Difference between revisions of "Team:Kent/Contribution"

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<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb1">Production of Lysogeny broth (LB)</label>
+
<label class="hull-title" for="cb1">Tarek Asfour</label>
 
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<div class="hull-content">For 1 litre of LB a mixture of 10g of sodium chloride, 10g peptone, 5g of yeast extract as well as 1
+
<div class="hull-content">Tarek contributed strongly in the development of our brand designs. He coded the website, designed and perfected the poster and has developed and was responsible for the Team Kent branded clothes. He also did about all the visual artifacts that can be found on our website and poster and the boing! fair. Tarek was crucial to the development of the wiki and designing all the graphics by hand that were used in public events, the wiki and the poster.</div>
litre of distilled water in a glass bottle. We then used a magnetic spinner to help mix the powders
+
with the water, we avoided shaking the glass bottle as it would cause froth and waste some of the
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LB.
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<br>
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When making the LB we also made another litre batch and added 15g of agar extract to be able to
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grow bacteria on plates.</div>
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</section>
 
</section>
 
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<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb2">Production of SOB medium and magnesium stock</label>
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<label class="hull-title" for="cb2">Abdul Chowdhury</label>
 
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<div class="hull-content">Bringing together 20g of tryptone, 5g of yeast extract, 0.584g of NaCl, 0.186g of KCl and mixing it
+
<div class="hull-content">Abdul developed and worked out all the mathematical modelling of our project. He worked out the equations and created 3D pymol images of our system, and also helped out with the dry lab side of the project by helping at the bO!ng International Family Festival at the Gulbenkian, University of Kent. </div>
with 990ml of millipure water (using the magnetic mixer again) which was then put in to autoclave
+
to sterilise it, after it was taken out and let for it to cool down to below 60 o C.
+
<br>
+
10ml of 2M Mg 2+ stock was then added and then brought to 100ml with millipure water, 0.2mm
+
filter sterilize was then used</div>
+
 
</section>
 
</section>
 
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<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb3">Production of SOC medium and glucose stock</label>
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<label class="hull-title" for="cb3">Laulwa Al Salloum</label>
 
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<label class="hull-close" for="acc-close"></label>
<div class="hull-content">Once again bring 20g of tryptone, 5g of yeast of extract, 0.584g of NaCl, 0.186g of KCL, and then
+
<div class="hull-content">Laulwa, as part of the wet lab team, performed most experiments from transformations, competent cell production, microscope imagery, PCR and transfections. She did research for the project design and was one of the team members focussed on the interlab study. Lulu was also responsible for the Facebook page and kept an eye on the expenditures. She also helped at the bO!ng international family festival and was a key asset in regards to our collaboration, helping the Judd High School team complete their interlab study. She worked as a student ambassador during the two open days, in which she helped explain our project to potential university students.
bring 970 ml with millipure water and use the magnetic mixer once again, this was also then put in
+
</div>
to autoclave.
+
<br>
+
10ml of 2M Mg 2+ stock and then bring it to 100ml with milllipure water, filter sterilize it with 0.2m
+
and then final add 20ml of 1M glucose stock.</div>
+
 
</section>
 
</section>
 
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<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb4">Production of Glycerol stock</label>
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<label class="hull-title" for="cb4">Nina Grexova</label>
 
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<label class="hull-close" for="acc-close"></label>
<div class="hull-content">If you wish to store bacteria long term, you will need to create a Glycerol Stock after
+
<div class="hull-content">Nina was part of the wet lab and research team and took part in most labwork. Her main contribution lies in PCR and ligation of gBLOCKS, cloning and the transgene design as well as the research when the project idea was developed. Apart from that Nina was responsible for the Instagram channel and worked out ideas for our presence at the bO!ng International Family Festival at the Gulbenkian, University of Kent. She worked as a student ambassador during an open day at the university, helping explain iGEM and our project to potential university students.</div>
inoculating an overnight liquid culture
+
<br>
+
<ul><li>Once bacterial growth has been achieved, 500μL of the overnight liquid
+
culture needs to be added to 500μL of 50% glycerol in a 2mL tube where it
+
should be gently mixed</li>
+
<li>The glycerol stock should then be frozen at -80 o C<ul>
+
<li> Successive freeze and thaw cycles will reduce the stocks shelf life</li></ul>
+
</li></ul></div>
+
 
</section>
 
</section>
 
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<input type="radio" name="droptext" id="cb5" />
 
