Difference between revisions of "Team:REC-CHENNAI/Description"
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<h1>QUORUM SENSING</h1> | <h1>QUORUM SENSING</h1> | ||
| − | <p>Our project involves genetically engineering <i>E.coli DH5a </i> cells to produce Latarcin and the mutant. We chose <i> E.coli </i> to be our chassis due to its higher transformation efficiency. However, | + | <p>Our project involves genetically engineering <i>E.coli DH5a </i> cells to produce Latarcin and the mutant. We chose <i> E.coli </i> to be our chassis due to its higher transformation efficiency. However, the problem with making <i>E.coli</i> produce this peptide is the toxicity of this peptide to the chassis itself. In order to overcome this, a specially designed quorum sensing mechanism is employed. </p> |
<p> Bacterial quorum sensing is defined as a cell-to-cell communication mechanism wherein gene expression is regulated by fluctuations in cell concentration. Bacteria that use quorum sensing mechanisms release certain signalling molecules known as autoinducers into their surroundings. Beyond a threshold limit, these autoinducers alter gene expression by binding to specific promoters within the genome. </p> | <p> Bacterial quorum sensing is defined as a cell-to-cell communication mechanism wherein gene expression is regulated by fluctuations in cell concentration. Bacteria that use quorum sensing mechanisms release certain signalling molecules known as autoinducers into their surroundings. Beyond a threshold limit, these autoinducers alter gene expression by binding to specific promoters within the genome. </p> | ||
<p> Quorum sensing is an essential element in our project because the Latarcin that is produced by the E. coli cells is deadly to the bacteria itself. This will not allow for large scale production of Latarcin. When the quorum sensing mechanism is incorporated, Latarcin production will begin only after the cells reach a high concentration. Thus, large quantities of Latarcin can be synthesized. </p> | <p> Quorum sensing is an essential element in our project because the Latarcin that is produced by the E. coli cells is deadly to the bacteria itself. This will not allow for large scale production of Latarcin. When the quorum sensing mechanism is incorporated, Latarcin production will begin only after the cells reach a high concentration. Thus, large quantities of Latarcin can be synthesized. </p> | ||
Revision as of 16:24, 1 November 2017
