Difference between revisions of "Team:NYMU-Taipei/Contribution"
| Line 223: | Line 223: | ||
<div class='right_column'> | <div class='right_column'> | ||
<h2>Constructs & Transformants</h2> | <h2>Constructs & Transformants</h2> | ||
| − | <p>We sent 14 parts to iGEM and successfully transformed pigments into <i>Synechococcus | + | <p>We sent 14 parts to iGEM and successfully transformed pigments into <i>Synechococcus elongatus</i> PCC 7942.</p> |
<div id='s4' class='expandable' style='height:32px;'> | <div id='s4' class='expandable' style='height:32px;'> | ||
| Line 229: | Line 229: | ||
<font class='h7'>Yellow Microalgae with CrtZ </font> | <font class='h7'>Yellow Microalgae with CrtZ </font> | ||
</a> | </a> | ||
| − | <p style='padding-top:0px;'>We have successfully used our microalgae transformation platform to transform CrtZ into <i>Synechococcus | + | <p style='padding-top:0px;'>We have successfully used our microalgae transformation platform to transform CrtZ into <i>Synechococcus elongatus</i> PCC 7942 and the transformant are more yellow than wild type! <a href='https://2017.igem.org/Team:NYMU-Taipei/Pigments'>(see more detail: Pigments - Functional Test)</a> |
</p> | </p> | ||
</div> | </div> | ||
| Line 290: | Line 290: | ||
<font class='h7'>Chung Cheng University – Fusion PCR</font> | <font class='h7'>Chung Cheng University – Fusion PCR</font> | ||
</a> | </a> | ||
| − | <p style='padding-top:0px;'>In 2016, NYMU-Taipei team helped students from National Chung Cheng University to form their iGEM team. | + | <p style='padding-top:0px;'>In 2016, NYMU-Taipei team helped students from National Chung Cheng University to form their iGEM team. Excitedly, they are going to participate iGEM competition this year as team name “CCU_Taiwan”. In 2017, we showed them how the Photo-Bioreactor functions, taught them how to run Fusion PCR through webcam, and informed them some team operations and matters. |
</p> | </p> | ||
</div> | </div> | ||
Revision as of 14:07, 31 October 2017
Contribution
Microalgae Transformation Platform
To transform pigments genes into Synechococcus elongatus PCC 7942, we created a well-designed platform of microalgae transformation.
Constructs & Transformants
We sent 14 parts to iGEM and successfully transformed pigments into Synechococcus elongatus PCC 7942.
Modeling
We established 13 models to check and predict the results of the experiments. These models are extremely important to our project! (see more detail: Modeling)
Unique and Detailed Protocols for the Public
We provided unique and detailed protocols of our experiments to the public. Students, teachers, scientists all over the world and future iGEM teams can repeat our experiments or design their own experiments with these protocols. (see more detail: Notebook - Protocols)
Sharing Genetic Engineering Technology
Assistance to Establish New iGEM Teams
We help students in Chung Hsing University and Taipei Wego Senior High School to establish their own iGEM teams. We are looking forward to seeing them at Boston in 2018!
Experimental Technology
In addition to our comprehensive microalgae transformation platform, to achieve our goals, we also applied lots of experimental technology. Although most of our team members are freshman in college, we tried hard to learn relatively complicated experimental methods. Moreover, we realized that, with more experimental technology and knowledge, we can be closer to our goals and do more things for the world. Therefore, we strongly encourage future iGEM team to explore new technology. Deeper thinking and thrive on challenge that come along with the journey of learning are as important as the technology itself.
Fusion PCR
We used fusion PCR in NrtA construct, pPIGBACK construct and fusion of pigments and the promoter prbcL.
Sticky End PCR
We used sticky end PCR in holin, endolysin, NrtA and CrtZ construct.
3 Piece Overlap PCR
We used 3 piece overlap PCR in lycopene and pPIGBACK construct.
Site Directed Mutagenesis
We used site directed mutagenesis in pPIGBACK and PrbcL construct.
Electroporation
We used electroporation in IndC construct.
Making Competent Cell
We made competent cell in electroporation experiments.
French Pressure Cell Press NrtA
We use French pressure cell press in NrtA functional test.