<input type="radio" name="droptext" id="cb5" />
 
<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb5">Running Agarose Gel</label>
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<label class="hull-title" for="cb5">Ivy Cheung</label>
 
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<label class="hull-close" for="acc-close"></label>
<div class="hull-content">After the cells have been miniprepped and the plasmid put through a restriction digest, the agarose gel can be run.
+
<div class="hull-content">Ivy was part of the dry lab team and took part in organisation of key public engagement events, such as the bO!ng International Family Festival at the Gulbenkian in the University of Kent. She also contributed to the poster design for the UK team meetup in August, at the University of Westminster.</div>
<br>
+
<ul><li>Make up some agarose. This is done by taking 0.5g of agarose powder and putting it in a
+
250ml sterile conical flask, with 50ml of TAE buffer, then microwaving it in small pulses (20
+
seconds then swirling it around) until it is dissolved. Don’t overheat it or it will evaporate too
+
much. Make up the evaporated volume to 50ml with distilled water.</li>
+
<li>Add 1 vial of cybersafe (ask technical services for a tube of it and add all of it)</li>
+
<li>Line the white sides of the tank with the agarose solution, to seal it and prevent leakage. Use
+
a p1000 pipette set to 1ml. Let it dry (about 5 mins max)</li>
+
<li>Then pour all the agarose/sybrsafe solution into the tank and put in the comb. Let it set and
+
solidify (maximum 30 mins)</li>
+
<li>When the gel has set, remove the comb from the tank (gently!) and then cover the whole
+
tank with TAE buffer, so there’s at least half a centimetre of TAE covering the gel.</li>
+
<li>Now, the samples need to be loaded. Load some DNA markers (ask technical services for a
+
tube of this and load the whole tube) into well 1( left hand side) and then choose what you
+
load into wells 2, 3, and 4 etc. (make sure you note what’s in each lane!)</li>
+
<li>Load all of your digests into the wells 2,3, and 4.</li>
+
<li>Plug into a power supply and put the cover on. Run for 40 mins to an hour at 80v. The amps
+
don’t matter.</li>
+
<li>Once the visible markers have reached the half way point of the tank, turn off the power
+
supply and drain the TAE buffer form the tank. Remove the gel with a spatula and place in a
+
UV imaging box. Take an image of the gel under UV light, save it onto a USB stick.</li></ul></div>
+
 
</section>
 
</section>
 
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<input type="radio" name="droptext" id="cb2" />
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<input type="radio" name="droptext" id="cb6" />
 
<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb2">Production of SOB medium and magnesium stock</label>
+
<label class="hull-title" for="cb6">Harman Sanghera</label>
 
<label class="hull-close" for="acc-close"></label>
 
<label class="hull-close" for="acc-close"></label>
<div class="hull-content">Bringing together 20g of tryptone, 5g of yeast extract, 0.584g of NaCl, 0.186g of KCl and mixing it
+
<div class="hull-content">Harman was part of the dry lab team and was solely responsible for development of several flash game that will improve the function of our project to children and young adults. He also took part in several human practices events such as questionnaires at the fresher’s fayre, and helped design the team shirts. He also lended a hand in the wet lab when one or more team members were not present.</div>
with 990ml of millipure water (using the magnetic mixer again) which was then put in to autoclave
+
to sterilise it, after it was taken out and let for it to cool down to below 60 o C.
+
<br>
+
10ml of 2M Mg 2+ stock was then added and then brought to 100ml with millipure water, 0.2mm
+
filter sterilize was then used</div>
+
 
</section>
 
</section>
<input type="radio" name="droptext" id="cb6" />
+
<input type="radio" name="droptext" id="cb7" />
 
<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb3">Production of SOC medium and glucose stock</label>
+
<label class="hull-title" for="cb7">Dan Brunkow</label>
 
<label class="hull-close" for="acc-close"></label>
 
<label class="hull-close" for="acc-close"></label>
<div class="hull-content">Once again bring 20g of tryptone, 5g of yeast of extract, 0.584g of NaCl, 0.186g of KCL, and then
+
<div class="hull-content">Dan was part of the wet lab team, and was involved in most lab work. He contributed to most experiments such as transformations of cells, creation of competent cells, mammalian cell transfection, microscope imagery, and assisted with PCR reactions. He also aided in the interlab study and was also involved in the bO!ng Festival for the human practices side of the project, and ran the Twitter account. Dan was also responsible for contacting some sponsors, along with presenting at the summer student symposium at the University of Kent, explaining our project to 2nd year students at the university and working at the open days, talking to future university students about iGem and our project. He is one of the presenters in Boston.  
bring 970 ml with millipure water and use the magnetic mixer once again, this was also then put in
+
</div>
to autoclave.
+
<br>
+
10ml of 2M Mg 2+ stock and then bring it to 100ml with milllipure water, filter sterilize it with 0.2m
+
and then final add 20ml of 1M glucose stock.</div>
+
 
