Difference between revisions of "Team:UNOTT/Model"
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<h3> Single gene expressing protein and protein undergoing degradation </h3> | <h3> Single gene expressing protein and protein undergoing degradation </h3> | ||
| − | < | + | <h5> Using the Law of Mass Action and assuming there is no radioactive decay </h5> |
<img src="https://static.igem.org/mediawiki/2017/4/48/T--UNOTT--Formula2.png" style= position: fixed; align=center;> | <img src="https://static.igem.org/mediawiki/2017/4/48/T--UNOTT--Formula2.png" style= position: fixed; align=center;> | ||
<img src="https://static.igem.org/mediawiki/2017/6/61/T--UNOTT--Formula3.png" style= position: fixed; align=center;> | <img src="https://static.igem.org/mediawiki/2017/6/61/T--UNOTT--Formula3.png" style= position: fixed; align=center;> | ||
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| − | < | + | <h3> Signal Dependent Expression </h3> |
| − | < | + | <h5> Where S is Signal Dependent and Ko is a low basal expression </h5> |
<img src="https://static.igem.org/mediawiki/2017/0/05/T--UNOTT--Formula4.png" style= position: fixed; align=center;> | <img src="https://static.igem.org/mediawiki/2017/0/05/T--UNOTT--Formula4.png" style= position: fixed; align=center;> | ||
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Revision as of 13:53, 25 July 2017
space space space space space space space
Modelling
When developing Key.Coli, we found it was important to mathematically model possible situations so we could investigate the effects of different situations we might encounter as well as representing every single chemical reaction which could expect.
This information was used by the wet lab to assist them by informing them in what to expect. This was done through the use of Python and Tellurium to create easy to read graphs.
The models weren't perfect as first: refinement from lab results helped to optimize and correct the models
Single gene expressing protein and protein undergoing degradation
Using the Law of Mass Action and assuming there is no radioactive decay
Signal Dependent Expression
Where S is Signal Dependent and Ko is a low basal expression
Relationship between Fluorescent Intensity and GFP Intensity
CHANGE IT TRANSFORMED INSTEAD OF INFECTED. Reference:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430915/
