Difference between revisions of "Team:REC-CHENNAI/Results"

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<h1>PROBLEM STATEMENT</h1>
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<p> We carried out transformation using <i>E.coli Dh5a </i> cells. Three different test concentration of the plasmid was used- 100 pg/µL, 50 pg/µL, 10 pg/µL to estimate the transformation efficiencies. The plasmid contained RFP in a psb1c3 plasmid backbone. The transformation efficiency for each concentration is listed below. </p>
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<p>The emergence of multi-drug resistance in food-borne bacteria is an increasing problem globally. Described by WHO, CDC and other International agencies as one of the world’s most pressing public health threats, antibiotic resistant infections from food-borne bacteria cause an estimated 430,000 illnesses each year in the USA alone. This issue thus has attracted significant attention towards Antimicrobial peptides (AMPs).</p>
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<p> We have also plotted  a standard curve for the growth of <i>E.coli Dh5a </i> cells. When the cells were transformed with a plasmid containing latarcin, we were unable to obtain mature cells due to the toxicity of the peptide to the host. The growth curve for cells containing latarcin is also shown below. </p> </div>
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<p>The emergence of multi-drug resistance in food-borne bacteria is an increasing problem globally. Described by WHO, CDC and other International agencies as one of the world’s most pressing public health threats, antibiotic resistant infections from food-borne bacteria cause an estimated 430,000 illnesses each year in the USA alone. This issue thus has attracted significant attention towards Antimicrobial peptides (AMPs).</p>
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<h1>ABOUT OUR PEPTIDE</h1>
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<p>To overcome this problem, we designed the quorum sensing mechanism. However, due the time constraint, we were unable to successfully express the protein and test its functionality. </p> </div>
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<h1>PROBLEM STATEMENT</h1>
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<p>The emergence of multi-drug resistance in food-borne bacteria is an increasing problem globally. Described by WHO, CDC and other International agencies as one of the world’s most pressing public health threats, antibiotic resistant infections from food-borne bacteria cause an estimated 430,000 illnesses each year in the USA alone. This issue thus has attracted significant attention towards Antimicrobial peptides (AMPs).</p>
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Revision as of 19:33, 1 November 2017