</section>
 
</section>
 
<input type="radio" name="droptext" id="acc-close" />
 
<input type="radio" name="droptext" id="acc-close" />
<input type="radio" name="droptext" id="cb4" />
+
<input type="radio" name="droptext" id="cb8" />
 
<section class="hull">
 
<section class="hull">
<label class="hull-title" for="cb7">Production of Glycerol stock</label>
+
<label class="hull-title" for="cb8">Laurens Heling</label>
 
<label class="hull-close" for="acc-close"></label>
 
<label class="hull-close" for="acc-close"></label>
<div class="hull-content">If you wish to store bacteria long term, you will need to create a Glycerol Stock after
+
<div class="hull-content">Laurens was part of both the wet lab and the dry lab team, but had roles extending throughout the project. He took part in most experiments, from molecular biology techniques such as minipreps, transformations and digests to mammalian cell culture and microscopy and was also heavily involved in the interlab study. He was also a key asset to the team in terms of research for the project idea, primer and g-block design and PCR reactions. Laurens also aided in the dry lab side of the project through creation of a series of Vlogs that were uploaded onto the team’s youtube channel, as well as creation of questionnaires for the public engagement area. He also presented at the summer student symposium at the University of Kent, and worked at the open days, explaining to potential students about our project and iGem as a whole. He is one of the presenters in Boston.
inoculating an overnight liquid culture
+
</div>
<br>
+
<ul><li>Once bacterial growth has been achieved, 500μL of the overnight liquid
+
culture needs to be added to 500μL of 50% glycerol in a 2mL tube where it
+
should be gently mixed</li>
+
<li>The glycerol stock should then be frozen at -80 o C<ul>
+
<li> Successive freeze and thaw cycles will reduce the stocks shelf life</li></ul>
+
</li></ul></div>
+
 
</section>
 
</section>
<input type="radio" name="droptext" id="acc-close" />
+
 
<input type="radio" name="droptext" id="cb5" />
+
<section class="hull">
+
<label class="hull-title" for="cb8">Running Agarose Gel</label>
+
<label class="hull-close" for="acc-close"></label>
+
<div class="hull-content">After the cells have been miniprepped and the plasmid put through a restriction digest, the agarose gel can be run.
+
<br>
+
<ul><li>Make up some agarose. This is done by taking 0.5g of agarose powder and putting it in a
+
250ml sterile conical flask, with 50ml of TAE buffer, then microwaving it in small pulses (20
+
seconds then swirling it around) until it is dissolved. Don’t overheat it or it will evaporate too
+
much. Make up the evaporated volume to 50ml with distilled water.</li>
+
<li>Add 1 vial of cybersafe (ask technical services for a tube of it and add all of it)</li>
+
<li>Line the white sides of the tank with the agarose solution, to seal it and prevent leakage. Use
+
a p1000 pipette set to 1ml. Let it dry (about 5 mins max)</li>
+
<li>Then pour all the agarose/sybrsafe solution into the tank and put in the comb. Let it set and
+
solidify (maximum 30 mins)</li>
+
<li>When the gel has set, remove the comb from the tank (gently!) and then cover the whole
+
tank with TAE buffer, so there’s at least half a centimetre of TAE covering the gel.</li>
+
<li>Now, the samples need to be loaded. Load some DNA markers (ask technical services for a
+
tube of this and load the whole tube) into well 1( left hand side) and then choose what you
+
load into wells 2, 3, and 4 etc. (make sure you note what’s in each lane!)</li>
+
<li>Load all of your digests into the wells 2,3, and 4.</li>
+
<li>Plug into a power supply and put the cover on. Run for 40 mins to an hour at 80v. The amps
+
don’t matter.</li>
+
<li>Once the visible markers have reached the half way point of the tank, turn off the power
+
supply and drain the TAE buffer form the tank. Remove the gel with a spatula and place in a
+
UV imaging box. Take an image of the gel under UV light, save it onto a USB stick.</li></ul></div>
+
</section>
+
<input type="radio" name="droptext" id="acc-close" />
+
 
</nav>
 
</nav>
  

Revision as of 03:46, 2 November 2017


Contribution
London Meetup