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− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <article><br> | |
− | + | <b>We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Oxford Nanopore sequencing</a> application and an enzyme based detection method</b> | |
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− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <article><br><br> | |
− | + | <b>Development of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">software</a> suite for these orthogonal methods </b> | |
− | + | </article> | |
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− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="third double"> | |
− | + | <article><br> | |
− | + | <b>Integration and characterization of the <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/uptake_and_biosynthesis">nucleotide transporter PtNTT2</a> from <i>P.tricornutum</i> in <i>E.coli</i> for the uptake of unnatural nucleoside triphosphates</b> | |
− | + | </article> | |
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− | + | <div class="contentline"> | |
− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="third double"> | |
− | + | <article><br><br> | |
− | + | <b>Confirmation that certain Taq DNA polymerases can efficiently <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/preservation_system">incorporate unnatural nucleotides</a> </b> | |
− | + | </article> | |
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− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <div class="third double"> | |
− | + | <article><br><br> | |
− | + | <b>Construction of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox">toolbox</a> consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids</b> | |
− | + | </article> | |
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− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="third double"> | |
− | + | <article><br><br> | |
− | + | <b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/labeling">Colocalization</a> of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid</b> | |
− | + | </article> | |
− | + | </div> | |
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− | + | <div class="contentline"> | |
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− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <article><br><br> | |
− | + | <b>Development of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/photoswitching">photoswitchable lycopene pathway</a></b> | |
− | + | </article> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="contentline"> | |
− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="third double"> | |
− | + | <article><br> | |
− | + | <b>Design, <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/fusing">chemical synthesis</a> and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine</b> | |
− | + | </article> | |
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− | + | </div> | |
− | + | <div class="contentline"> | |
− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <div class="third double"> | |
− | + | <article><br><br> | |
− | + | <b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Model">Modeling</a> more than ten new aaRS sequences</b> | |
− | + | </article> | |
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− | + | <div class="contentline"> | |
− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="third double"> | |
− | + | <article><br><br> | |
− | + | <b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/translational_system/library_and_selection">Library development</a> with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases</b> | |
− | + | </article> | |
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− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="third double"> | |
− | + | <article><br> | |
− | + | <b>Construction of positive and negative <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/translational_system/library_and_selection">selection plasmids</a> for the evolution of new synthetases for non-canonical amino acids</b> | |
− | + | </article> | |
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− | + | <div class="contentline"> | |
− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <div class="third double"> | |
− | + | <article><br><br> | |
− | + | <b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Improve">Improvement</a> of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system</b> | |
− | + | </article> | |
− | + | </div> | |
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− | + | <div class="third"> | |
− | + | <div class="figure small"> | |
− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
− | + | <p class="figure subtitle"></p> | |
− | + | </div> | |
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− | + | <article><br><br> | |
− | + | <b>Construction of an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Hardware">LED panel</a> for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications </b> | |
− | + | </article> | |
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− | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png"> | |
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− | + | </div> | |
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− | + | <article><br><br> | |
− | + | <b>Development of an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Android</a> App to control the LED panel with your smartphone via Bluetooth</b> | |
− | + | </article> | |
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− | <b>Writing a <a target="_blank" href="https://static.igem.org/mediawiki/2017/1/1e/T--Bielefeld-CeBiTec--DKE_Biosafety_Report.pdf">biosafety report</a> | + | <b>Writing a <a target="_blank" href="https://static.igem.org/mediawiki/2017/1/1e/T--Bielefeld-CeBiTec--DKE_Biosafety_Report.pdf">biosafety report</a> entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System” </b> |
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Revision as of 03:47, 2 November 2017
![](https://static.igem.org/mediawiki/2017/7/73/T--Bielefeld-CeBiTec--start-page-screen1.png)
![](https://static.igem.org/mediawiki/2017/c/c4/T--Bielefeld-CeBiTec--start-page-screen2.png)
![](https://static.igem.org/mediawiki/2017/a/ad/T--Bielefeld-CeBiTec--start-page-screen3.png)
![](https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--start-page-screen4.png)
![](https://static.igem.org/mediawiki/2017/0/01/T--Bielefeld-CeBiTec--start-page-screen5.png)
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![](https://static.igem.org/mediawiki/2017/b/b4/T--Bielefeld-CeBiTec--start-page-screen8.png)
Achievements
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an Oxford Nanopore sequencing application and an enzyme based detection method
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a software suite for these orthogonal methods
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Confirmation that certain Taq DNA polymerases can efficiently incorporate unnatural nucleotides
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Colocalization of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a photoswitchable lycopene pathway
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Modeling more than ten new aaRS sequences
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of an Android App to control the LED panel with your smartphone via Bluetooth
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing a biosafety report entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”