Difference between revisions of "Team:BostonU HW/Culturing"

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<title>Cell Lysis</title>
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<title>PCR</title>
  
 
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background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
 
<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
 
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
 
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<img src="https://static.igem.org/mediawiki/2017/3/33/MARS_Cell_Lysis.png" id="TITLE">
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<img src="https://static.igem.org/mediawiki/2017/8/85/MARS_PCR.png" id="TITLE">
 
</div>
 
</div>
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<div class="container">
 
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<div class="col-md-9">
 
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<h1>Summary</h1>
 
<h1>Summary</h1>
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Cell lysis is a commonly used protocol in synthetic biology. It can be performed through a variety of different methods, however we had focused on chemical cellular lysis. Cell Lysis is used to extract and isolate DNA from a specific type of cell. This is an extremely important step in building genetic circuits in order to utilize specific coding regions in a cell's DNA. Chemical cell lysis involves introducing cells to a series of buffers in order to degrade the cell’s outer membrane and collect the DNA that is released.
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PCR, or polymerase chain reaction, is a commonly used protocol in synthetic biology in order to replicate target DNA fragments. Through the use of specific primers, polymerases, and temperature cycling, exponential replicas of the target DNA fragment can be made. These fragments can then be ligated into plasmids and transformed into bacteria.
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This microfluidic chip is designed to perform chemical cell lysis. Suspended cells and an lysing buffer would be mixed inside the cell. This mixture of buffer and cells would be then mixed with a neutralization buffer to prevent DNA degradation. The mixture would then move to the diamond chamber where the DNA binds magnetic particles. Lastly, an elution buffer would be input and to clean and release the DNA from the magnetic particles.
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<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" >
 
<a href="MARStest.zip"download>
 
<a href="MARStest.zip"download>
 
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
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This microfluidic chip is designed to perform PCR. A solution containing the required substrates for PCR is flowed into the chip and through a mixer, on top of chip a heating element will be placed. The valves at both ends of the mixer are closed, isolating the mixture, and then the heating element performs temperature cycling. The liquid is then pushed out into an output receptacle for future use in the lab.
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                                <br><br>
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This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
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<img class="pics" src="https://static.igem.org/mediawiki/2017/b/bb/MARS_Lysis_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/8/87/MARS_PCR_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/e/e3/MARS_PCR_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/08/MARS_Lysis_M.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="tab-pane" id="Mill">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/a/a6/MARS_PCR_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png" alt="Picture">
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
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<img class="pics" src="https://static.igem.org/mediawiki/2017/4/47/MARS_PCR_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center">
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<button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button>
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</div>
 
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<!-- THIS IS FOOTER -->
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<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
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<video style="width:100%; margin-top:20px; padding-top:18px;" controls>
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  <source src="https://static.igem.org/mediawiki/2017/4/47/MARS_LysisVid.mp4" type="video/mp4">
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<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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<h3>CONTACT US</h3>
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<div style="text-align:center;">
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<h1>Testing</h1>
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<a href="mailto:igembuhw@gmail.com">
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<div class="text">
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<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
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</a>
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No biological material was inserted into this chip.
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<a href="https://www.instagram.com/buigemhardware/?hl=en">
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<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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<a href="https://twitter.com/igemhwbu">
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<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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</a>
 
</div>
 
</div>
 
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<div class="col-md-10" style="margin-bottom:30px;">
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<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
 
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Revision as of 00:25, 31 October 2017

BostonU_HW

PCR

Summary

PCR, or polymerase chain reaction, is a commonly used protocol in synthetic biology in order to replicate target DNA fragments. Through the use of specific primers, polymerases, and temperature cycling, exponential replicas of the target DNA fragment can be made. These fragments can then be ligated into plasmids and transformed into bacteria.
This microfluidic chip is designed to perform PCR. A solution containing the required substrates for PCR is flowed into the chip and through a mixer, on top of chip a heating element will be placed. The valves at both ends of the mixer are closed, isolating the mixture, and then the heating element performs temperature cycling. The liquid is then pushed out into an output receptacle for future use in the lab.

This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
Picture
Picture
Picture
Picture