coli</i> cells. </h8> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 20:12, 30 October 2017
Results
Rose and Limonene Fragrance
Rose Fragrance
The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10). The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR. The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.
The overlap PCR products were assembled by Gibson Assembly (see protocols) in a pSB1K3 backbone. The assembled rose plasmids were sucessfully transformed into DH5alpha E.coli cells.
HIER TEXT REINKOPIEREN
HIER TEXT REINKOPIEREN
