Difference between revisions of "Team:Sydney Australia/Composite Part"

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<h3>★  ALERT! </h3>
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@font-face {
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Composite Parts</h1>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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</p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<br>
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<div class="highlight">
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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    <img src="https://static.igem.org/mediawiki/2017/e/ee/T--Sydney_Australia--CompositePartsBanner.gif" width="70%">
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<div class = "content" id="please" width="80%">
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<center><h1>COMPOSITE PARTS</h1></center>
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<center><div class="box5"></div></center>
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<center><h4>All of our gold, silver and bronze winning parts are composite parts, consisting of a multitude of basic parts<br><br> The complete sequences and characteristics of these basic parts are linked on the Parts Registry pages of our composite parts which are <b>linked in each table.</b></h4></center>
 
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<div class = "row">
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<div class = "content" id="please">
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<center><h1>GOLD</h1></center>
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<center><div class="box1"></div></center>
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<div class="row" style="height:2vw;"></div>
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
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  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
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  solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME /<br> NO.<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>SOURCE<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>KEY FEATURES<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>WHY IS IT GOLD?<o:p></o:p></span></p>
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  </td>
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<tr style="mso-yfti-irow:1;height:78.9pt">
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  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
 +
  </td>
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  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br>Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
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  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
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  (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
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  translocate protein into media<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
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  purification<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
 +
  demonstrated that it works:<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
 +
  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
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  glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
 +
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 +
  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
 +
  ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
 +
  the media in which the cells were growing did. <span class="GramE">Therefore</span>
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  the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
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  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
 +
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
 +
  </td>
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</tr>
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<tr style="mso-yfti-irow:2;height:206.35pt">
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  <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
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 +
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br>E. Coli (<span class="SpellE">Ecotin</span>
 +
  tag)<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
 +
  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
 +
  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
 +
  site<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 +
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 +
  <!--[endif]--><o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
 +
  improved previous parts:<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Completing their sequence<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
 +
  transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
 +
  site<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
 +
  for purification<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
 +
  demonstrated that it works:<o:p></o:p></span></p>
 +
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
 +
  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
 +
  glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
 +
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:3;height:72.85pt">
 +
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br>Human (<span class="SpellE">Human</span>
 +
  proinsulin)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
 +
  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
 +
    <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
        <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
          <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br<br><br>Cytoplasmic-Proinsulin <span style="mso-spacerun:yes">&nbsp;&nbsp;&nbsp;&nbsp;</span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Human (<span class="SpellE">Human</span>
 +
  proinsulin)<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
 +
  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
 +
  site<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
 +
  improved previous parts:<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Completing their
 +
  sequence<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
 +
  ribosomal binding site<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
 +
  N-terminal His tag for purification<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
 +
  font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
 +
  cell lines and saw an increase in glycogen synthesis and glucose oxidation
 +
  above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
 +
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
</div>
 +
</div>
 +
 +
<div class="row" style="height:5vw;"></div>
 +
 +
<div class = "row">
 +
<div class = "content" id="please">
 +
<center><h1>SILVER</h1>
 +
<div class="box2"></div></center>
 +
<div class="row" style="height:2vw;"></div>
 +
<div align="center">
 +
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
 +
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
 +
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
 +
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
 +
  solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:67.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME /<br> NO.</span><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
 +
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
 +
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
 +
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
 +
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
    <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="12%" style="width:12.56%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
 +
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
 +
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="30%" style="width:30.12%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
 +
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
 +
  parts by showing that they work in producing insulin. We performed an ELISA
 +
  assay testing for properly folded insulin, and detected the presence of
 +
  Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
 +
  results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="row" style="height:5vw;"></div>
 +
 +
<div class = "row">
 +
<div class = "content" id="please">
 +
<center><h1>BRONZE</h1>
 +
<div class="box3"></div></center>
 +
<div class="row" style="height:2vw;"></div>
 +
<div align="center">
 +
<div align="center">
 +
 +
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
 +
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
 +
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
 +
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
 +
  solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME /<br> NO.<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
 +
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
 +
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
 +
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:1;height:35.15pt">
 +
  <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
 +
  </td>
 +
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>E. Coli (<span class="SpellE">Ecotin</span>
 +
  Tag)<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
 +
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
 +
  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
 +
  the registry, for other teams to use in the future<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
 +
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
 +
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
 +
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="row" style="height:10vw;"></div>
  
 +
</body>
 
</html>
 
</html>
 +
{{:Team:Sydney_Australia/templates/Footer}}

Revision as of 02:31, 31 October 2017

COMPOSITE PARTS

All of our gold, silver and bronze winning parts are composite parts, consisting of a multitude of basic parts

The complete sequences and characteristics of these basic parts are linked on the Parts Registry pages of our composite parts which are linked in each table.

