Difference between revisions of "Team:Stuttgart/Keratinases"
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<p>BCA Assay Results</p> | <p>BCA Assay Results</p> | ||
| − | <br>Protein concentration of supernatant and cell disruption of <i>E.coli</i> carrying different keratinase plasmids (KerP, KerUS and KerA) was measured with BCA Assay method (see chapter methods). | + | <br>Protein concentration of supernatant and cell disruption of <i>E.coli</i> carrying different keratinase plasmids (KerP, KerUS and KerA in different vectors) was measured with BCA Assay method (see chapter methods). |
The picture below shows the BSA standard curve (blank corrected, arithmetic average of triplicates):<br> | The picture below shows the BSA standard curve (blank corrected, arithmetic average of triplicates):<br> | ||
<img src="https://static.igem.org/mediawiki/2017/7/75/Stgt_bcastd.png"/> <br></P> | <img src="https://static.igem.org/mediawiki/2017/7/75/Stgt_bcastd.png"/> <br></P> | ||
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| − | <p> | + | <p>Table 7 and 8 show the results of sample measurements (OD values: blank corrected, arithmetic average of triplicates).<br> The concentrations are calculated with the calibration curve (BSA standard curve).</p> |
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Revision as of 22:07, 30 October 2017
Results
Keratinases
HIER TEXT REINKOPIEREN
TEXT
| sample | OD562 | concentration [µg/mL] |
|---|---|---|
| KerP psD1K3 | 0,514 | 11075,000 |
| KerP psD1C3 | 0,611 | 13491,667 |
| KerUS pet28 | 0,405 | 8358,333 |
| KerUS psD1C3 | 0,285 | 5350,000 |
| KerUS BB | 0,385 | 7841,667 |
| KerA pet28 | 0,482 | 10266,667 |
| KerA psB1C3 | 0,585 | 12858,333 |
| KerA BB | 0,410 | 8475,000 |
| wild type E.coli | 0,615 | 13600,000 |
BCA Assay Results
Protein concentration of supernatant and cell disruption of E.coli carrying different keratinase plasmids (KerP, KerUS and KerA in different vectors) was measured with BCA Assay method (see chapter methods). The picture below shows the BSA standard curve (blank corrected, arithmetic average of triplicates):
| sample | OD562 | concentration [µg/mL] |
|---|---|---|
| KerP psD1K3 | 0,194 | 3066,667 |
| KerP psD1C3 | 0,195 | 3091,667 |
| KerUS pet28 | 0,191 | 2991,667 |
| KerUS psD1C3 | 0,207 | 3391,667 |
| KerUS BB | 0,226 | 3866,667 |
| KerA pet28 | 0,194 | 3083,333 |
| KerA psB1C3 | 0,213 | 3550,000 |
| KerA BB | 0,201 | 3241,667 |
| wild type E.coli | 0,248 | 4416,667 |
Table 7 and 8 show the results of sample measurements (OD values: blank corrected, arithmetic average of triplicates).
The concentrations are calculated with the calibration curve (BSA standard curve).
