Difference between revisions of "Team:Georgia State/HP/Silver"

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<div class="media-body"><img  src="https://static.igem.org/mediawiki/2017/5/51/T--Georgia_State--LALFACILITY.jpg" height="auto" width="auto" alt="">
 
<div class="media-body"><img  src="https://static.igem.org/mediawiki/2017/5/51/T--Georgia_State--LALFACILITY.jpg" height="auto" width="auto" alt="">
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<br><h1 class="media-heading">Sources</h1>
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<p class="last"> Botton ML Shuster CN Jr Keinath J . 2003. Horseshoe crabs in a food web: Who eats whom. Pages. 33-153. in Shuster CN Jr, Barlow RB, Brockmann HJ, eds. The American Horseshoe Crab . Cambridge (MA): Harvard University Press.</p>
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<p class="last"> Carmichael RH Rutecki D Annett B Gaines E Valiela I . 2004. Position of horseshoe crabs in estuarine food webs: N and C stable isotopic study of foraging ranges and diet composition. Journal of Experimental Marine Biology and Ecology . 299: 231-253.</p>
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<p class="last"> Castro G Myers JP . 1993. Shorebird predation on eggs of horseshoe crabs during spring stopover on Delaware Bay. The Auk . 110: 927-930.</p>
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<p class="last"> Castro G Myers JP . 1993. Shorebird predation on eggs of horseshoe crabs during spring stopover on Delaware Bay. The Auk . 110: 927-930.</p>
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<p class="last"> Jay Odell, Martha E. Mather, Robert M. Muth; A Biosocial Approach for Analyzing Environmental Conflicts: A Case Study of Horseshoe Crab Allocation, BioScience, Volume 55, Issue 9, 1 September 2005, Pages 735–748, https://doi.org/10.1641/0006-3568(2005)055[0735:ABAFAE]2.0.CO;2</p>
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<p class="last"> Office, U. F. (2006). The Horseshoe Crab Limulus polyphemus A Living Fossil. </p>
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<h1 class="media-heading">Picture Sources</h1>
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<p class="last"> Source for LAL processing facility: G. Riekerk, SCDNR Marine Resources Research Institute</p>
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<p class="last"> Source for eel from free media commons: https://commons.wikimedia.org/wiki/File:Knobbed_whelk_shells.jpg</p>
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<p class="last"> Source for eel from free media commons: https://commons.wikimedia.org/wiki/File:California_Moray_Eel,_San_Clemente_Island,_Channel_Islands,_California.jpg</p>
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<p class="last"> The picture for the bleeding horse shoe crabs comes from an Atlantic article, the picture is a a still from the PBS Nature documentary Crash PBS. The image and the article can be located at this source: https://www.theatlantic.com/technology/archive/2014/02/the-blood-harvest/284078/</p>
 
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<h3> Biomedical Threats</h3>
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h1 class="media-heading">Biomedical Threats</h1>
    <p class="last">Horseshoe crabs do not only face the threat of being used for bait but instead, have a more financially driven predator in the form of the biomedical industry.  If you have ever had a flu shot, known someone with a pacemaker or joint replacement, or given your pet a rabies vaccination, you owe a debt of gratitude to the horseshoe crab. All of these devices are quality checked for safety using a test that comes from the blood of the horseshoe crab. The blood of the crab is very sensitive to endotoxins, the blood from the crab is used commercially to develop the LAL and TAL test. The Limulus amebocyte lysate (LAL)test and was commercialized in the United States in the 1970s and Asia, there is a similar test called TAL which takes its name from an Asian species of crab, Tachypleus tridentatus (Jay Odell, 2005). The market for LAL is approximately $50 million (Focus, 2008). Each year,  half a million living horse crabs are harvested to develop these endotoxin tests- for these kits, companies take 30 percent or more of the horseshoe crabs blood. Experts estimate that 10-15% of 500,000 crabs harvested on the Atlantic coast do not survive the bleeding process (Focus, 2008). Currently, no synthetic substitute has the same accuracy as the LAL test, so crab blood must be used, and thus the threat persists (Focus, 2008).
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        <p class="last">Horseshoe crabs do not only face the threat of being used for bait but instead, have a more financially driven predator in the form of the biomedical industry.  If you have ever had a flu shot, known someone with a pacemaker or joint replacement, or given your pet a rabies vaccination, you owe a debt of gratitude to the horseshoe crab. All of these devices are quality checked for safety using a test that comes from the blood of the horseshoe crab. The blood of the crab is very sensitive to endotoxins, the blood from the crab is used commercially to develop the LAL and TAL test. The Limulus amebocyte lysate (LAL)test and was commercialized in the United States in the 1970s and Asia, there is a similar test called TAL which takes its name from an Asian species of crab, Tachypleus tridentatus (Jay Odell, 2005). The market for LAL is approximately $50 million (Focus, 2008). Each year,  half a million living horse crabs are harvested to develop these endotoxin tests- for these kits, companies take 30 percent or more of the horseshoe crabs blood. Experts estimate that 10-15% of 500,000 crabs harvested on the Atlantic coast do not survive the bleeding process (Focus, 2008). Currently, no synthetic substitute has the same accuracy as the LAL test, so crab blood must be used, and thus the threat persists (Focus, 2008).
 
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<img src="https://static.igem.org/mediawiki/2017/5/52/T--Georgia_State--horseshoecrabbleeding.jpg" alt="">   
 
<img src="https://static.igem.org/mediawiki/2017/5/52/T--Georgia_State--horseshoecrabbleeding.jpg" alt="">   

Revision as of 16:43, 1 November 2017