GOLD

 

PART NAME /
NO.

 


SOURCE


KEY FEATURES



WHY IS IT GOLD?











YncM-Winsulin

BBa_K2417005

 









Bacillus subtilis (YncM secretion tag)

 

 

Novel short chain insulin analogue (Winsulin) with a 12AA linker
(-QRGGGSGGGQRR-)

 

YncM secretion tag to translocate protein into media

 

N-terminal 6x His Tag purification

 

4x GSS flexible linker

 

TEV protease cleavage site

 

Extended ribosome binding site

 

 

 

We demonstrated that it works:

·      We tested it in insulin-sensitive cell lines and saw an increase in glycogen synthesis and glucose oxidation above basal levels, proving its bioactivity

·      We showed via an ELISA assay that whilst the cell lysate did not contain Winsulin, the media in which the cells were growing did. Therefore the YncM secretion tag worked as expected

 

The results for these assays can be found here











Ecotin-Proinsulin

BBa_K2417000

 









E. Coli (Ecotin tag)

 

 

 

Human proinsulin

 

N-terminal Ecotin tag for targeting proteins to the periplasm

 

N-terminal 6x His tag

 

4x GGS flexible linker

 

Trypsin protease cleavage site for His-tag and C-peptide cleavage

 

Extended ribosomal binding site

 



We improved previous parts:

BBa_M39904 and BBa_M1877 by:

 

Completing their sequence

 

Adding a periplasmic transporter tag (for more efficient folding in an oxidative environment)

 

Adding an efficient ribosomal binding site

 

Adding an N-terminal His tag for purification

 

We demonstrated that it works:

·      By testing it insulin-sensitive cell lines and saw an increase in glycogen synthesis and glucose oxidation above basal levels, therefore proving its bioactivity

 

The results for these assays can be found here







Human (Human proinsulin)

 

 

 

 




Cytoplasmic-Proinsulin     BBa_K2417001








Human (Human proinsulin)

Human proinsulin

 

N-terminal 6x His tag

 

4x GGS flexible linker

 

Trypsin protease cleavage site for His-tag and C-peptide cleavage

 

Extended ribosomal binding site

 

We improved previous parts:

BBa_M39904 and BBa_M1877 by:

·      Completing their sequence

·      Adding an efficient ribosomal binding site

·      Adding an N-terminal His tag for purification

 

We demonstrated that it works:

·      By testing it insulin-sensitive cell lines and saw an increase in glycogen synthesis and glucose oxidation above basal levels, therefore proving its bioactivity

 

The results for these assays can be found here

SILVER

 

PART NAME /
NO.

 



SOURCE



KEY FEATURES



WHY IS IT SILVER?

 

 

 

 

 

Cytoplasmic-Winsulin

BBa_K2417003

 



N/A – Winsulin is self designed from literature and mammalian insulins

 

 

Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

 

N-terminal 6x His Tag

 

4x GSS flexible linker

 

TEV protease cleavage site

 

Extended ribosome binding site

 

 

 

·      We validated our parts by showing that they work in producing insulin. We performed an ELISA assay testing for properly folded insulin, and detected the presence of Cytoplasmic Winsulin.

 

 

The results for the assay can be found here

 

 

BRONZE

 

PART NAME /
NO.

 



SOURCE



KEY FEATURES



WHY IS IT BRONZE?

 

 

 

 

 

 

Ecotin-Winsulin

BBa_K2417002

 



E. Coli (Ecotin Tag)

 

 

Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

 

N-terminal Ecotin tag for targeting proteins to the periplasm

 

N-terminal 6x His Tag

 

4x GSS flexible linker

 

TEV protease cleavage site

 

Extended ribosome binding site

 

 

 

 

 

 

 

We submitted this DNA part to the registry, for other teams to use in the future

 

N/A – Winsulin is self designed from literature and mammalian insulins