Difference between revisions of "Team:Bielefeld-CeBiTec/Demonstrate"
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Revision as of 22:32, 1 November 2017
Demonstrate Your Work
To use these new codons, we developed a library of over eight-thousand synthetase sequences and a positive/negative selection system to obtain new aminoacyl-tRNA-synthetases. These can be applied to charge non-canonical amino acids to the tRNA against the novel codon and to turn semi-synthetic codons functional.
To demonstrate the benefits of non-canonical amino acids to the synthetic biology community, we worked on five applications utilizing non-canonical amino acids. Furthermore, we designed and synthetized our own fully synthetic non-canonical amino acid and modeled possible synthetase-sequences for its incorporation. We also improved a test system and defined a ranking system for aminoacyl-tRNA synthetases
We are convinced that we have laid the foundations for a whole new field of synthetic biology for the iGEM community. We would be very honored if future teams would build on our project to further develop this approach and to develop new and exciting applications!
Achievements
Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via Oxford Nanopore sequencing and an enzyme based detection method
Development of a software suite for these orthogonal methods
Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates
Proof that certain Taq-polymerases can efficiently incorporate unnatural nucleotides
Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids
Colocalization of the RuBisCo using a fluorescent amino acid
Development of a photoswitchable lycopene pathway
Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine
Modeling more than ten new aaRS sequences
Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases
Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids
Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system
Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and much more
Development of an Android App to control the LED panel with your smartphone via Bluetooth
Writing a biosafety report titled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”
Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”
