Difference between revisions of "Team:REC-CHENNAI/insilico work"

Latest revision as of 14:49, 31 October 2017

INSILICO WORK

INTRODUCTION

We carried out insilico studies for both our peptides using ExPASy ProtParam tool and the results are as listed below.

NATIVE LATARCIN

Peptide Sequence: GLFGKLIKKFGRKAISYAVKKARGKH-OH

Number of amino acids: 26
Molecular weight: 2902.57
Theoretical pI: 11.33

Atomic composition

Carbon	C	136
Hydrogen	H	229
Nitrogen	N	41
Oxygen	O	29
Sulfur	S	0

Formula: C₁₃₆H₂₂₉N₄₁O₂₉

Total number of atoms: 435

Estimated half-life

The N-terminal of the sequence considered is G (Gly).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index

The instability index (II) is computed to be 1.29
This classifies the protein as stable.
Aliphatic index: 82.69
Grand average of hydropathicity (GRAVY): -0.438

MUTANT LATARCIN

Peptide sequence: GLFGKLIKKKGRKAISYAVKKARGKH-OH

Number of amino acids: 26
Molecular weight: 2883.57
Theoretical pI: 11.36

Atomic composition

Carbon	C	133
Hydrogen	H	232
Nitrogen	N	42
Oxygen	O	29
Sulfur	S	0

Formula: C₁₃₃H₂₃₂N₄₂O₂₉

Total number of atoms: 436

Estimated half-life

The N-terminal of the sequence considered is G (Gly).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index

The instability index (II) is computed to be -1.98
This classifies the protein as stable.
Aliphatic index: 82.69
Grand average of hydropathicity (GRAVY): -0.696