Difference between revisions of "Team:Stuttgart/Composite Part"
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Many different keratinases produced by different microorganisms (e.g. <i>Bacillus subtilis</i>) have been reported. | Many different keratinases produced by different microorganisms (e.g. <i>Bacillus subtilis</i>) have been reported. | ||
These enzymes vary in their biochemical and biophysical properties e.g. temperature and pH activity range. | These enzymes vary in their biochemical and biophysical properties e.g. temperature and pH activity range. | ||
| + | KerP is a keratunase originating from <i>Pseudomonas aeruginosa</i> It was succesfully transformed and expressed as an extracellular protein in <i>E.coli</i> with a size of 33 kDa and a specific activity of 3,7 kU/mg. | ||
| + | This keratinase belongs to the group of serine proteases with optimal activity at pH 9 and 50°C (Sharma and Gupta 2010). The keratinase was synthesized by IDT (Integrated DNA Technologies, BVBA, Belgium). | ||
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</div> | </div> | ||
Revision as of 13:55, 31 October 2017
Composite Part
KerP from Pseudomonas aeruginosa
Keratinases are proteolytic enzymes that are applied in agro-industrial, pharmaceutical and biomedicals fields. In our project we used keratinases for establishing a microbial tube cleaner. Hair mostly consists of alpha-keratin which is hydrolyzed by keratinases. By using the keratinases we want to avoid chemical compounds that are recently present in tube cleaners. Many different keratinases produced by different microorganisms (e.g. Bacillus subtilis) have been reported. These enzymes vary in their biochemical and biophysical properties e.g. temperature and pH activity range. KerP is a keratunase originating from Pseudomonas aeruginosa It was succesfully transformed and expressed as an extracellular protein in E.coli with a size of 33 kDa and a specific activity of 3,7 kU/mg. This keratinase belongs to the group of serine proteases with optimal activity at pH 9 and 50°C (Sharma and Gupta 2010). The keratinase was synthesized by IDT (Integrated DNA Technologies, BVBA, Belgium).
